FastQCFastQC Report
Thu 26 May 2016
SRR1547858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547858_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1407175
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.6314068967967738No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30000.21319309965000802No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGG302.1658507E-645.0000042
CTATACG302.1658507E-645.0000041
CTGAGCG600.045.0000041
GCTAACG750.045.0000041
CTTAACG253.891254E-545.01
AACCGTC351.2121927E-745.038
TCGCACC207.033828E-445.025
TTAATCG406.8175723E-945.020
GATCGCA406.8175723E-945.09
CGGCATG207.033828E-445.044
ACTCTCG207.033828E-445.037
ATTACCG207.033828E-445.01
ATAGCCG406.8175723E-945.01
CCGTGAG207.033828E-445.01
GCACGAC850.045.023
TTCGTAG551.8189894E-1245.01
CACGACG2900.045.026
TACGGCA253.891254E-545.028
TTATCCG207.033828E-445.028
AATATCG207.033828E-445.018