##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547859_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2711098
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40392933047791	31.0	31.0	33.0	30.0	34.0
2	31.774603500131683	31.0	31.0	34.0	30.0	34.0
3	31.620448246430044	31.0	31.0	34.0	30.0	34.0
4	35.54110437911134	37.0	35.0	37.0	33.0	37.0
5	35.54328393883216	37.0	35.0	37.0	33.0	37.0
6	35.613842066941146	37.0	35.0	37.0	33.0	37.0
7	35.90393781412549	37.0	35.0	37.0	35.0	37.0
8	35.904211135119425	37.0	35.0	37.0	35.0	37.0
9	37.64796440409015	39.0	37.0	39.0	35.0	39.0
10	37.272338735080766	39.0	37.0	39.0	34.0	39.0
11	37.0142141671013	39.0	37.0	39.0	33.0	39.0
12	36.60281295622659	39.0	35.0	39.0	32.0	39.0
13	36.48636530291417	39.0	35.0	39.0	32.0	39.0
14	37.58103358860506	40.0	36.0	41.0	32.0	41.0
15	37.6880204994434	40.0	36.0	41.0	33.0	41.0
16	37.75978662519761	40.0	36.0	41.0	33.0	41.0
17	37.64898391721731	40.0	36.0	41.0	32.0	41.0
18	37.45732872806516	39.0	36.0	41.0	32.0	41.0
19	37.320360975516195	39.0	36.0	41.0	32.0	41.0
20	37.12013250719819	39.0	35.0	41.0	32.0	41.0
21	37.05307812554175	39.0	35.0	41.0	32.0	41.0
22	36.9673412764865	39.0	35.0	41.0	32.0	41.0
23	36.890891808411205	38.0	35.0	41.0	32.0	41.0
24	36.8960240463458	39.0	35.0	41.0	32.0	41.0
25	36.87485992760129	38.0	35.0	41.0	32.0	41.0
26	36.75564439205075	38.0	35.0	41.0	31.0	41.0
27	36.68829566470854	38.0	35.0	41.0	31.0	41.0
28	36.58220396311753	38.0	35.0	41.0	31.0	41.0
29	36.541502372839346	38.0	35.0	41.0	31.0	41.0
30	36.44420415639715	38.0	35.0	40.0	31.0	41.0
31	36.2498035851157	38.0	35.0	40.0	30.0	41.0
32	36.04839293894946	38.0	35.0	40.0	30.0	41.0
33	35.8296841353577	38.0	35.0	40.0	29.0	41.0
34	35.54746563938301	38.0	35.0	40.0	27.0	41.0
35	35.3516578891652	38.0	34.0	40.0	25.0	41.0
36	35.20811457202949	38.0	34.0	40.0	25.0	41.0
37	35.107238838286186	38.0	34.0	40.0	24.0	41.0
38	34.967788696683044	38.0	34.0	40.0	24.0	41.0
39	34.84732016326964	38.0	34.0	40.0	23.0	41.0
40	34.68182153503857	38.0	34.0	40.0	23.0	41.0
41	34.58689542023195	38.0	34.0	40.0	23.0	41.0
42	34.52959096277597	38.0	34.0	40.0	23.0	41.0
43	34.47719079133252	38.0	34.0	40.0	23.0	41.0
44	34.26165634735447	37.0	33.0	40.0	22.0	41.0
45	34.15392767063382	37.0	33.0	40.0	22.0	41.0
46	34.121353414741925	37.0	33.0	40.0	22.0	41.0
47	34.03122092967499	37.0	33.0	40.0	22.0	41.0
48	33.92311417735545	37.0	33.0	40.0	21.0	41.0
49	33.76975638652679	36.0	33.0	40.0	20.0	41.0
50	33.71926024068477	36.0	33.0	40.0	20.0	41.0
51	33.39213447835527	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	4.0
11	5.0
12	11.0
13	22.0
14	31.0
15	72.0
16	252.0
17	605.0
18	1395.0
19	2570.0
20	4316.0
21	6768.0
22	10496.0
23	15634.0
24	24013.0
25	37753.0
26	51970.0
27	58571.0
28	59143.0
29	61177.0
30	66906.0
31	78528.0
32	95667.0
33	122696.0
34	196190.0
35	263701.0
36	205045.0
37	257752.0
38	396360.0
39	693357.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.040449293976092	23.79379129784316	27.318488671379644	17.847270736801104
2	34.48573234903349	26.1987209610276	26.809617357985584	12.505929331953327
3	27.821126348070045	25.8677111635212	33.29171427960184	13.01944820880691
4	24.486462680434272	26.003154441484593	34.98375934768865	14.526623530392483
5	23.258841989481752	29.48657702524955	32.20104916900828	15.053531816260424
6	20.58682496907157	38.65094511522638	30.18813779509262	10.574092120609436
7	82.6936171248697	3.0887854293721584	12.052681238376481	2.164916207381659
8	82.49679650090111	3.345139128131849	11.511756491281393	2.6463078796856476
9	77.34416092667989	4.999376636329635	13.001153038363054	4.65530939862742
10	39.12912775561783	32.12794225808141	17.974672992271028	10.768256994029725
11	29.785348961933504	21.637100540076382	31.03200253181552	17.545547966174592
12	28.458654021359614	20.01078529806005	33.3920426336488	18.138518046931537
13	23.828906221759595	23.960956040689048	34.18205465092004	18.028083086631323
14	18.324014845645564	28.278837577985012	34.11005430272163	19.2870932736478
15	17.20472664580919	25.086072137561978	40.32558026305209	17.38362095357674
16	21.629686569795705	23.951882226315686	36.21783498789051	18.200596215998093
17	21.609067617622085	23.81319303101548	32.04417545953706	22.533563891825377
18	22.068623118751148	25.023957083071142	33.85465962499327	19.052760173184442
19	23.72761884668131	26.615231172019605	30.027538657768922	19.62961132353017
20	24.37104818785599	26.7162972345522	30.58889792991622	18.32375664767559
21	23.420473918685346	26.163384724565468	32.418156776331955	17.997984580417235
22	22.149697281322915	23.02546791004973	32.73581405024827	22.08902075837908
23	20.119375987146167	25.957711598769208	32.66152680574439	21.261385608340237
24	21.05508543033118	24.909243413554215	34.308276572812936	19.727394583301674
25	21.26481595279846	27.033622539649986	31.135650574047858	20.565910933503694
26	19.5346313560041	28.237968527880586	30.201047693591303	22.02635242252401
27	19.627951479437485	26.72994484153653	32.34327936503955	21.298824313986437
28	18.2842523582696	26.32461091410196	34.31779301227768	21.073343715350752
29	19.74639057680689	25.81304696473532	33.14907834390347	21.291484114554322
30	20.876891945624983	25.7089562974116	33.0095038984205	20.404647858542923
31	23.61508141719702	24.90489093348894	30.85963694414588	20.620390705168166
32	24.928386948756557	25.830825739239234	29.804271184590153	19.43651612741406
33	22.93970929859415	26.294180439069336	29.681295180034066	21.08481508230245
34	20.371192778719177	26.81640427605347	32.28109053970015	20.531312405527206
35	21.17367206939771	26.923740860713995	31.00146140051005	20.90112566937824
36	23.595532142327574	26.916621973827574	29.569753657005393	19.918092226839455
37	21.647096490056796	27.873946275641824	29.9387923269465	20.540164907354878
38	21.413464212654798	28.513834616085436	28.653077092749875	21.419624078509887
39	21.637469394319204	27.06586040047243	29.017689511777146	22.278980693431222
40	23.194624465806843	26.097581127646436	29.422507043271768	21.285287363274954
41	19.601984140742974	25.85085452462434	30.223547802403306	24.323613532229377
42	21.345816344521666	26.50671425378205	29.193448558480732	22.954020843215552
43	21.722674724410552	25.463410027966525	29.58804882744925	23.22586642017367
44	21.422796224998137	27.123254120655172	29.383888004048544	22.070061650298143
45	20.489004823875785	27.41645635827255	28.484363162084144	23.610175655767517
46	22.218562368457356	27.36621841040051	28.764876813748526	21.650342407393612
47	20.860809900638046	26.308676410812147	31.016768851587067	21.813744836962737
48	21.407046148829735	25.56967693532288	30.5469960879319	22.476280827915478
49	21.053130502844237	24.670299635055613	31.357516401103908	22.919053460996246
50	19.88109614628464	25.873575945982036	32.07464281999396	22.170685087739358
51	19.429139042557665	26.681182310635766	30.204072298382428	23.685606348424145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1166.0
1	2460.0
2	3754.0
3	34071.0
4	64388.0
5	45732.5
6	27077.0
7	24055.0
8	21033.0
9	21819.5
10	22606.0
11	23098.0
12	23590.0
13	22722.5
14	21855.0
15	20586.5
16	19318.0
17	18018.5
18	16719.0
19	15846.0
20	14973.0
21	14952.5
22	14932.0
23	16044.0
24	17156.0
25	18710.5
26	23358.0
27	26451.0
28	30936.0
29	35421.0
30	40973.5
31	46526.0
32	53169.5
33	59813.0
34	66895.5
35	73978.0
36	79164.0
37	84350.0
38	94791.5
39	105233.0
40	118684.0
41	132135.0
42	143661.5
43	155188.0
44	168337.5
45	181487.0
46	211557.0
47	241627.0
48	231087.5
49	220548.0
50	209824.5
51	199101.0
52	183012.0
53	166923.0
54	154013.0
55	141103.0
56	133374.0
57	125645.0
58	119576.5
59	113508.0
60	104039.5
61	94571.0
62	82634.0
63	70697.0
64	62173.5
65	53650.0
66	44300.5
67	34951.0
68	29469.5
69	23988.0
70	19507.5
71	15027.0
72	12109.0
73	9191.0
74	7304.0
75	4236.0
76	3055.0
77	2205.0
78	1355.0
79	1044.5
80	734.0
81	502.5
82	271.0
83	205.5
84	140.0
85	104.5
86	69.0
87	56.0
88	43.0
89	35.5
90	28.0
91	28.0
92	28.0
93	17.5
94	7.0
95	5.5
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2711098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.085234777058602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89121306205897	18.278576841154663
2	7.333841317994255	3.53274579923169
3	2.7822291564015242	2.010319273065252
4	1.6109906253357134	1.5520434973940445
5	1.044173486752122	1.2574581788202372
6	0.7760885410121292	1.1215364830837204
7	0.6369235015025021	1.07383164480998
8	0.5221130359711764	1.0060172041222901
9	0.45907734549001006	0.9951287082260164
>10	7.882758497305939	48.1423157548394
>50	1.0088904330659418	14.881452472128126
>100	0.04561030759270434	1.8260916907509546
>500	0.0026549159433525227	0.44256960331571293
>1k	0.002811087469432083	1.3123621842329785
>5k	3.123430521591203E-4	0.5273898018094179
>10k+	3.123430521591203E-4	2.040160863015549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41202	1.5197532512657235	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	13037	0.4808752763640414	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	8729	0.32197286855731516	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	5292	0.19519766530018465	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4336	0.15993519968662143	No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	3442	0.12695963037854036	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTT	3181	0.11733253464094621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6885424281969885E-5	0.0	0.0	0.06960279562007718	0.0
2	3.6885424281969885E-5	0.0	0.0	0.26697670095289805	0.0
3	3.6885424281969885E-5	0.0	0.0	0.4372029340141891	0.0
4	3.6885424281969885E-5	0.0	0.0	0.7743357119513938	0.0
5	3.6885424281969885E-5	0.0	0.0	1.7965045896533434	0.0
6	3.6885424281969885E-5	0.0	0.0	2.7147303417287016	0.0
7	3.6885424281969885E-5	0.0	0.0	3.31238487136946	0.0
8	3.6885424281969885E-5	0.0	0.0	4.215008088973545	0.0
9	3.6885424281969885E-5	0.0	0.0	4.778506715729199	0.0
10	3.6885424281969885E-5	0.0	0.0	5.801782156159607	0.0
11	3.6885424281969885E-5	0.0	0.0	6.380145608900896	0.0
12	3.6885424281969885E-5	0.0	0.0	7.013210145852344	0.0
13	3.6885424281969885E-5	0.0	0.0	7.253076059957995	0.0
14	3.6885424281969885E-5	0.0	0.0	7.399843163175953	0.0
15	3.6885424281969885E-5	0.0	0.0	7.542368442601485	0.0
16	3.6885424281969885E-5	0.0	0.0	7.751840767098792	0.0
17	3.6885424281969885E-5	0.0	0.0	7.966034425904191	0.0
18	3.6885424281969885E-5	0.0	0.0	8.255806319063346	0.0
19	3.6885424281969885E-5	0.0	0.0	8.41765956081263	0.0
20	3.6885424281969885E-5	0.0	0.0	8.604779318195064	0.0
21	3.6885424281969885E-5	0.0	0.0	8.808866370747202	0.0
22	3.6885424281969885E-5	0.0	0.0	9.039806012176616	0.0
23	3.6885424281969885E-5	0.0	0.0	9.269823517998981	0.0
24	3.6885424281969885E-5	0.0	0.0	9.453623587196036	0.0
25	3.6885424281969885E-5	0.0	0.0	9.61492354758109	0.0
26	3.6885424281969885E-5	0.0	0.0	9.772866934356486	0.0
27	3.6885424281969885E-5	0.0	0.0	9.953347315368164	0.0
28	3.6885424281969885E-5	0.0	0.0	10.122946496216661	0.0
29	3.6885424281969885E-5	0.0	0.0	10.31895564085105	0.0
30	3.6885424281969885E-5	0.0	0.0	10.555428095922759	0.0
31	3.6885424281969885E-5	0.0	0.0	10.744465895367854	0.0
32	3.6885424281969885E-5	0.0	0.0	10.938483227091016	0.0
33	7.377084856393977E-5	0.0	0.0	11.12973415199303	0.0
34	7.377084856393977E-5	0.0	0.0	11.327144942750133	0.0
35	7.377084856393977E-5	0.0	0.0	11.548494373866234	0.0
36	7.377084856393977E-5	0.0	0.0	11.74896665483874	0.0
37	7.377084856393977E-5	0.0	0.0	11.957996354244663	0.0
38	7.377084856393977E-5	0.0	0.0	12.187571234975644	0.0
39	7.377084856393977E-5	0.0	0.0	12.506076873650455	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	18765	0.0	43.2494	1
GACCGAT	1125	0.0	39.4	9
TACGCGG	150	0.0	39.000004	2
AACGGGA	1060	0.0	37.995285	4
CGGTTTT	1790	0.0	37.960896	1
TACGGGA	895	0.0	37.960896	4
AGGGATT	4590	0.0	37.69608	6
TAATGCG	90	0.0	37.500004	1
TAGGGAT	3525	0.0	37.468086	5
ACGGGAT	1090	0.0	37.362385	5
GGGCGAT	3410	0.0	37.28006	7
TAGGGAC	2885	0.0	37.12305	5
TTAGGGA	3600	0.0	36.75	4
CGACGGT	235	0.0	36.38298	28
CGAGGGA	1680	0.0	36.294643	4
GAGGGAT	4120	0.0	36.152912	5
AGGGATG	4505	0.0	36.009987	6
ACTAACG	50	4.883077E-8	35.999996	1
ATAGGGA	3195	0.0	35.915493	4
AGTACGG	345	0.0	35.869564	2
>>END_MODULE