FastQCFastQC Report
Thu 26 May 2016
SRR1547856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547856_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2491622
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104720.4202884707230872No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC39940.16029718793621184No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT253.8921222E-545.012
GTATCGA253.8921222E-545.09
CGTTTTT55200.040.5978281
CGCATCG1400.040.1785721
GGCGATT13050.040.0000048
TACGGGT1550.039.1935464
ATAGCGG4250.039.176472
ACGGGTA2300.039.1304365
GGGCGAT35050.038.388027
TAGACGG3350.038.2835852
CGTTAGG4350.038.275862
TCACGAC2700.037.50000425
GTCGTAC301.1403044E-437.50000426
TAAGGGA28000.037.2857134
TAGGGAC23400.037.2115365
TAGGGAT34250.037.182485
ATTGGGA23150.037.127434
TTAGGGA26400.036.7329524
GACCGAT11050.036.651589
ACGGGAT12200.036.5163965
Produced by FastQC (version 0.11.5)