##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547863_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1687604
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.393184657064097	31.0	31.0	33.0	30.0	34.0
2	31.795075147961253	31.0	31.0	34.0	30.0	34.0
3	31.85978997442528	31.0	31.0	34.0	30.0	34.0
4	35.640041739649824	37.0	35.0	37.0	33.0	37.0
5	35.50844155382424	37.0	35.0	37.0	33.0	37.0
6	35.56074470077103	37.0	35.0	37.0	33.0	37.0
7	35.94113962754295	37.0	35.0	37.0	35.0	37.0
8	35.95147262035407	37.0	35.0	37.0	35.0	37.0
9	37.67899459825883	39.0	37.0	39.0	35.0	39.0
10	37.211943086174244	39.0	37.0	39.0	33.0	39.0
11	36.89514542511158	39.0	37.0	39.0	33.0	39.0
12	36.143216062536	38.0	35.0	39.0	32.0	39.0
13	35.863460266745044	38.0	35.0	39.0	31.0	39.0
14	36.84851837279362	39.0	35.0	41.0	31.0	41.0
15	37.09569128776656	39.0	35.0	41.0	32.0	41.0
16	37.23318740652428	39.0	35.0	41.0	32.0	41.0
17	37.17524134808877	39.0	35.0	41.0	32.0	41.0
18	37.080265868059094	38.0	35.0	41.0	32.0	41.0
19	37.01530157548809	38.0	35.0	41.0	32.0	41.0
20	36.870769445912664	38.0	35.0	41.0	32.0	41.0
21	36.754709635672825	38.0	35.0	41.0	32.0	41.0
22	36.62832453585083	38.0	35.0	41.0	31.0	41.0
23	36.56333713359295	38.0	35.0	40.0	31.0	41.0
24	36.57865648576325	38.0	35.0	40.0	31.0	41.0
25	36.530623890438754	38.0	35.0	40.0	31.0	41.0
26	36.39346849142334	38.0	35.0	40.0	31.0	41.0
27	36.29692866335942	38.0	35.0	40.0	31.0	41.0
28	36.2339203983873	38.0	35.0	40.0	31.0	41.0
29	36.26698976774172	38.0	35.0	40.0	31.0	41.0
30	36.202342492670084	38.0	35.0	40.0	31.0	41.0
31	36.05362928743947	38.0	35.0	40.0	30.0	41.0
32	35.89189169971154	38.0	35.0	40.0	30.0	41.0
33	35.76999224936656	38.0	35.0	40.0	30.0	41.0
34	35.658627260897696	37.0	35.0	40.0	30.0	41.0
35	35.616001147188555	37.0	35.0	40.0	29.0	41.0
36	35.467089435673294	37.0	35.0	40.0	29.0	41.0
37	35.338479287795	37.0	34.0	40.0	28.0	41.0
38	35.27304272803335	37.0	34.0	40.0	28.0	41.0
39	35.21346002972261	37.0	34.0	40.0	27.0	41.0
40	35.040862666834165	37.0	34.0	40.0	26.0	41.0
41	34.98014285341822	37.0	34.0	40.0	26.0	41.0
42	34.88360006257392	37.0	34.0	40.0	26.0	41.0
43	34.7623204258819	36.0	34.0	40.0	26.0	41.0
44	34.54699265941536	36.0	34.0	40.0	25.0	41.0
45	34.39088435438646	36.0	34.0	40.0	24.0	41.0
46	34.32284173301319	36.0	34.0	40.0	24.0	41.0
47	34.21891924882852	35.0	33.0	40.0	23.0	41.0
48	34.15653553795796	35.0	33.0	40.0	23.0	41.0
49	34.034012718623565	36.0	33.0	40.0	24.0	41.0
50	33.911176437126244	35.0	33.0	40.0	24.0	41.0
51	33.543898924155194	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	2.0
12	11.0
13	3.0
14	28.0
15	30.0
16	128.0
17	305.0
18	639.0
19	1280.0
20	2438.0
21	3752.0
22	5780.0
23	8790.0
24	12909.0
25	18210.0
26	23631.0
27	28583.0
28	31721.0
29	36968.0
30	44023.0
31	53074.0
32	65482.0
33	85310.0
34	151594.0
35	241651.0
36	121057.0
37	144653.0
38	218030.0
39	387446.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.677810671223817	24.90643539598152	30.340648635580386	13.075105297214277
2	29.964375528856298	27.08692323554578	29.047928305455546	13.90077293014238
3	29.16715058746009	25.565002216159716	31.149072886767275	14.118774309612919
4	27.007876255330043	27.857957198489693	29.565111246477255	15.569055299703011
5	23.990699239869066	32.60699785020657	27.99211189354849	15.41019101637588
6	21.34458083768467	41.58985164766142	26.139129795852583	10.92643771880133
7	86.46755992519573	4.29988314794229	6.853918336292162	2.3786385905698255
8	85.67685310060891	3.8551698147195665	6.812972711607699	3.655004373063823
9	81.11897103822935	5.960225266116933	9.168323848485782	3.7524798471679373
10	47.409522613124885	28.108252883970408	13.625234355927102	10.856990146977608
11	38.68988222355482	22.50148731574469	23.53680128750584	15.271829173194659
12	37.63323623314474	21.663613027700812	24.91087956653338	15.792271172621064
13	22.55321746096833	37.107164951019314	24.705025586571256	15.634592001441098
14	15.046657865233787	41.30915783560598	26.009537782560365	17.634646516599865
15	13.667542859580802	28.50088053832534	41.775203187477636	16.056373414616225
16	16.209845437673767	24.070042498121598	40.71583143912908	19.004280625075552
17	15.946157984930116	25.699749467292087	26.326199748282182	32.02789279949562
18	20.244618998295806	27.92444198994551	30.53654767350634	21.29439133825234
19	24.10565511814383	30.374424331774513	24.88160729649847	20.63831325358319
20	27.051369871130902	27.165081381651145	25.3526301193882	20.430918627829755
21	20.384995532127206	31.397709415241966	27.265223358086377	20.952071694544454
22	21.328581823697977	26.81079210525692	26.161765437863384	25.69886063318172
23	18.854008404815346	32.13093830069139	24.61394971806182	24.40110357643144
24	20.62871384519117	27.395526438666888	33.52794849976654	18.447811216375406
25	17.82912341994923	29.094029167980167	30.256150139487698	22.820697272582905
26	17.276090836475856	36.146216766492614	25.02299117565495	21.55470122137658
27	18.8011524030519	35.34117008492514	26.58099885992212	19.27667865210085
28	15.324744430565465	31.401205496076095	34.0400947141628	19.23395535919564
29	16.383168089196282	27.857305386808758	33.04839286941723	22.711133654577733
30	19.146553338342407	32.857352791294645	28.507102377097944	19.488991493265008
31	25.952593143889207	31.805150971436426	21.977489979876797	20.26476590479757
32	26.26072230215145	32.52030689664163	22.356962889398226	18.862007911808696
33	23.90762287835298	32.67893415753933	22.14731655056518	21.266126413542512
34	17.950834437462817	30.80355344026205	25.414552229077437	25.831059893197693
35	18.945262040146858	29.27309961341642	26.76558007684267	25.016058269594048
36	24.862645502143867	29.149610927682083	25.61477692634054	20.372966643833507
37	18.63097029871937	33.44250191395612	28.06588512470935	19.860642662615163
38	17.804413831680893	33.86114277994127	23.911296726009184	24.423146662368662
39	18.270636950374612	32.64972114311177	26.299712491793098	22.779929414720517
40	22.177062865458957	28.18611475203899	25.46622311869372	24.170599263808334
41	16.549498579050535	26.350198269262222	27.87857814985032	29.221725001836923
42	21.669123799185115	28.212187219276558	24.25817905148364	25.860509930054683
43	22.042315614326583	29.16661728699387	25.45283135143079	23.338235747248763
44	19.34577069028042	32.49032355931842	25.834437462817107	22.329468287584053
45	17.994565075693114	35.534817409771485	21.917523305230375	24.553094209305026
46	22.90804003782878	32.04069201068497	24.203663892714168	20.84760405877208
47	19.545580598292016	29.67763764485033	26.675215275621532	24.10156648123612
48	21.21955150615903	26.475049833965787	28.888056676803327	23.417341983071857
49	20.0243066501383	25.830348825909393	29.241338607872464	24.90400591607984
50	17.871965224069154	29.368678908085073	29.516580904050947	23.242774963794822
51	17.069881322869584	31.506739732780915	24.702003550595993	26.72137539375351
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	919.0
1	1729.0
2	2539.0
3	8274.5
4	14010.0
5	10097.0
6	6184.0
7	6162.5
8	6141.0
9	7168.5
10	8196.0
11	9117.5
12	10039.0
13	10217.5
14	10396.0
15	9681.0
16	8966.0
17	8156.0
18	7346.0
19	6962.5
20	6579.0
21	6823.5
22	7068.0
23	7538.5
24	8009.0
25	8948.0
26	12292.0
27	14697.0
28	17203.0
29	19709.0
30	23484.0
31	27259.0
32	30314.5
33	33370.0
34	38878.5
35	44387.0
36	48433.0
37	52479.0
38	57174.0
39	61869.0
40	85592.5
41	109316.0
42	127140.0
43	144964.0
44	156993.0
45	169022.0
46	173251.5
47	177481.0
48	175983.5
49	174486.0
50	159599.5
51	144713.0
52	125726.0
53	106739.0
54	89963.5
55	73188.0
56	64574.0
57	55960.0
58	53511.0
59	51062.0
60	46543.0
61	42024.0
62	36503.0
63	30982.0
64	24520.0
65	18058.0
66	14769.0
67	11480.0
68	9448.0
69	7416.0
70	5676.5
71	3937.0
72	3452.0
73	2967.0
74	2277.0
75	1235.0
76	883.0
77	795.0
78	707.0
79	467.0
80	227.0
81	189.5
82	152.0
83	115.5
84	79.0
85	66.0
86	53.0
87	44.0
88	35.0
89	26.5
90	18.0
91	16.0
92	14.0
93	9.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1687604.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.472820367991407
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73368852437096	16.262158894892302
2	10.300507939856644	4.4236191338322195
3	4.053359207325775	2.6111116243755115
4	2.0547760278665974	1.764873461713374
5	1.1940563062397709	1.2819878286576971
6	0.7564221952363723	0.9745510752403411
7	0.5138494963895661	0.7723658552509202
8	0.3915645813039652	0.6726396733446249
9	0.2962161480143665	0.5724536522769763
>10	2.3611765894970613	11.224457139751406
>50	1.0503251847444943	16.84259200708049
>100	1.2859612131406484	40.08130571061703
>500	0.004467081938645516	0.6510627560554406
>1k	0.0033503114539841367	1.2557238018726757
>5k	0.0	0.0
>10k+	2.7919262116534474E-4	0.6090973850389757
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10160	0.6020369707585429	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4518	0.2677168340440056	No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT	2751	0.16301217584219996	No Hit
CCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	2131	0.12627369928016288	No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC	2088	0.12372570816376353	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.92556073581243E-5	0.0	0.0	0.2018838542691295	0.0
2	5.92556073581243E-5	0.0	0.0	0.6766990360297795	0.0
3	5.92556073581243E-5	0.0	0.0	0.9676440681581698	0.0
4	5.92556073581243E-5	0.0	0.0	1.465924470432637	0.0
5	5.92556073581243E-5	0.0	0.0	2.503371644058677	0.0
6	5.92556073581243E-5	0.0	0.0	3.5008212827179834	0.0
7	5.92556073581243E-5	0.0	0.0	4.11601299831003	0.0
8	5.92556073581243E-5	0.0	0.0	5.589285164055075	0.0
9	5.92556073581243E-5	0.0	0.0	6.057759995828405	0.0
10	5.92556073581243E-5	0.0	0.0	6.854096103114237	0.0
11	5.92556073581243E-5	0.0	0.0	8.03743058205598	0.0
12	5.92556073581243E-5	0.0	0.0	8.937997302684753	0.0
13	5.92556073581243E-5	0.0	0.0	9.286598040772597	0.0
14	5.92556073581243E-5	0.0	0.0	9.428218942358516	0.0
15	5.92556073581243E-5	0.0	0.0	9.648649801730738	0.0
16	5.92556073581243E-5	0.0	0.0	10.181713245524424	0.0
17	5.92556073581243E-5	0.0	0.0	10.794890270466293	0.0
18	5.92556073581243E-5	0.0	0.0	11.571672027323945	0.0
19	5.92556073581243E-5	0.0	0.0	11.926376092969678	0.0
20	5.92556073581243E-5	0.0	0.0	12.302826966515841	0.0
21	5.92556073581243E-5	0.0	0.0	12.76282824643696	0.0
22	5.92556073581243E-5	0.0	0.0	13.19657929229843	0.0
23	5.92556073581243E-5	0.0	0.0	13.64775148672319	0.0
24	5.92556073581243E-5	0.0	0.0	13.98159757857886	0.0
25	1.185112147162486E-4	0.0	0.0	14.259802655125254	0.0
26	1.185112147162486E-4	0.0	0.0	14.520468071893644	0.0
27	1.185112147162486E-4	0.0	0.0	14.79002182976575	0.0
28	1.185112147162486E-4	0.0	0.0	15.070774897428544	0.0
29	1.185112147162486E-4	0.0	0.0	15.36503824356899	0.0
30	1.185112147162486E-4	0.0	0.0	15.744629664305133	0.0
31	1.185112147162486E-4	0.0	0.0	16.07616478747384	0.0
32	1.185112147162486E-4	0.0	0.0	16.365628429418276	0.0
33	1.185112147162486E-4	0.0	0.0	16.642352115780717	0.0
34	1.777668220743729E-4	0.0	0.0	16.95468842216539	0.0
35	1.777668220743729E-4	0.0	0.0	17.33410207607946	0.0
36	1.777668220743729E-4	0.0	0.0	17.669192535689653	0.0
37	1.777668220743729E-4	0.0	0.0	17.98324725468771	0.0
38	1.777668220743729E-4	0.0	0.0	18.27650325550307	0.0
39	1.777668220743729E-4	0.0	0.0	18.590854252537916	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	60	0.0	45.000004	29
AATCCGG	25	3.8915845E-5	45.000004	44
CTCCGAT	35	1.2123746E-7	45.000004	45
TACGCTT	30	2.1661072E-6	45.000004	28
GTTCGCC	25	3.8915845E-5	45.000004	27
TACGACG	30	2.1661072E-6	45.000004	1
TTGACGA	25	3.8915845E-5	45.000004	32
GGACGTA	25	3.8915845E-5	45.000004	9
CGCAATC	25	3.8915845E-5	45.000004	18
TAGGACG	60	0.0	45.000004	1
CGCCCTA	25	3.8915845E-5	45.000004	17
CTTACGT	30	2.1661072E-6	45.000004	29
AAGTACG	45	3.8562575E-10	45.0	1
GTTTACG	40	6.8175723E-9	45.0	1
TTTCGCG	75	0.0	45.0	1
CACGCTA	20	7.034227E-4	45.0	19
CATTACG	20	7.034227E-4	45.0	30
TACGATA	20	7.034227E-4	45.0	37
CGGATAG	20	7.034227E-4	45.0	14
CACTACG	40	6.8175723E-9	45.0	1
>>END_MODULE