##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547860_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1619853
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50061270991874	31.0	31.0	33.0	30.0	34.0
2	31.90750333517918	31.0	31.0	34.0	30.0	34.0
3	31.900373675882935	33.0	31.0	34.0	30.0	34.0
4	35.70492445919476	37.0	35.0	37.0	33.0	37.0
5	35.6588671935046	37.0	35.0	37.0	33.0	37.0
6	35.713835144300134	37.0	35.0	37.0	33.0	37.0
7	36.05165653920449	37.0	35.0	37.0	35.0	37.0
8	36.08581395966177	37.0	35.0	37.0	35.0	37.0
9	37.85150010525646	39.0	38.0	39.0	35.0	39.0
10	37.37432285522205	39.0	37.0	39.0	35.0	39.0
11	37.05004589922666	39.0	37.0	39.0	33.0	39.0
12	36.62567529275805	39.0	35.0	39.0	33.0	39.0
13	36.501727008561886	39.0	35.0	39.0	32.0	39.0
14	37.59947044577502	40.0	36.0	41.0	33.0	41.0
15	37.76315505172383	40.0	36.0	41.0	33.0	41.0
16	37.834988730458875	40.0	36.0	41.0	33.0	41.0
17	37.748200608326805	40.0	35.0	41.0	33.0	41.0
18	37.63638922791142	39.0	36.0	41.0	33.0	41.0
19	37.54323324400424	39.0	36.0	41.0	33.0	41.0
20	37.38504666781492	39.0	35.0	41.0	32.0	41.0
21	37.27164748900054	39.0	35.0	41.0	32.0	41.0
22	37.18708240809506	39.0	35.0	41.0	32.0	41.0
23	37.08618312896294	39.0	35.0	41.0	32.0	41.0
24	37.118363826841076	39.0	35.0	41.0	32.0	41.0
25	37.089447622716385	39.0	35.0	41.0	32.0	41.0
26	36.991059065236165	39.0	35.0	41.0	32.0	41.0
27	36.914290370792905	39.0	35.0	41.0	32.0	41.0
28	36.86553779880026	39.0	35.0	41.0	31.0	41.0
29	36.868661539040886	39.0	35.0	41.0	31.0	41.0
30	36.781741923495524	39.0	35.0	41.0	31.0	41.0
31	36.63056524264856	38.0	35.0	41.0	31.0	41.0
32	36.476946364886196	39.0	35.0	41.0	30.0	41.0
33	36.3336123709991	38.0	35.0	41.0	30.0	41.0
34	36.18027253090249	38.0	35.0	41.0	30.0	41.0
35	36.054725336187914	38.0	35.0	41.0	30.0	41.0
36	35.94357265751892	38.0	35.0	41.0	29.0	41.0
37	35.83359230745012	38.0	35.0	41.0	29.0	41.0
38	35.71964307872381	38.0	35.0	41.0	28.0	41.0
39	35.62326396284107	38.0	35.0	40.0	27.0	41.0
40	35.49468069016139	38.0	35.0	40.0	27.0	41.0
41	35.43599203137568	38.0	35.0	40.0	27.0	41.0
42	35.35725278775296	38.0	35.0	40.0	26.0	41.0
43	35.296615186686694	38.0	34.0	40.0	26.0	41.0
44	35.12154930107855	38.0	34.0	40.0	26.0	41.0
45	34.983519492200834	38.0	34.0	40.0	25.0	41.0
46	34.953083397073684	38.0	34.0	40.0	25.0	41.0
47	34.86685767165292	38.0	34.0	40.0	24.0	41.0
48	34.76157713076434	37.0	34.0	40.0	24.0	41.0
49	34.629254012555464	37.0	34.0	40.0	24.0	41.0
50	34.54669096516783	37.0	34.0	40.0	24.0	41.0
51	34.207792929358405	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	6.0
14	11.0
15	27.0
16	98.0
17	200.0
18	553.0
19	1049.0
20	1891.0
21	3067.0
22	4913.0
23	7393.0
24	11402.0
25	17253.0
26	23259.0
27	26658.0
28	28569.0
29	31089.0
30	35860.0
31	42689.0
32	53026.0
33	68654.0
34	116069.0
35	171852.0
36	113899.0
37	150793.0
38	244440.0
39	465066.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.723002025492438	24.257694988372403	30.102052470193286	13.917250515941879
2	31.42538242667699	25.425270070802718	30.211938984586872	12.937408517933418
3	28.135762936513377	25.221424413202925	33.38068330891753	13.26212934136616
4	24.721564240705792	28.1118718797323	32.805816330247254	14.36074754931466
5	23.08987296995468	31.81461527681833	31.248329323710237	13.847182429516753
6	21.29446313955649	40.153828773351655	27.83536530783966	10.71634277925219
7	88.62551108032642	2.9634787848033124	6.887168156616681	1.5238419782535821
8	89.38335762566109	2.243413445541046	6.579671118305179	1.7935578104926804
9	84.87362742174753	5.0463838385334965	7.884172205749534	2.1958165339694404
10	42.50064666361701	36.32385160875709	12.088257391257109	9.087244336368794
11	29.565275367579652	24.411227438539175	29.627009364429984	16.396487829451193
12	29.06010607135339	23.036966934653947	30.189899947711307	17.71302704628136
13	22.53093336247178	29.835978943768353	30.225211793909697	17.407875899850172
14	17.190387028946454	32.95144682881718	31.015160017606537	18.843006124629827
15	16.252215478811966	27.364211443877934	39.33758186699657	17.04599121031353
16	20.93696156379622	25.180186103306905	36.27125424344061	17.611598089456265
17	19.90322578653742	24.873244670967058	29.58694400047412	25.636585542021407
18	22.020640144506938	25.8688905721692	32.987561216974626	19.122908066349233
19	24.61322107623346	26.653714874127466	28.978925865495203	19.75413818414387
20	25.784623666468498	27.024736195197956	28.62988184730343	18.560758291030112
21	23.345513450911902	27.095730291575844	31.416122327149438	18.142633930362816
22	23.077217500600362	23.68776672945014	30.595492307017985	22.639523462931514
23	19.192852684780657	28.061558672299274	30.755753762841444	21.989834880078625
24	19.652338823337672	26.487835624590627	34.888783117974285	18.971042434097416
25	19.533747815388185	27.808017147234963	32.0048177211142	20.653417316262647
26	18.476985257304214	31.40494847371953	29.158756998320218	20.95930927065604
27	18.515075133360867	30.027848205979186	31.961295253334715	19.495781407325232
28	17.083031608423727	28.083659443171694	35.406114011580065	19.427194936824517
29	18.044044737392838	26.241023105183004	34.781859835429515	20.93307232199465
30	19.6111622474385	27.042268650303452	32.822052371418884	20.524516730839157
31	23.27025970875135	26.683347192615624	29.776961242779436	20.269431855853586
32	24.04718205911277	26.7619345706061	29.830608086042375	19.360275284238753
33	22.368449482761708	27.377422519203904	29.87252546990375	20.38160252813064
34	19.195013374670417	27.08412430016798	31.669478650223198	22.051383674938403
35	19.814575767060344	27.13326456166084	31.06621403300176	21.985945638277055
36	23.429286484637803	26.645874656527475	30.23255813953488	19.692280719299838
37	20.162693775299363	29.33945240710114	30.410660720448092	20.08719309715141
38	20.509762305591927	30.1335985425838	28.217313546352663	21.13932560547161
39	19.980455016597183	29.001829178326677	29.270372064625615	21.74734374045052
40	22.02662834220142	26.662789771664464	29.229380690717	22.081201195417115
41	19.21464478566882	26.093602320704413	29.51656724406474	25.175185649562025
42	21.45509499936105	27.51854643600376	28.24490864294476	22.781449921690424
43	21.321193960192684	27.35198811250157	29.018929495454216	22.307888431851534
44	20.332030128659824	29.01541065763375	29.51348054422222	21.139078669484206
45	19.345644326985227	29.90258992636986	28.026123358107185	22.725642388537725
46	21.80481809151818	28.23972298720933	28.673898187057716	21.28156073421477
47	20.343265716086584	27.551080252343883	30.05266527271302	22.052988758856515
48	21.136609309610193	26.5290122004898	30.750938511087117	21.583439978812894
49	21.1288925600039	25.36396821193034	31.105538588995422	22.401600639070335
50	19.944834500414544	28.202929525086535	30.101805534205884	21.75043044029304
51	19.125809564201194	28.787735677249728	28.91521638074566	23.17123837780342
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	716.0
1	1248.5
2	1781.0
3	11169.0
4	20557.0
5	14815.5
6	9074.0
7	8357.0
8	7640.0
9	8188.5
10	8737.0
11	8927.5
12	9118.0
13	9063.5
14	9009.0
15	8679.0
16	8349.0
17	7944.0
18	7539.0
19	7772.0
20	8005.0
21	8136.5
22	8268.0
23	9515.5
24	10763.0
25	11819.5
26	15910.5
27	18945.0
28	23371.5
29	27798.0
30	30914.5
31	34031.0
32	39676.0
33	45321.0
34	48529.0
35	51737.0
36	56973.0
37	62209.0
38	69501.0
39	76793.0
40	91027.0
41	105261.0
42	115595.5
43	125930.0
44	130363.5
45	134797.0
46	136329.0
47	137861.0
48	138276.0
49	138691.0
50	131316.0
51	123941.0
52	112132.0
53	100323.0
54	91717.5
55	83112.0
56	73583.0
57	64054.0
58	57711.5
59	51369.0
60	45243.0
61	39117.0
62	33938.0
63	28759.0
64	24508.0
65	20257.0
66	15905.0
67	11553.0
68	9049.0
69	6545.0
70	5304.5
71	4064.0
72	3091.0
73	2118.0
74	1773.5
75	1108.0
76	787.0
77	568.5
78	350.0
79	256.5
80	163.0
81	109.5
82	56.0
83	40.5
84	25.0
85	17.0
86	9.0
87	9.5
88	10.0
89	6.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1619853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.041319935139207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.9998648762103	18.271370665163207
2	7.90441604549823	3.8006519010054167
3	2.7527548930989387	1.9853958326403454
4	1.4800534311548557	1.4232975223797766
5	0.9066827800837599	1.0898925397837567
6	0.7085487028024866	1.022066763222147
7	0.5481795439387765	0.922527185841158
8	0.46968440120686816	0.903346636636688
9	0.4096546592625364	0.886377485362597
>10	7.450883307274259	46.99511078253346
>50	1.3135733426516991	19.45428471091418
>100	0.053361324538262464	2.028071570399841
>500	0.0015617948527338827	0.2215934767425565
>1k	5.205982842446274E-4	0.17184289385344823
>5k	0.0	0.0
>10k+	2.602991421223137E-4	0.8241700335214541
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13170	0.8130367385188656	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8520199055099444E-4	0.0	0.0	0.06266000680308645	0.0
2	1.8520199055099444E-4	0.0	0.0	0.20909304733207273	0.0
3	1.8520199055099444E-4	0.0	0.0	0.3191647637162138	0.0
4	1.8520199055099444E-4	0.0	0.0	0.4953535907270598	0.0
5	1.8520199055099444E-4	0.0	0.0	0.8837838989093455	0.0
6	1.8520199055099444E-4	0.0	0.0	1.3527153389844635	0.0
7	1.8520199055099444E-4	0.0	0.0	1.6098991698629443	0.0
8	1.8520199055099444E-4	0.0	0.0	2.1801360987694562	0.0
9	1.8520199055099444E-4	0.0	0.0	2.431516933944006	0.0
10	1.8520199055099444E-4	0.0	0.0	2.875199169307338	0.0
11	1.8520199055099444E-4	0.0	0.0	3.429138323045363	0.0
12	1.8520199055099444E-4	0.0	0.0	3.8408423480402236	0.0
13	1.8520199055099444E-4	0.0	0.0	4.019870938906185	0.0
14	1.8520199055099444E-4	0.0	0.0	4.097594040940752	0.0
15	1.8520199055099444E-4	0.0	0.0	4.211554999126464	0.0
16	1.8520199055099444E-4	0.0	0.0	4.441143733412846	0.0
17	1.8520199055099444E-4	0.0	0.0	4.748455569733797	0.0
18	2.469359874013259E-4	0.0	0.0	5.128057916366485	0.0
19	2.469359874013259E-4	0.0	0.0	5.342460087427686	0.0
20	2.469359874013259E-4	0.0	0.0	5.568715185884151	0.0
21	2.469359874013259E-4	0.0	0.0	5.841209047981514	0.0
22	2.469359874013259E-4	0.0	0.0	6.14759487434971	0.0
23	2.469359874013259E-4	0.0	0.0	6.48478596514622	0.0
24	2.469359874013259E-4	0.0	0.0	6.733018366481403	0.0
25	2.469359874013259E-4	0.0	0.0	6.946556261586699	0.0
26	3.086699842516574E-4	0.0	0.0	7.144907593466814	0.0
27	3.086699842516574E-4	0.0	0.0	7.353445034827234	0.0
28	3.086699842516574E-4	0.0	0.0	7.571304309712054	0.0
29	3.086699842516574E-4	0.0	0.0	7.815524001251966	0.0
30	3.086699842516574E-4	0.0	0.0	8.115119087966624	0.0
31	3.086699842516574E-4	0.0	0.0	8.372241184848255	0.0
32	3.086699842516574E-4	0.0	0.0	8.61534966444486	0.0
33	3.086699842516574E-4	0.0	0.0	8.855865316173752	0.0
34	3.086699842516574E-4	0.0	0.0	9.098418189798704	0.0
35	3.086699842516574E-4	0.0	0.0	9.38461699919684	0.0
36	3.086699842516574E-4	0.0	0.0	9.636800376330445	0.0
37	3.086699842516574E-4	0.0	0.0	9.896083163101837	0.0
38	3.086699842516574E-4	0.0	0.0	10.16474951739448	0.0
39	3.086699842516574E-4	0.0	0.0	10.506261987970513	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.0341444E-4	45.0	1
ACGGCGT	20	7.0341444E-4	45.0	31
CGTTTTT	5920	0.0	42.111485	1
TAATACG	75	0.0	42.0	1
TACGGGA	725	0.0	41.27586	4
CTCCGAT	45	1.9292202E-8	40.0	30
CGTAAGG	250	0.0	39.6	2
AGGGCGA	1155	0.0	39.545456	6
GCGAACG	40	3.4593904E-7	39.375	1
ACGTGCG	40	3.4593904E-7	39.375	1
TTGCGAG	150	0.0	39.0	1
CTAGCGG	215	0.0	38.720932	2
GGGCGAT	2250	0.0	38.7	7
CGTTAGG	170	0.0	38.38235	2
GACCGAT	685	0.0	38.10219	9
TAGGGCG	480	0.0	37.968754	5
TGTAGCG	190	0.0	37.894737	1
TAGGGAT	2710	0.0	37.776752	5
TCACGAC	155	0.0	37.741936	25
ATAGGGA	2205	0.0	37.65306	4
>>END_MODULE