FastQCFastQC Report
Thu 26 May 2016
SRR1547857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547857_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2170225
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101250.4665414876337707No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30480.1404462670921218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA302.1663964E-645.00000433
CGTATAT207.0346746E-445.018
CCGTAAA253.8919552E-545.019
CGTAGAC207.0346746E-445.043
CGCACCT207.0346746E-445.035
TAAATCG551.8189894E-1245.01
GTAACGT351.2125929E-745.032
GTACGAC207.0346746E-445.036
TACGGGA8850.041.4406784
TAACGCC2300.041.0869612
TAATGCG501.0822987E-940.51
AGTACGG3400.040.3676452
CGTTTTT57400.040.1785741
TACGCGG1850.040.1351362
CGTAAGG3450.039.7826082
TGTTACG1700.039.7058831
GCACGGT403.460118E-739.3753
CGGTCTA4400.039.37531
CCAATCG1900.039.07894524
TATGGGA21300.038.9788744