##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547861_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2182328
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.461225810235675	31.0	31.0	33.0	30.0	34.0
2	31.87568917229674	31.0	31.0	34.0	30.0	34.0
3	31.990983940086	33.0	31.0	34.0	30.0	34.0
4	35.74848785333827	37.0	35.0	37.0	35.0	37.0
5	35.649033509170025	37.0	35.0	37.0	33.0	37.0
6	35.68061263018208	37.0	35.0	37.0	33.0	37.0
7	35.995708252838256	37.0	35.0	37.0	35.0	37.0
8	36.014968419046085	37.0	35.0	37.0	35.0	37.0
9	37.75650498000301	39.0	38.0	39.0	35.0	39.0
10	37.28903996099578	39.0	37.0	39.0	34.0	39.0
11	37.0020171120015	39.0	37.0	39.0	33.0	39.0
12	36.42536593949214	38.0	35.0	39.0	32.0	39.0
13	36.215328768177834	39.0	35.0	39.0	32.0	39.0
14	37.19553201901822	39.0	35.0	41.0	32.0	41.0
15	37.424603450993615	39.0	35.0	41.0	32.0	41.0
16	37.53927411461522	39.0	35.0	41.0	33.0	41.0
17	37.46754658328171	39.0	35.0	41.0	33.0	41.0
18	37.3987814847264	39.0	35.0	41.0	32.0	41.0
19	37.332457357464136	39.0	35.0	41.0	32.0	41.0
20	37.19677060460206	39.0	35.0	41.0	32.0	41.0
21	37.081627509705235	39.0	35.0	41.0	32.0	41.0
22	36.997256599374616	39.0	35.0	41.0	32.0	41.0
23	36.92078734269092	39.0	35.0	41.0	32.0	41.0
24	36.9630422191348	39.0	35.0	41.0	32.0	41.0
25	36.937481441836425	39.0	35.0	41.0	32.0	41.0
26	36.82308617219776	39.0	35.0	41.0	31.0	41.0
27	36.73292282370019	38.0	35.0	41.0	31.0	41.0
28	36.66458983250914	38.0	35.0	41.0	31.0	41.0
29	36.678136375466934	38.0	35.0	41.0	31.0	41.0
30	36.58688657250423	38.0	35.0	41.0	31.0	41.0
31	36.441985805983336	38.0	35.0	41.0	31.0	41.0
32	36.34173735570455	38.0	35.0	41.0	30.0	41.0
33	36.25549550754974	38.0	35.0	41.0	30.0	41.0
34	36.14103333687695	38.0	35.0	41.0	30.0	41.0
35	36.07361588175563	38.0	35.0	41.0	30.0	41.0
36	35.97204224112966	38.0	35.0	40.0	30.0	41.0
37	35.87535375067359	38.0	35.0	40.0	29.0	41.0
38	35.78255789230583	38.0	35.0	40.0	29.0	41.0
39	35.69846695822076	38.0	35.0	40.0	29.0	41.0
40	35.52546867381989	38.0	35.0	40.0	28.0	41.0
41	35.46744210769417	38.0	34.0	40.0	27.0	41.0
42	35.40948473373388	38.0	34.0	40.0	27.0	41.0
43	35.35995780652588	38.0	34.0	40.0	27.0	41.0
44	35.195037134656204	37.0	34.0	40.0	27.0	41.0
45	35.056647305079714	37.0	34.0	40.0	27.0	41.0
46	35.01712849764105	37.0	34.0	40.0	27.0	41.0
47	34.94877396981572	37.0	34.0	40.0	26.0	41.0
48	34.85460801492718	37.0	34.0	40.0	26.0	41.0
49	34.72964421480181	37.0	34.0	40.0	26.0	41.0
50	34.63110861428713	36.0	34.0	40.0	26.0	41.0
51	34.267721900649214	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	1.0
11	5.0
12	11.0
13	9.0
14	19.0
15	56.0
16	167.0
17	331.0
18	795.0
19	1542.0
20	2758.0
21	4328.0
22	6719.0
23	9695.0
24	14226.0
25	20434.0
26	26705.0
27	32468.0
28	36468.0
29	42590.0
30	51010.0
31	61893.0
32	76890.0
33	100405.0
34	175182.0
35	255850.0
36	152592.0
37	202942.0
38	321746.0
39	584407.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.176026701760684	24.089825177516854	29.598621288825512	12.135526831896946
2	29.902471122581026	26.23670685616461	30.692865600404705	13.167956420849661
3	29.585974244018313	25.86137372567277	30.55599341620508	13.996658614103838
4	26.14698615423529	27.496645783768525	31.07947109692035	15.276896965075828
5	23.738273989977678	32.239333409093405	29.219255767235726	14.803136833693193
6	21.578057927131027	41.58815723392634	25.832688761726008	11.001096077216625
7	88.92091381313901	3.5882323830331644	5.610751454410153	1.8801023494176858
8	89.42180093918054	2.7715815404467157	5.196514914348347	2.6101026060243924
9	84.57976986044261	5.198943513532337	7.127892782386517	3.093393843638536
10	44.73704227778776	32.063649460576045	12.517229307418502	10.682078954217697
11	35.4099383777324	22.28395548240228	25.409425164319938	16.89668097554538
12	33.73823733187679	20.89612560531689	27.819786943117624	17.5458501196887
13	24.54910535904777	30.521855559750872	27.986489656916834	16.942549424284525
14	17.762728609081677	34.10078594968309	29.683164034004054	18.453321407231176
15	16.55475253948994	26.390991638287186	40.055436213071545	16.998819609151326
16	20.497789516516306	23.049743210003264	37.47585147603843	18.976615797441998
17	20.382728902346486	23.72383986275207	27.89649401923084	27.996937215670602
18	23.065231257629467	24.92902075215091	31.50557569714543	20.500172293074183
19	26.01799546172711	27.25804736959797	26.525801804311726	20.198155364363192
20	27.350059202832938	27.021190215219708	26.252057435912473	19.376693146034878
21	24.080019135528666	27.045751142816293	29.440441583483324	19.433788138171714
22	22.886981242049774	23.692726299621324	28.660952890674547	24.759339567654358
23	20.220287692775788	28.10342899875729	27.9846109292462	23.691672379220723
24	20.832661268150343	26.374037266625365	32.52503748290816	20.26826398231613
25	20.55561767067095	26.51828689362919	30.541101062718347	22.384994372981513
26	19.307867561613104	30.577438405225976	27.036907375976483	23.077786657184436
27	19.79798637051809	29.733843858485066	29.624969298840504	20.84320047215634
28	17.384233717388035	27.75412311989765	34.27509521941706	20.58654794329725
29	18.691186659383924	25.6403253773035	33.328308118669604	22.340179844642968
30	21.062599205985535	27.32224486878233	31.365862510126803	20.24929341510534
31	25.698795048223733	26.139425420926642	26.932385965812657	21.22939356503697
32	26.08315523605984	26.482866003643817	27.079614063513823	20.35436469678252
33	24.66916063946391	26.664827651938666	27.20878804652646	21.457223662070962
34	19.91263458105289	26.763208830203343	30.115867092389408	23.20828949635435
35	19.881612663174373	26.841565520856626	29.906503513678974	23.370318302290034
36	26.020240770406648	24.767862576111384	28.98354417851029	20.228352474971683
37	20.610742289884932	28.971172069459772	29.226770677918264	21.19131496273704
38	20.912117701830336	28.951101759222265	27.00382343992287	23.13295709902453
39	20.563774098119072	27.821161621901012	27.733961164407916	23.881103115571996
40	22.64627498707802	25.7996048256724	28.480595034293653	23.073525152955927
41	19.632291754493366	24.795127038648634	28.989180361522195	26.58340084533581
42	21.91861168440308	25.341286919289857	28.063884072421747	24.676217323885318
43	22.198862865710378	24.982266643694256	28.442149850984823	24.376720639610543
44	21.015126965332435	27.395698538441515	28.417726391266573	23.171448104959474
45	19.647367398484555	29.8981637957264	26.131039880347956	24.32342892544109
46	22.41083833410926	27.437442950830487	27.965090490522048	22.1866282245382
47	20.81767726941138	26.610802775751402	28.96901840603246	23.602501548804764
48	21.519496610958573	24.743026712758116	30.833311949441146	22.90416472684216
49	21.73183866036636	23.37742080933755	30.819061112719993	24.071679417576092
50	19.73209343416755	27.70073976047597	29.41340623407664	23.153760571279843
51	19.338247962726044	28.50547672027303	27.21607384407843	24.940201472922492
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	884.0
1	1688.5
2	2493.0
3	7579.5
4	12666.0
5	9477.5
6	6289.0
7	6032.5
8	5776.0
9	6128.5
10	6481.0
11	6436.5
12	6392.0
13	6286.0
14	6180.0
15	6005.0
16	5830.0
17	5839.0
18	5848.0
19	6174.5
20	6501.0
21	7618.0
22	8735.0
23	10236.0
24	11737.0
25	13201.0
26	18405.5
27	22146.0
28	27209.5
29	32273.0
30	35350.0
31	38427.0
32	43112.0
33	47797.0
34	56099.0
35	64401.0
36	69533.0
37	74665.0
38	83888.5
39	93112.0
40	111940.5
41	130769.0
42	150747.0
43	170725.0
44	179445.0
45	188165.0
46	193430.0
47	198695.0
48	198797.0
49	198899.0
50	185862.5
51	172826.0
52	158263.5
53	143701.0
54	127407.5
55	111114.0
56	103128.0
57	95142.0
58	88934.0
59	82726.0
60	74084.0
61	65442.0
62	58499.5
63	51557.0
64	44746.0
65	37935.0
66	30963.5
67	23992.0
68	19604.5
69	15217.0
70	12747.0
71	10277.0
72	7843.5
73	5410.0
74	4294.5
75	2370.5
76	1562.0
77	1175.5
78	789.0
79	600.0
80	411.0
81	316.0
82	221.0
83	182.0
84	143.0
85	103.5
86	64.0
87	43.0
88	22.0
89	21.5
90	21.0
91	16.5
92	12.0
93	12.0
94	12.0
95	7.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2182328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.69021011627462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75671482312026	15.113558427022088
2	8.878427097498877	3.496361901035582
3	3.1223337040874544	1.84438220059824
4	1.4587308971401913	1.1489087147116859
5	0.9082261225600865	0.8941581593148746
6	0.6205097757125021	0.7330780717788963
7	0.4627140990856239	0.6377656484331053
8	0.35271785799089617	0.5556070988482448
9	0.30437734784501796	0.5393928540332531
>10	3.835199806128438	19.55145264781734
>50	2.6380389953792514	37.81288984516098
>100	0.6551480460752843	16.128963145644743
>500	0.004022216048088254	0.5147888142794115
>1k	0.0026026103840571056	0.6909816917097041
>5k	2.366009440051914E-4	0.3377107796118963
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7249	0.332168216693366	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2797	0.12816588523814934	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.582262611303159E-5	0.0	0.0	0.07996048256724012	0.0
2	4.582262611303159E-5	0.0	0.0	0.30494957678222523	0.0
3	4.582262611303159E-5	0.0	0.0	0.4531399496317694	0.0
4	4.582262611303159E-5	0.0	0.0	0.789111444292517	0.0
5	4.582262611303159E-5	0.0	0.0	1.385309632649171	0.0
6	4.582262611303159E-5	0.0	0.0	2.004602424566793	0.0
7	4.582262611303159E-5	0.0	0.0	2.332829895414438	0.0
8	4.582262611303159E-5	0.0	0.0	3.152367563446008	0.0
9	4.582262611303159E-5	0.0	0.0	3.4160309541003917	0.0
10	4.582262611303159E-5	0.0	0.0	3.9705763753203	0.0
11	4.582262611303159E-5	0.0	0.0	4.616537935635707	0.0
12	4.582262611303159E-5	0.0	0.0	5.159169474066227	0.0
13	4.582262611303159E-5	0.0	0.0	5.3576730903878795	0.0
14	4.582262611303159E-5	0.0	0.0	5.43809179921625	0.0
15	4.582262611303159E-5	0.0	0.0	5.565936926071608	0.0
16	4.582262611303159E-5	0.0	0.0	5.85677313401102	0.0
17	4.582262611303159E-5	0.0	0.0	6.196502084013035	0.0
18	4.582262611303159E-5	0.0	0.0	6.633100065618001	0.0
19	4.582262611303159E-5	0.0	0.0	6.869544816361244	0.0
20	4.582262611303159E-5	0.0	0.0	7.1094262640629635	0.0
21	4.582262611303159E-5	0.0	0.0	7.41620874588971	0.0
22	4.582262611303159E-5	0.0	0.0	7.751859482167667	0.0
23	4.582262611303159E-5	0.0	0.0	8.087189460062833	0.0
24	4.582262611303159E-5	0.0	0.0	8.344895909322522	0.0
25	4.582262611303159E-5	0.0	0.0	8.558383524383135	0.0
26	4.582262611303159E-5	0.0	0.0	8.762523323716692	0.0
27	4.582262611303159E-5	0.0	0.0	8.97940181310967	0.0
28	4.582262611303159E-5	0.0	0.0	9.202878760662925	0.0
29	4.582262611303159E-5	0.0	0.0	9.453207767118416	0.0
30	4.582262611303159E-5	0.0	0.0	9.75421659805492	0.0
31	4.582262611303159E-5	0.0	0.0	10.01054836853122	0.0
32	4.582262611303159E-5	0.0	0.0	10.246947296648349	0.0
33	4.582262611303159E-5	0.0	0.0	10.494114541902043	0.0
34	4.582262611303159E-5	0.0	0.0	10.7576862873042	0.0
35	4.582262611303159E-5	0.0	0.0	11.0583285372318	0.0
36	4.582262611303159E-5	0.0	0.0	11.307557800660579	0.0
37	4.582262611303159E-5	0.0	0.0	11.562744005484053	0.0
38	4.582262611303159E-5	0.0	0.0	11.861736640871584	0.0
39	4.582262611303159E-5	0.0	0.0	12.298884493989904	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGT	25	3.8919625E-5	45.0	8
GCGATAC	40	6.8193913E-9	45.0	9
GACGATA	60	3.6379788E-12	41.250004	9
CGTTTTT	3830	0.0	41.06397	1
CGGTCTA	340	0.0	40.36765	31
CGCGTAA	130	0.0	39.807693	31
TACGGGA	855	0.0	39.73684	4
TCGTTAG	85	0.0	39.705883	1
ACGTAAG	85	0.0	39.705883	1
TACAACG	40	3.4601362E-7	39.375	1
ACGGTCT	350	0.0	39.214287	30
TTAGGGA	2665	0.0	39.00563	4
TAATGCG	75	0.0	39.0	1
TAGGGAC	2245	0.0	38.986637	5
ACGGGAT	925	0.0	38.91892	5
AGTACGG	220	0.0	38.863636	2
CGTTAGG	290	0.0	38.793102	2
TCACGAC	370	0.0	38.31081	25
ATCGTAG	60	1.5643309E-10	37.500004	1
CGTAGCA	30	1.14025795E-4	37.500004	14
>>END_MODULE