##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547857_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2170225
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53720651084565	31.0	31.0	33.0	30.0	34.0
2	31.947102258982362	31.0	31.0	34.0	30.0	34.0
3	32.01115644691219	33.0	31.0	34.0	30.0	34.0
4	35.776143026644704	37.0	35.0	37.0	35.0	37.0
5	35.67706712437651	37.0	35.0	37.0	33.0	37.0
6	35.73530348235782	37.0	35.0	37.0	33.0	37.0
7	36.04101464133903	37.0	35.0	37.0	35.0	37.0
8	36.06163508391987	37.0	35.0	37.0	35.0	37.0
9	37.813283876095795	39.0	38.0	39.0	35.0	39.0
10	37.378111025354514	39.0	37.0	39.0	35.0	39.0
11	37.08632468983631	39.0	37.0	39.0	33.0	39.0
12	36.41327972906035	38.0	35.0	39.0	33.0	39.0
13	36.16571737953438	38.0	35.0	39.0	32.0	39.0
14	37.191612390420346	39.0	35.0	41.0	32.0	41.0
15	37.39353661486712	39.0	35.0	41.0	33.0	41.0
16	37.516840419772144	39.0	35.0	41.0	33.0	41.0
17	37.43930928820745	39.0	35.0	41.0	33.0	41.0
18	37.3581006577659	39.0	35.0	41.0	33.0	41.0
19	37.286472600767205	39.0	35.0	41.0	32.0	41.0
20	37.14507804490318	39.0	35.0	41.0	32.0	41.0
21	37.022773675540556	39.0	35.0	41.0	32.0	41.0
22	36.918610282344	38.0	35.0	41.0	32.0	41.0
23	36.850994712529804	38.0	35.0	41.0	32.0	41.0
24	36.881180061975144	38.0	35.0	41.0	32.0	41.0
25	36.840670437397044	38.0	35.0	41.0	32.0	41.0
26	36.72287804259927	38.0	35.0	41.0	32.0	41.0
27	36.63986130470343	38.0	35.0	41.0	31.0	41.0
28	36.57429344883595	38.0	35.0	41.0	31.0	41.0
29	36.56368533216602	38.0	35.0	41.0	31.0	41.0
30	36.491992765727055	38.0	35.0	40.0	31.0	41.0
31	36.335008121277745	38.0	35.0	40.0	31.0	41.0
32	36.19553272126162	38.0	35.0	40.0	30.0	41.0
33	36.071356656567865	38.0	35.0	40.0	30.0	41.0
34	35.94596735361541	38.0	35.0	40.0	30.0	41.0
35	35.845419253764014	38.0	35.0	40.0	30.0	41.0
36	35.71437800228087	38.0	35.0	40.0	29.0	41.0
37	35.59757214113744	38.0	35.0	40.0	29.0	41.0
38	35.480965798477115	38.0	34.0	40.0	29.0	41.0
39	35.431780114965036	38.0	35.0	40.0	28.0	41.0
40	35.24485341381654	37.0	34.0	40.0	27.0	41.0
41	35.17814328007465	37.0	34.0	40.0	27.0	41.0
42	35.10030849335898	37.0	34.0	40.0	27.0	41.0
43	35.04781347556129	37.0	34.0	40.0	27.0	41.0
44	34.865609326221936	37.0	34.0	40.0	26.0	41.0
45	34.69774101763642	37.0	34.0	40.0	26.0	41.0
46	34.659507193954546	36.0	34.0	40.0	26.0	41.0
47	34.584333882431544	36.0	34.0	40.0	25.0	41.0
48	34.50359755324909	36.0	34.0	40.0	25.0	41.0
49	34.39915170086051	36.0	34.0	40.0	24.0	41.0
50	34.29907682383163	36.0	34.0	40.0	24.0	41.0
51	33.96741029155963	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	2.0
12	3.0
13	18.0
14	22.0
15	62.0
16	156.0
17	330.0
18	816.0
19	1620.0
20	2810.0
21	4775.0
22	7275.0
23	10560.0
24	15534.0
25	22338.0
26	28695.0
27	33385.0
28	36674.0
29	42161.0
30	49928.0
31	60920.0
32	76244.0
33	100728.0
34	184688.0
35	285341.0
36	150170.0
37	196055.0
38	308318.0
39	550492.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.67797117810365	23.09903351035031	29.63582117061595	12.587174140930088
2	30.862698568120816	26.221935513598822	30.02476701724476	12.890598901035608
3	29.768019444988425	25.544724625326864	31.09405706781555	13.59319886186916
4	26.02135723254501	28.00681956939949	30.471034109366542	15.50078908868896
5	23.546406478590928	32.77945835109263	28.93838196500363	14.735753205312813
6	21.672315082537523	41.215818636316506	26.5572463684641	10.554619912681865
7	88.79964059026138	3.8872927922220044	5.778479189945743	1.534587427570874
8	89.29336819914985	3.1599488532295035	5.334239537375157	2.2124434102454815
9	84.52238823163498	5.695215933831745	7.015770254236312	2.7666255802969735
10	46.78630095957793	30.698982824361533	12.121369904042208	10.39334631201834
11	38.911910055409	22.407215841675402	23.424760105519013	15.256113997396584
12	35.67418124848806	22.370537617067356	25.710099183264408	16.245181951180175
13	23.725143706297732	33.959566404405074	25.954037023810894	16.361252865486296
14	17.967722240781487	37.46606918637468	28.073632918245806	16.492575654598028
15	16.457648400511466	26.89375525579145	40.22564480641408	16.422951537283
16	19.592622884723934	23.709707518805654	38.747226670045734	17.950442926424678
17	20.061145733737284	23.581840592565285	27.739888721215543	28.617124952481888
18	22.847078067942263	26.551394440668595	30.960706839152625	19.640820652236517
19	26.868642652259556	27.394025965049707	25.76723611607091	19.97009526661982
20	28.94068587358454	26.679307445080582	25.283922173968136	19.096084507366747
21	23.5630867767167	28.33240793005334	28.744485018834453	19.360020274395513
22	23.947701275213397	24.250480940916262	27.699293852019952	24.102523931850385
23	20.79342003709293	29.620431061295488	26.760312870785285	22.825836030826295
24	20.872259788731583	26.499786888456267	33.70853252542939	18.91942079738276
25	19.93770231197226	27.5541936895944	30.97890771694179	21.52919628149155
26	19.16335863792925	32.41585549885381	26.36772684859865	22.053059014618302
27	19.470008870048037	32.11823658837217	28.860279464110867	19.55147507746893
28	17.443352647766936	29.467175062493524	33.903765738575494	19.18570655116405
29	18.420117729728485	27.195751592576805	33.40589109424138	20.97823958345333
30	20.592657443352646	29.324977824879912	30.78118628252831	19.301178449239135
31	26.638113559653952	27.768042484074236	25.681346404174683	19.912497552097133
32	26.71911898535866	28.13270513426027	26.490248706931308	18.657927173449757
33	25.358476655646307	28.835074704235737	25.355297261804647	20.45115137831331
34	20.95727401536707	28.667488394060527	28.288311119814765	22.08692647075764
35	20.869541176606113	28.298770864772088	29.288714303816427	21.54297365480538
36	27.218514209356172	27.477058830305612	26.5229642087802	18.781462751558017
37	21.33843265099241	31.086500247670173	27.593221900954973	19.98184520038245
38	20.92096441613197	31.613035514750777	25.311476920595794	22.154523148521466
39	21.075234134709532	29.895103042311284	26.824131138476424	22.20553168450276
40	23.97405798937898	26.744461979748646	26.805008697254895	22.476471333617482
41	19.499268509025562	25.968689882385465	28.62127198792752	25.91076962066145
42	22.298056653112006	27.830155859415502	26.156550588072662	23.715236899399834
43	22.57512469905194	28.331255975762883	26.43237452337891	22.661244801806266
44	21.204529484270065	29.894089322535685	26.898685620154595	22.002695573039663
45	20.18104113628771	32.92059579075902	24.707806794226407	22.190556278726863
46	23.11571380847608	30.06185994539737	26.695619117833402	20.12680712829315
47	21.83861120390743	28.993767927288644	27.725834878872007	21.44178598993192
48	22.819246852284902	26.751696252692692	29.315808268727896	21.11324862629451
49	22.403621744289186	25.33446992823325	29.37455793754104	22.88735038993653
50	20.32535796979576	30.11231554331924	27.872271308274488	21.690055178610514
51	19.906369155271918	30.761050121531174	25.77539195244733	23.557188770749576
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1134.0
1	1852.0
2	2570.0
3	9390.5
4	16211.0
5	12123.5
6	8036.0
7	7719.0
8	7402.0
9	8012.0
10	8622.0
11	9074.0
12	9526.0
13	9486.5
14	9447.0
15	9466.0
16	9485.0
17	9182.5
18	8880.0
19	8726.0
20	8572.0
21	8781.0
22	8990.0
23	10657.5
24	12325.0
25	15632.5
26	19588.0
27	20236.0
28	25156.5
29	30077.0
30	36220.5
31	42364.0
32	46755.5
33	51147.0
34	57445.5
35	63744.0
36	69434.0
37	75124.0
38	83016.5
39	90909.0
40	113013.5
41	135118.0
42	156106.5
43	177095.0
44	181421.0
45	185747.0
46	189992.0
47	194237.0
48	186555.0
49	178873.0
50	170792.0
51	162711.0
52	147912.0
53	133113.0
54	119161.5
55	105210.0
56	98035.5
57	90861.0
58	85173.0
59	79485.0
60	73600.0
61	67715.0
62	60480.5
63	53246.0
64	44464.0
65	35682.0
66	29951.5
67	24221.0
68	20098.5
69	15976.0
70	13341.0
71	10706.0
72	9245.0
73	7784.0
74	5975.0
75	3227.0
76	2288.0
77	1639.5
78	991.0
79	834.5
80	678.0
81	492.0
82	306.0
83	203.0
84	100.0
85	85.5
86	71.0
87	60.5
88	50.0
89	40.0
90	30.0
91	21.0
92	12.0
93	9.5
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2170225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.489423929081156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34310778083139	15.268620394623191
2	8.498127256939085	3.312472094275308
3	2.791561634847854	1.6321778437712602
4	1.4323215167693761	1.1166048497225156
5	0.8660117776688052	0.8439035331282261
6	0.5524412719108243	0.6460057286514509
7	0.3958709607322274	0.5400707882446113
8	0.3388895659137847	0.5283809932188833
9	0.2742744636054968	0.48109061647159806
>10	3.22923356920705	15.644265188543253
>50	2.2032746413023157	31.93500856496676
>100	1.0676972931219664	26.49970512648574
>500	0.005750613719836935	0.7125708133632096
>1k	0.001198044524966028	0.3663022124661522
>5k	0.0	0.0
>10k+	2.3960890499320563E-4	0.4728212520677958
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10125	0.4665414876337707	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3048	0.1404462670921218	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.6078171618150194E-5	0.0	0.0	0.04695365687889504	0.0
2	4.6078171618150194E-5	0.0	0.0	0.15892361391100002	0.0
3	4.6078171618150194E-5	0.0	0.0	0.2308977179785506	0.0
4	4.6078171618150194E-5	0.0	0.0	0.4066398645301754	0.0
5	4.6078171618150194E-5	0.0	0.0	0.7225978873158313	0.0
6	4.6078171618150194E-5	0.0	0.0	1.102650646822334	0.0
7	4.6078171618150194E-5	0.0	0.0	1.316130815929224	0.0
8	4.6078171618150194E-5	0.0	0.0	1.8524807335644922	0.0
9	4.6078171618150194E-5	0.0	0.0	2.0568835028626062	0.0
10	4.6078171618150194E-5	0.0	0.0	2.440530359755325	0.0
11	4.6078171618150194E-5	0.0	0.0	2.9093757559700033	0.0
12	4.6078171618150194E-5	0.0	0.0	3.295649068644956	0.0
13	4.6078171618150194E-5	0.0	0.0	3.443882546740545	0.0
14	4.6078171618150194E-5	0.0	0.0	3.5123547097651167	0.0
15	4.6078171618150194E-5	0.0	0.0	3.6124364985197386	0.0
16	4.6078171618150194E-5	0.0	0.0	3.839279337395892	0.0
17	4.6078171618150194E-5	0.0	0.0	4.093538688384845	0.0
18	4.6078171618150194E-5	0.0	0.0	4.391618380582658	0.0
19	4.6078171618150194E-5	0.0	0.0	4.564641915008813	0.0
20	4.6078171618150194E-5	0.0	0.0	4.747065396445069	0.0
21	4.6078171618150194E-5	0.0	0.0	4.9850591528528145	0.0
22	4.6078171618150194E-5	0.0	0.0	5.236922438917624	0.0
23	4.6078171618150194E-5	0.0	0.0	5.500074877028879	0.0
24	4.6078171618150194E-5	0.0	0.0	5.713462889792533	0.0
25	4.6078171618150194E-5	0.0	0.0	5.888375629255031	0.0
26	4.6078171618150194E-5	0.0	0.0	6.057574675436879	0.0
27	4.6078171618150194E-5	0.0	0.0	6.2330403529587945	0.0
28	4.6078171618150194E-5	0.0	0.0	6.413436394843853	0.0
29	4.6078171618150194E-5	0.0	0.0	6.612309783547789	0.0
30	4.6078171618150194E-5	0.0	0.0	6.869702450206776	0.0
31	4.6078171618150194E-5	0.0	0.0	7.092766879010241	0.0
32	4.6078171618150194E-5	0.0	0.0	7.299473556889263	0.0
33	4.6078171618150194E-5	0.0	0.0	7.51051158290039	0.0
34	4.6078171618150194E-5	0.0	0.0	7.741271066364087	0.0
35	4.6078171618150194E-5	0.0	0.0	8.01045974495732	0.0
36	4.6078171618150194E-5	0.0	0.0	8.237671209206418	0.0
37	4.6078171618150194E-5	0.0	0.0	8.471379695653676	0.0
38	4.6078171618150194E-5	0.0	0.0	8.722413574629359	0.0
39	4.6078171618150194E-5	0.0	0.0	9.059982259903927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTAA	30	2.1663964E-6	45.000004	33
CGTATAT	20	7.0346746E-4	45.0	18
CCGTAAA	25	3.8919552E-5	45.0	19
CGTAGAC	20	7.0346746E-4	45.0	43
CGCACCT	20	7.0346746E-4	45.0	35
TAAATCG	55	1.8189894E-12	45.0	1
GTAACGT	35	1.2125929E-7	45.0	32
GTACGAC	20	7.0346746E-4	45.0	36
TACGGGA	885	0.0	41.440678	4
TAACGCC	230	0.0	41.08696	12
TAATGCG	50	1.0822987E-9	40.5	1
AGTACGG	340	0.0	40.367645	2
CGTTTTT	5740	0.0	40.178574	1
TACGCGG	185	0.0	40.135136	2
CGTAAGG	345	0.0	39.782608	2
TGTTACG	170	0.0	39.705883	1
GCACGGT	40	3.460118E-7	39.375	3
CGGTCTA	440	0.0	39.375	31
CCAATCG	190	0.0	39.078945	24
TATGGGA	2130	0.0	38.978874	4
>>END_MODULE