##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547862_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1604329
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413248155459385	31.0	31.0	33.0	30.0	34.0
2	31.8008357388042	31.0	31.0	34.0	30.0	34.0
3	31.77745026113721	31.0	31.0	34.0	30.0	34.0
4	35.59415556285525	37.0	35.0	37.0	33.0	37.0
5	35.531978789886615	37.0	35.0	37.0	33.0	37.0
6	35.585919097641444	37.0	35.0	37.0	33.0	37.0
7	35.978415275171116	37.0	35.0	37.0	35.0	37.0
8	36.015967423140765	37.0	35.0	37.0	35.0	37.0
9	37.78633808900793	39.0	38.0	39.0	35.0	39.0
10	37.25383322248741	39.0	37.0	39.0	34.0	39.0
11	36.957738094867075	39.0	37.0	39.0	33.0	39.0
12	36.29896049999719	38.0	35.0	39.0	32.0	39.0
13	36.055777836092226	38.0	35.0	39.0	32.0	39.0
14	37.10318394793088	39.0	35.0	41.0	32.0	41.0
15	37.31164617731151	39.0	35.0	41.0	32.0	41.0
16	37.426885632560406	39.0	35.0	41.0	33.0	41.0
17	37.35343685740269	39.0	35.0	41.0	33.0	41.0
18	37.252288028203694	39.0	35.0	41.0	32.0	41.0
19	37.13690645746602	38.0	35.0	41.0	32.0	41.0
20	36.97114868583688	38.0	35.0	41.0	32.0	41.0
21	36.845745479885984	38.0	35.0	41.0	32.0	41.0
22	36.71853341802087	38.0	35.0	41.0	31.0	41.0
23	36.63352903301006	38.0	35.0	40.0	31.0	41.0
24	36.63563520948633	38.0	35.0	41.0	31.0	41.0
25	36.599245541282365	38.0	35.0	40.0	31.0	41.0
26	36.47633745946125	38.0	35.0	40.0	31.0	41.0
27	36.375296463505926	38.0	35.0	40.0	31.0	41.0
28	36.31881054322399	38.0	35.0	40.0	31.0	41.0
29	36.338252316077316	38.0	35.0	40.0	31.0	41.0
30	36.24739003034914	38.0	35.0	40.0	31.0	41.0
31	36.085043030450734	38.0	35.0	40.0	30.0	41.0
32	35.87940628137994	38.0	35.0	40.0	30.0	41.0
33	35.73711875806023	38.0	35.0	40.0	30.0	41.0
34	35.59999538747975	38.0	35.0	40.0	29.0	41.0
35	35.452410945635215	37.0	35.0	40.0	29.0	41.0
36	35.32922798253974	37.0	35.0	40.0	27.0	41.0
37	35.18291136045038	37.0	34.0	40.0	27.0	41.0
38	35.10527641150911	37.0	34.0	40.0	27.0	41.0
39	35.0516066218338	37.0	34.0	40.0	26.0	41.0
40	34.89771861008559	37.0	34.0	40.0	25.0	41.0
41	34.87644990522517	37.0	34.0	40.0	25.0	41.0
42	34.79821283539723	37.0	34.0	40.0	25.0	41.0
43	34.714199518926605	37.0	34.0	40.0	25.0	41.0
44	34.545530249718105	36.0	34.0	40.0	24.0	41.0
45	34.386953673467225	36.0	34.0	40.0	24.0	41.0
46	34.33104244827588	36.0	34.0	40.0	23.0	41.0
47	34.26067284204175	36.0	33.0	40.0	23.0	41.0
48	34.175232137547845	36.0	33.0	40.0	23.0	41.0
49	34.04887276861542	36.0	33.0	40.0	23.0	41.0
50	33.931496594526436	35.0	33.0	40.0	23.0	41.0
51	33.57403749480312	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	10.0
15	30.0
16	90.0
17	241.0
18	529.0
19	1196.0
20	2206.0
21	3669.0
22	5614.0
23	8738.0
24	13004.0
25	18996.0
26	24953.0
27	27783.0
28	30040.0
29	33998.0
30	39815.0
31	48368.0
32	59765.0
33	77541.0
34	136765.0
35	224169.0
36	120008.0
37	139785.0
38	210318.0
39	376626.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.44325696287981	24.602622030768003	30.48246338500395	14.471657621348239
2	30.996759392867673	25.688621224200276	30.321523827095316	12.993095555836739
3	28.085012488086917	24.88423509143075	33.919351953371155	13.111400467111173
4	25.291258837806957	27.305995216691837	33.12525049413181	14.277495451369388
5	22.64385920842919	31.35211044617407	31.939396470424708	14.06463387497203
6	19.928393739688058	40.408793956850495	28.765421556301728	10.897390747159715
7	86.69499834510253	3.6532407006293592	7.699667586885234	1.9520933673828746
8	87.34648566472339	2.6582452851005	7.2106781090412255	2.784590941134892
9	83.46430189817674	4.533733417522217	9.185023770062125	2.8169409142389124
10	44.70192834512123	28.58621891145769	14.672302252218842	12.03955049120224
11	36.34478962856122	24.37286865723926	24.980412371776612	14.301929342422905
12	34.47771622902784	22.91624722859214	26.57752867398146	16.028507868398563
13	20.908491961436837	36.048092380054214	27.706349508112115	15.337066150396833
14	15.329025405636873	39.32379206509388	28.124468235629973	17.22271429363927
15	13.548717251885368	27.89876640015857	42.77040432479872	15.782112023157344
16	15.75150732798572	24.299816309497615	41.657477986123794	18.291198376392874
17	16.306381047777606	24.991320358854075	28.530868668458904	30.171429924909415
18	19.613059416117267	28.008220259061577	32.42626668220795	19.952453642613204
19	23.580574807287032	29.49264147191754	27.053802555460884	19.87298116533454
20	26.206844107411886	27.535062945318572	25.884777997530435	20.373314949739115
21	20.53132493397551	30.832017622320606	29.020107471721822	19.616549971982057
22	21.501512470322485	26.657437470743222	27.134396997124654	24.706653061809643
23	17.780891575231763	31.67835275682232	26.825171146317246	23.715584521628667
24	18.609773930409535	27.88860638933785	35.518026539444215	17.983593140808402
25	16.898528917697057	29.482606123806278	32.181678446253855	21.43718651224281
26	16.353191895178607	35.029785037856946	27.498661434157206	21.118361632807236
27	17.40553215705756	33.91417845092871	29.057069965075744	19.62321942693799
28	15.335258541109711	30.58749171772124	36.02584008641619	18.05140965475286
29	15.815334635227563	27.02936866440736	34.82589917654047	22.3293975238246
30	18.681081000218782	30.778287994544762	31.092625016439897	19.448005988796563
31	24.919265312788088	29.088671961923023	26.054880264584135	19.937182460704754
32	24.65784761105733	28.997044870472326	26.71066844768124	19.634439070789096
33	23.287056457871174	29.25067115286204	27.172107466735312	20.290164922531474
34	18.389370260090043	28.122847620407036	29.93631605487403	23.55146606462889
35	18.581226169943946	27.145554309621033	30.6164134663152	23.656806054119823
36	25.594563209915172	27.124423980368118	28.30666278550098	18.97435002421573
37	19.0177949784614	30.687533542060265	31.43445016577024	18.8602213137081
38	19.057936370906468	31.743925341996558	26.905890250690472	22.292248036406498
39	20.17397927731781	31.23785707295698	27.945016265367016	20.643147384358194
40	21.86477960567938	27.91247929819881	27.121245081276967	23.101496014844834
41	16.93742368304755	26.384924787870816	29.253164407051173	27.424487122030456
42	20.832697034087147	28.100595326769014	26.273476325616503	24.793231313527336
43	20.83300869086079	28.885160088734917	27.65766872006926	22.624162500335032
44	19.99677123582507	31.55057347962918	27.2834935976349	21.16916168691085
45	18.796394006466254	33.53676209804847	24.487994669422545	23.178849226062734
46	22.302221053163034	31.40652571885193	26.5130157218376	19.77823750614743
47	20.18463793897636	28.219897539719096	28.899994951160267	22.695469570144276
48	21.225509231585292	26.026083178699633	30.690276121668308	22.058131468046767
49	20.856756937012296	25.312326835705147	30.246539207357092	23.584377019925466
50	19.21476205940303	29.341051617218167	29.8162658656672	21.627920457711607
51	18.397411004850003	30.400248328117236	26.535143352766173	24.667197314266588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	962.0
1	1460.5
2	1959.0
3	10965.5
4	19972.0
5	14138.5
6	8305.0
7	8303.0
8	8301.0
9	9840.5
10	11380.0
11	12675.5
12	13971.0
13	13986.0
14	14001.0
15	13340.0
16	12679.0
17	11842.0
18	11005.0
19	10119.5
20	9234.0
21	9207.0
22	9180.0
23	9726.5
24	10273.0
25	10814.5
26	13404.5
27	15453.0
28	17250.0
29	19047.0
30	21899.0
31	24751.0
32	28796.0
33	32841.0
34	38192.0
35	43543.0
36	46091.0
37	48639.0
38	56060.5
39	63482.0
40	80050.0
41	96618.0
42	117277.0
43	137936.0
44	143951.0
45	149966.0
46	156547.0
47	163128.0
48	165328.5
49	167529.0
50	153894.0
51	140259.0
52	122624.5
53	104990.0
54	86992.5
55	68995.0
56	61527.5
57	54060.0
58	47990.0
59	41920.0
60	36985.0
61	32050.0
62	26906.0
63	21762.0
64	18096.0
65	14430.0
66	11547.0
67	8664.0
68	6853.5
69	5043.0
70	3928.0
71	2813.0
72	2259.5
73	1706.0
74	1172.0
75	661.5
76	685.0
77	531.0
78	377.0
79	255.0
80	133.0
81	132.5
82	132.0
83	109.0
84	86.0
85	61.5
86	37.0
87	26.5
88	16.0
89	15.5
90	15.0
91	10.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1604329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.782175046589213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.1634531580812	18.874960283162665
2	9.661759159484182	4.788788134966474
3	3.6209828559108788	2.692074929276458
4	1.7955623127618763	1.7799175816769357
5	1.0835515212560416	1.3426381735882627
6	0.6955987027041686	1.0343069287557045
7	0.5106079225298799	0.8857782441417524
8	0.3967877809797756	0.7866611395670801
9	0.33518464132943593	0.7475944008918872
>10	3.606963424246727	21.364505016453755
>50	1.7428027833157704	31.064070321719083
>100	0.38088981117650844	12.337691556297658
>500	0.004073687807671072	0.7042862327910724
>1k	0.001527632927876652	0.6256676477652995
>5k	0.0	0.0
>10k+	2.54605487979442E-4	0.9710594089459419
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15390	0.9592795492695076	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3022	0.18836535398911322	No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT	1816	0.11319374018670735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.233135472836307E-5	0.0	0.0	0.14386076671306197	0.0
2	6.233135472836307E-5	0.0	0.0	0.452026984490089	0.0
3	6.233135472836307E-5	0.0	0.0	0.6895094460051523	0.0
4	6.233135472836307E-5	0.0	0.0	1.0938529441280436	0.0
5	6.233135472836307E-5	0.0	0.0	1.8908839770396222	0.0
6	6.233135472836307E-5	0.0	0.0	2.8886843035312584	0.0
7	6.233135472836307E-5	0.0	0.0	3.4957916985855144	0.0
8	6.233135472836307E-5	0.0	0.0	4.774955760320982	0.0
9	6.233135472836307E-5	0.0	0.0	5.343355384088924	0.0
10	6.233135472836307E-5	0.0	0.0	6.259189979112763	0.0
11	6.233135472836307E-5	0.0	0.0	7.363389928125715	0.0
12	6.233135472836307E-5	0.0	0.0	8.1457107613214	0.0
13	6.233135472836307E-5	0.0	0.0	8.483172715820757	0.0
14	6.233135472836307E-5	0.0	0.0	8.61213628875374	0.0
15	6.233135472836307E-5	0.0	0.0	8.793645193722734	0.0
16	6.233135472836307E-5	0.0	0.0	9.20010795790639	0.0
17	6.233135472836307E-5	0.0	0.0	9.670647354750802	0.0
18	6.233135472836307E-5	0.0	0.0	10.282055613281315	0.0
19	6.233135472836307E-5	0.0	0.0	10.588538884480677	0.0
20	6.233135472836307E-5	0.0	0.0	10.917399112027521	0.0
21	6.233135472836307E-5	0.0	0.0	11.32903537865363	0.0
22	6.233135472836307E-5	0.0	0.0	11.742728579985776	0.0
23	6.233135472836307E-5	0.0	0.0	12.200303055046689	0.0
24	6.233135472836307E-5	0.0	0.0	12.54368648824524	0.0
25	6.233135472836307E-5	0.0	0.0	12.813830579637967	0.0
26	6.233135472836307E-5	0.0	0.0	13.061348389264296	1.2466270945672613E-4
27	6.233135472836307E-5	0.0	0.0	13.307432577731873	1.2466270945672613E-4
28	6.233135472836307E-5	0.0	0.0	13.562243155861422	1.8699406418508922E-4
29	6.233135472836307E-5	0.0	0.0	13.84298357755797	1.8699406418508922E-4
30	6.233135472836307E-5	0.0	0.0	14.227256379458328	1.8699406418508922E-4
31	6.233135472836307E-5	0.0	0.0	14.54820052495467	1.8699406418508922E-4
32	6.233135472836307E-5	0.0	0.0	14.81971590615142	1.8699406418508922E-4
33	6.233135472836307E-5	0.0	0.0	15.103572895584385	1.8699406418508922E-4
34	6.233135472836307E-5	0.0	0.0	15.391855411203064	1.8699406418508922E-4
35	6.233135472836307E-5	0.0	0.0	15.743965234063587	1.8699406418508922E-4
36	6.233135472836307E-5	0.0	0.0	16.024954981179047	1.8699406418508922E-4
37	6.233135472836307E-5	0.0	0.0	16.310183260416036	1.8699406418508922E-4
38	6.233135472836307E-5	0.0	0.0	16.59042503127476	1.8699406418508922E-4
39	6.233135472836307E-5	0.0	0.0	16.884192706109534	1.8699406418508922E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCGC	20	7.034122E-4	45.000004	23
CGAATTA	30	2.1660417E-6	45.000004	23
GTCCGAG	30	2.1660417E-6	45.000004	1
TCGAATA	20	7.034122E-4	45.000004	25
GACCGTA	25	3.8914986E-5	45.0	9
TAACGCG	25	3.8914986E-5	45.0	1
GTATCGT	35	1.2123382E-7	45.0	25
CACGTTA	25	3.8914986E-5	45.0	36
TATACGA	25	3.8914986E-5	45.0	30
TATCGTG	25	3.8914986E-5	45.0	1
CGTTTTT	6045	0.0	43.920597	1
CGACGGT	260	0.0	43.26923	28
CCGCTCG	125	0.0	43.199997	19
CGGTCTA	260	0.0	42.403843	31
TCGATTG	75	0.0	42.0	1
ACGCCGA	130	0.0	41.53846	12
ACGTAAG	60	3.6379788E-12	41.250004	1
TAGGGCG	465	0.0	41.12903	5
CGATACT	55	6.184564E-11	40.909092	12
TCGCGTG	50	1.0822987E-9	40.5	1
>>END_MODULE