FastQCFastQC Report
Thu 26 May 2016
SRR1547860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547860_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1619853
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131700.8130367385188656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.0341444E-445.01
ACGGCGT207.0341444E-445.031
CGTTTTT59200.042.1114851
TAATACG750.042.01
TACGGGA7250.041.275864
CTCCGAT451.9292202E-840.030
CGTAAGG2500.039.62
AGGGCGA11550.039.5454566
GCGAACG403.4593904E-739.3751
ACGTGCG403.4593904E-739.3751
TTGCGAG1500.039.01
CTAGCGG2150.038.7209322
GGGCGAT22500.038.77
CGTTAGG1700.038.382352
GACCGAT6850.038.102199
TAGGGCG4800.037.9687545
TGTAGCG1900.037.8947371
TAGGGAT27100.037.7767525
TCACGAC1550.037.74193625
ATAGGGA22050.037.653064
Produced by FastQC (version 0.11.5)