##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547858_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1407175
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623711336543074	31.0	31.0	33.0	30.0	34.0
2	32.04841117842486	31.0	31.0	34.0	30.0	34.0
3	32.08701618490948	33.0	31.0	34.0	30.0	34.0
4	35.855086965018565	37.0	35.0	37.0	35.0	37.0
5	35.79123278909873	37.0	35.0	37.0	35.0	37.0
6	35.837725229626734	37.0	35.0	37.0	35.0	37.0
7	36.11104446852737	37.0	35.0	37.0	35.0	37.0
8	36.146802636488	37.0	35.0	37.0	35.0	37.0
9	37.98019649297351	39.0	39.0	39.0	35.0	39.0
10	37.48489562421163	39.0	37.0	39.0	35.0	39.0
11	37.218157656297194	39.0	37.0	39.0	34.0	39.0
12	36.34976317799847	38.0	35.0	39.0	33.0	39.0
13	36.011770391031675	38.0	35.0	39.0	32.0	39.0
14	36.979748787464246	39.0	35.0	41.0	32.0	41.0
15	37.24745394140743	39.0	35.0	41.0	33.0	41.0
16	37.40746957556807	39.0	35.0	41.0	33.0	41.0
17	37.35896103896104	39.0	35.0	41.0	33.0	41.0
18	37.288535541066324	38.0	35.0	41.0	33.0	41.0
19	37.20545276884538	38.0	35.0	41.0	33.0	41.0
20	37.03909819318848	38.0	35.0	41.0	33.0	41.0
21	36.90916836925045	38.0	35.0	41.0	33.0	41.0
22	36.80315525787482	38.0	35.0	41.0	32.0	41.0
23	36.74938937943042	38.0	35.0	41.0	32.0	41.0
24	36.76100342885569	38.0	35.0	41.0	32.0	41.0
25	36.71813882424006	38.0	35.0	41.0	32.0	41.0
26	36.6052587631247	38.0	35.0	40.0	32.0	41.0
27	36.52248938476025	38.0	35.0	40.0	32.0	41.0
28	36.52249151669124	38.0	35.0	40.0	32.0	41.0
29	36.58268303515909	38.0	35.0	40.0	32.0	41.0
30	36.527635155542136	38.0	35.0	40.0	32.0	41.0
31	36.34778261410273	37.0	35.0	40.0	31.0	41.0
32	36.13626308028497	37.0	35.0	40.0	31.0	41.0
33	36.033636896619115	37.0	35.0	40.0	31.0	41.0
34	35.91490326363103	37.0	35.0	40.0	30.0	41.0
35	35.82075221631993	37.0	35.0	40.0	30.0	41.0
36	35.689166592641286	37.0	35.0	40.0	30.0	41.0
37	35.56421909144208	37.0	35.0	40.0	30.0	41.0
38	35.49086858422016	37.0	35.0	40.0	30.0	41.0
39	35.43840034110896	37.0	35.0	40.0	29.0	41.0
40	35.23587044965978	37.0	35.0	40.0	29.0	41.0
41	35.20640005685149	36.0	34.0	40.0	29.0	41.0
42	35.142002238527546	36.0	34.0	40.0	28.0	41.0
43	35.05143070335957	36.0	34.0	40.0	28.0	41.0
44	34.87775649794801	36.0	34.0	40.0	27.0	41.0
45	34.73687068061897	36.0	34.0	40.0	27.0	41.0
46	34.710431893687705	36.0	34.0	40.0	27.0	41.0
47	34.64584149093041	35.0	34.0	40.0	27.0	41.0
48	34.58020359941017	35.0	34.0	40.0	26.0	41.0
49	34.47114466928421	36.0	34.0	40.0	26.0	41.0
50	34.3636356529927	35.0	34.0	40.0	26.0	41.0
51	34.004957450210526	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	3.0
13	4.0
14	11.0
15	15.0
16	67.0
17	170.0
18	373.0
19	748.0
20	1310.0
21	2251.0
22	3692.0
23	5815.0
24	9134.0
25	13570.0
26	17801.0
27	20249.0
28	21888.0
29	25116.0
30	30509.0
31	36875.0
32	48359.0
33	65615.0
34	133146.0
35	237194.0
36	92620.0
37	114047.0
38	181439.0
39	345084.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.142253095741466	24.694440989926626	31.24664665020342	12.916659264128485
2	29.353705118411	27.13884200614707	30.46351733082239	13.04393554461954
3	27.9290777621831	25.272904933643648	33.03142821610674	13.766589088066517
4	25.180308064028996	28.098424147671754	32.11334766464725	14.607920123651999
5	23.151420399026417	32.12450477019561	30.989535772025512	13.734539058752466
6	20.743546467212678	40.322490095403914	27.684189955051785	11.249773482331623
7	88.45211149999112	3.864622381722245	6.12965693677048	1.5536091815161583
8	89.83253682022492	2.507079787517544	5.428606960754704	2.231776431502834
9	86.8553662479791	3.9840105175262495	7.001545649972463	2.159077584522181
10	48.52843462966582	24.385879510366514	14.159503970721483	12.926181889246186
11	42.618437649901395	23.95501625597385	20.726917405439977	12.699628688684777
12	40.28127276280491	21.94197594471192	24.28894771439231	13.487803578090857
13	21.425195871160305	39.157176612716974	24.995469646632436	14.42215786949029
14	14.608488638584397	42.24648675537868	28.372377280722016	14.772647325314903
15	13.242773642226446	25.712793362588165	47.38714090287278	13.657292092312613
16	15.175724412386518	20.89114715653703	46.295734361397834	17.63739406967861
17	15.74409721605344	21.91006804413097	28.99824115692789	33.347593582887704
18	20.424964911969017	26.13910849752163	33.686002096398816	19.74992449411054
19	26.797448789240857	27.71965107396024	25.90587524650452	19.577024890294386
20	29.269067457849946	26.069039032103326	25.932169062128025	18.729724447918702
21	21.28825483681845	29.378790839803155	29.32179721783005	20.01115710554835
22	20.54499262707197	25.41659708280775	27.92673263808695	26.11167765203333
23	18.211309893936434	30.830067333487303	26.880309840638155	24.078312931938104
24	19.16883116883117	25.596176737079613	37.80460852417077	17.430383569918455
25	16.781210581484178	26.957485742711462	34.67649723737275	21.58480643843161
26	15.624922273349087	36.52303373780802	27.80457299198749	20.047470996855402
27	17.01742853589639	35.19548030628742	29.937498889619274	17.849592268196922
28	13.87858652974932	30.94810524632686	38.38364098282019	16.78966724110363
29	14.494146072805444	26.647147653987602	38.29338923730168	20.56531703590527
30	17.679464174676212	30.488674116581095	33.73979782187717	18.092063886865528
31	26.19340167356583	28.388011441363016	26.509851297812993	18.90873558725816
32	26.785865297493206	28.519977970046366	27.49224510100023	17.201911631460195
33	25.538188213974806	28.80231669834953	25.72693517153162	19.932559916144047
34	17.219961980563898	28.76827686677208	30.694121200277152	23.317639952386873
35	17.522198731501057	27.710057384475988	31.73265585303889	23.035088030984063
36	27.695275996233587	25.911347202728873	28.831097766802284	17.56227903423526
37	19.042407660738714	31.16776520333292	31.115746087018316	18.67408104891005
38	18.875619592445858	32.39696555154831	25.554106632082007	23.17330822392382
39	20.077104837706752	30.472542505374246	28.605965853571874	20.84438680334713
40	22.365661698083038	27.264341677474373	27.260362072947576	23.109634551495017
41	16.588341890667472	25.742569332172614	29.86522642883792	27.803862348321996
42	22.19418338159788	26.435020519835838	26.66868015705225	24.702115941514027
43	22.025263382308527	27.325243839607726	27.370085454900778	23.279407323182973
44	18.810240375219855	31.94862046298435	28.154067546680405	21.08707161511539
45	17.190328139712545	36.094515607511504	23.781477072858742	22.93367917991721
46	22.056176381757776	31.576882761561286	26.63599054843925	19.73095030824169
47	20.110149768152503	28.680867695915573	28.51777497468332	22.6912075612486
48	22.337591273295786	24.731039138699877	31.72405706468634	21.207312523317995
49	20.619610211949475	23.859576811697195	31.566933750244285	23.95387922610905
50	18.304119956650737	30.923659104233657	29.010108906141742	21.762112032973864
51	17.462078277399755	32.37983903920976	25.681027590740314	24.47705509265017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1106.0
1	1495.5
2	1885.0
3	7177.5
4	12470.0
5	9127.5
6	5785.0
7	5738.5
8	5692.0
9	6581.0
10	7470.0
11	7823.0
12	8176.0
13	8415.0
14	8654.0
15	8358.5
16	8063.0
17	7859.5
18	7656.0
19	7615.0
20	7574.0
21	7786.0
22	7998.0
23	8334.0
24	8670.0
25	9146.5
26	12464.5
27	15306.0
28	17406.5
29	19507.0
30	22982.0
31	26457.0
32	30421.0
33	34385.0
34	37097.5
35	39810.0
36	42160.5
37	44511.0
38	52481.0
39	60451.0
40	79091.0
41	97731.0
42	117613.0
43	137495.0
44	140842.0
45	144189.0
46	143992.0
47	143795.0
48	137452.5
49	131110.0
50	119982.0
51	108854.0
52	94626.0
53	80398.0
54	70326.5
55	60255.0
56	51664.5
57	43074.0
58	41488.0
59	39902.0
60	36047.5
61	32193.0
62	27005.5
63	21818.0
64	16826.0
65	11834.0
66	8563.0
67	5292.0
68	4353.0
69	3414.0
70	2619.5
71	1825.0
72	1524.0
73	1223.0
74	958.0
75	476.0
76	259.0
77	286.0
78	313.0
79	226.5
80	140.0
81	94.5
82	49.0
83	40.5
84	32.0
85	24.5
86	17.0
87	13.5
88	10.0
89	8.5
90	7.0
91	5.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1407175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.218107498540427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.49976177445713	16.231801689431652
2	9.643576770984524	4.09236897194354
3	3.534548334499398	2.2498927956058576
4	1.7331233744893877	1.4709439227259586
5	1.0544901982701214	1.1187143191526319
6	0.6607455391443818	0.8411861927247928
7	0.4788528284093223	0.7112245552418395
8	0.3436483782773135	0.5833254585589698
9	0.2847543501501853	0.5437753570947288
>10	3.0618306996144113	15.590932460181781
>50	1.7129337112634566	26.90867517733622
>100	0.9842850309184372	27.8754607623285
>500	0.006771826838103665	0.9278481464134741
>1k	3.3859134190518327E-4	0.21552802471857704
>5k	3.3859134190518327E-4	0.638322166541519
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8885	0.6314068967967738	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3000	0.21319309965000802	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.062110256364702326	0.0
2	0.0	0.0	0.0	0.2533444667507595	0.0
3	0.0	0.0	0.0	0.39860003197896493	0.0
4	0.0	0.0	0.0	0.7532822854300283	0.0
5	0.0	0.0	0.0	1.2875441931529483	0.0
6	0.0	0.0	0.0	1.9030326718425215	0.0
7	0.0	0.0	0.0	2.213655018032583	0.0
8	0.0	0.0	0.0	2.9554248760815107	0.0
9	0.0	0.0	0.0	3.225327340238421	0.0
10	0.0	0.0	0.0	3.795263559969442	0.0
11	0.0	0.0	0.0	4.593458525059072	0.0
12	0.0	0.0	0.0	5.156501501234743	0.0
13	0.0	0.0	0.0	5.385186632792652	0.0
14	0.0	0.0	0.0	5.471316645051255	0.0
15	0.0	0.0	0.0	5.61024748165651	0.0
16	0.0	0.0	0.0	5.972036171762574	0.0
17	0.0	0.0	0.0	6.371702169239789	0.0
18	0.0	0.0	0.0	6.912573063051859	0.0
19	0.0	0.0	0.0	7.169683941229769	0.0
20	0.0	0.0	0.0	7.441149821450779	0.0
21	0.0	0.0	0.0	7.823333984756693	0.0
22	0.0	0.0	0.0	8.188036313891308	0.0
23	0.0	0.0	0.0	8.582301419510722	0.0
24	0.0	0.0	0.0	8.860518414553983	0.0
25	0.0	0.0	0.0	9.092188249506991	0.0
26	0.0	0.0	0.0	9.297351075736849	0.0
27	0.0	0.0	0.0	9.512249720184057	0.0
28	0.0	0.0	0.0	9.725371755467515	0.0
29	0.0	0.0	0.0	9.980492831382024	0.0
30	0.0	0.0	0.0	10.309591912875087	0.0
31	0.0	0.0	0.0	10.607849059285448	0.0
32	0.0	0.0	0.0	10.870289764954608	0.0
33	0.0	0.0	0.0	11.119796755911667	0.0
34	0.0	0.0	0.0	11.395242240659478	0.0
35	0.0	0.0	0.0	11.74068612645904	0.0
36	0.0	0.0	0.0	12.030557677616502	0.0
37	0.0	0.0	0.0	12.327464601062413	0.0
38	0.0	0.0	0.0	12.602341570877822	0.0
39	0.0	0.0	0.0	12.953683799101036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGG	30	2.1658507E-6	45.000004	2
CTATACG	30	2.1658507E-6	45.000004	1
CTGAGCG	60	0.0	45.000004	1
GCTAACG	75	0.0	45.000004	1
CTTAACG	25	3.891254E-5	45.0	1
AACCGTC	35	1.2121927E-7	45.0	38
TCGCACC	20	7.033828E-4	45.0	25
TTAATCG	40	6.8175723E-9	45.0	20
GATCGCA	40	6.8175723E-9	45.0	9
CGGCATG	20	7.033828E-4	45.0	44
ACTCTCG	20	7.033828E-4	45.0	37
ATTACCG	20	7.033828E-4	45.0	1
ATAGCCG	40	6.8175723E-9	45.0	1
CCGTGAG	20	7.033828E-4	45.0	1
GCACGAC	85	0.0	45.0	23
TTCGTAG	55	1.8189894E-12	45.0	1
CACGACG	290	0.0	45.0	26
TACGGCA	25	3.891254E-5	45.0	28
TTATCCG	20	7.033828E-4	45.0	28
AATATCG	20	7.033828E-4	45.0	18
>>END_MODULE