FastQCFastQC Report
Thu 26 May 2016
SRR1547861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547861_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2182328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72490.332168216693366No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC27970.12816588523814934No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGT253.8919625E-545.08
GCGATAC406.8193913E-945.09
GACGATA603.6379788E-1241.2500049
CGTTTTT38300.041.063971
CGGTCTA3400.040.3676531
CGCGTAA1300.039.80769331
TACGGGA8550.039.736844
TCGTTAG850.039.7058831
ACGTAAG850.039.7058831
TACAACG403.4601362E-739.3751
ACGGTCT3500.039.21428730
TTAGGGA26650.039.005634
TAATGCG750.039.01
TAGGGAC22450.038.9866375
ACGGGAT9250.038.918925
AGTACGG2200.038.8636362
CGTTAGG2900.038.7931022
TCACGAC3700.038.3108125
ATCGTAG601.5643309E-1037.5000041
CGTAGCA301.14025795E-437.50000414
Produced by FastQC (version 0.11.5)