FastQCFastQC Report
Thu 26 May 2016
SRR1547859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547859_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2711098
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412021.5197532512657235No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC130370.4808752763640414No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG87290.32197286855731516No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC52920.19519766530018465No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.15993519968662143No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT34420.12695963037854036No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTT31810.11733253464094621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT187650.043.24941
GACCGAT11250.039.49
TACGCGG1500.039.0000042
AACGGGA10600.037.9952854
CGGTTTT17900.037.9608961
TACGGGA8950.037.9608964
AGGGATT45900.037.696086
TAATGCG900.037.5000041
TAGGGAT35250.037.4680865
ACGGGAT10900.037.3623855
GGGCGAT34100.037.280067
TAGGGAC28850.037.123055
TTAGGGA36000.036.754
CGACGGT2350.036.3829828
CGAGGGA16800.036.2946434
GAGGGAT41200.036.1529125
AGGGATG45050.036.0099876
ACTAACG504.883077E-835.9999961
ATAGGGA31950.035.9154934
AGTACGG3450.035.8695642
Produced by FastQC (version 0.11.5)