FastQCFastQC Report
Thu 26 May 2016
SRR1547862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547862_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1604329
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153900.9592795492695076No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30220.18836535398911322No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT18160.11319374018670735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCGC207.034122E-445.00000423
CGAATTA302.1660417E-645.00000423
GTCCGAG302.1660417E-645.0000041
TCGAATA207.034122E-445.00000425
GACCGTA253.8914986E-545.09
TAACGCG253.8914986E-545.01
GTATCGT351.2123382E-745.025
CACGTTA253.8914986E-545.036
TATACGA253.8914986E-545.030
TATCGTG253.8914986E-545.01
CGTTTTT60450.043.9205971
CGACGGT2600.043.2692328
CCGCTCG1250.043.19999719
CGGTCTA2600.042.40384331
TCGATTG750.042.01
ACGCCGA1300.041.5384612
ACGTAAG603.6379788E-1241.2500041
TAGGGCG4650.041.129035
CGATACT556.184564E-1140.90909212
TCGCGTG501.0822987E-940.51
Produced by FastQC (version 0.11.5)