##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547856_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2491622
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.52204427477362	31.0	31.0	33.0	30.0	34.0
2	31.94906330093409	31.0	31.0	34.0	30.0	34.0
3	32.03810770654618	33.0	31.0	34.0	30.0	34.0
4	35.795733863322766	37.0	35.0	37.0	35.0	37.0
5	35.71600748428132	37.0	35.0	37.0	33.0	37.0
6	35.75239823697174	37.0	35.0	37.0	33.0	37.0
7	36.03134664888976	37.0	35.0	37.0	35.0	37.0
8	36.04663829425169	37.0	35.0	37.0	35.0	37.0
9	37.79032252885871	39.0	38.0	39.0	35.0	39.0
10	37.36594435271482	39.0	37.0	39.0	35.0	39.0
11	37.06967549652395	39.0	37.0	39.0	33.0	39.0
12	36.415952740825055	38.0	35.0	39.0	33.0	39.0
13	36.17551819658038	38.0	35.0	39.0	32.0	39.0
14	37.20272256385599	39.0	35.0	41.0	32.0	41.0
15	37.4178157842562	39.0	35.0	41.0	33.0	41.0
16	37.53423191800361	39.0	35.0	41.0	33.0	41.0
17	37.458216374714944	39.0	35.0	41.0	33.0	41.0
18	37.36630957665328	39.0	35.0	41.0	33.0	41.0
19	37.299734068811404	39.0	35.0	41.0	32.0	41.0
20	37.15472090068236	39.0	35.0	41.0	32.0	41.0
21	37.019185895773916	39.0	35.0	41.0	32.0	41.0
22	36.934229991547674	39.0	35.0	41.0	32.0	41.0
23	36.86118680923511	38.0	35.0	41.0	32.0	41.0
24	36.881593997805446	38.0	35.0	41.0	32.0	41.0
25	36.83706758087703	38.0	35.0	41.0	32.0	41.0
26	36.73170769884035	38.0	35.0	41.0	31.0	41.0
27	36.64863049049976	38.0	35.0	41.0	31.0	41.0
28	36.601482488114165	38.0	35.0	41.0	31.0	41.0
29	36.60083632268458	38.0	35.0	41.0	31.0	41.0
30	36.52635311455751	38.0	35.0	41.0	31.0	41.0
31	36.361988696519774	38.0	35.0	40.0	31.0	41.0
32	36.22179247092858	38.0	35.0	41.0	30.0	41.0
33	36.129576235881686	38.0	35.0	41.0	30.0	41.0
34	35.98930295205292	38.0	35.0	40.0	30.0	41.0
35	35.9107818120084	38.0	35.0	40.0	30.0	41.0
36	35.78154310726105	38.0	35.0	40.0	29.0	41.0
37	35.688030929250104	38.0	35.0	40.0	29.0	41.0
38	35.56769927380638	38.0	35.0	40.0	29.0	41.0
39	35.49872572966525	38.0	35.0	40.0	28.0	41.0
40	35.34578760341657	38.0	34.0	40.0	27.0	41.0
41	35.28723738994117	38.0	34.0	40.0	27.0	41.0
42	35.2162045446701	37.0	34.0	40.0	27.0	41.0
43	35.15049473796587	37.0	34.0	40.0	27.0	41.0
44	34.99276334853361	37.0	34.0	40.0	27.0	41.0
45	34.8492339528227	37.0	34.0	40.0	26.0	41.0
46	34.80893610668071	37.0	34.0	40.0	26.0	41.0
47	34.74206039278831	37.0	34.0	40.0	26.0	41.0
48	34.65744482911132	36.0	34.0	40.0	26.0	41.0
49	34.535704051417106	36.0	34.0	40.0	25.0	41.0
50	34.44808722992492	36.0	34.0	40.0	24.0	41.0
51	34.11213900021753	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	14.0
13	9.0
14	51.0
15	83.0
16	219.0
17	462.0
18	1081.0
19	2048.0
20	3560.0
21	5635.0
22	8384.0
23	12289.0
24	17790.0
25	24751.0
26	32354.0
27	37860.0
28	42248.0
29	48088.0
30	56779.0
31	68482.0
32	85676.0
33	114454.0
34	207474.0
35	316070.0
36	169642.0
37	225694.0
38	356821.0
39	653505.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.787468564653864	24.105181283517325	29.320940335251493	12.786409816577313
2	31.216853920859585	25.514263399504415	29.693870097470644	13.575012582165352
3	30.245398379047867	25.50242372237844	30.75767512086504	13.494502777708659
4	26.684184037546625	27.562447273302293	30.252221243832334	15.501147445318752
5	23.893511937203957	32.41242050359164	28.716113439357976	14.977954119846428
6	22.242860273348043	41.11775381659016	26.051303127039333	10.588082783022465
7	88.71353680453937	3.9598703174076966	5.688864522788769	1.6377283552641613
8	89.19595347929983	3.130691573601453	5.41871921182266	2.254635735276057
9	84.23970409636775	5.672770588797177	7.130134506759051	2.9573908080760245
10	46.936935056762216	30.576186917598253	12.358897136082438	10.127980889557083
11	38.058702323225596	22.11334624593939	23.89523772064944	15.932713710185572
12	34.79311869938538	22.32100214237954	26.153124350322805	16.732754807912276
13	24.105783300998308	32.364861122594036	26.785443377847844	16.743912198559812
14	17.885056401011067	35.68635210316814	28.88981554987073	17.538775945950068
15	17.036813770307052	25.98889398151084	40.40042992075042	16.57386232743169
16	20.640610814963104	23.217927920045657	37.8265242480601	18.31493701693114
17	20.948803630727294	23.224309305344068	27.845676430855082	27.981210633073555
18	23.4506678781934	25.0859480290349	31.79157994270399	19.671804150067707
19	27.56260781129722	26.588061913083123	25.556123681682053	20.293206593937605
20	28.70884909508746	25.97681349739246	25.921748965131954	19.39258844238813
21	24.428544939802265	26.484876116842766	29.469598518555383	19.61698042479959
22	24.415742034706707	24.101127699145376	27.94882209259671	23.534308173551207
23	21.14758980294764	28.411211652489825	27.027253732709056	23.413944811853483
24	20.998369736661502	26.150676145900142	33.66826910341938	19.18268501401898
25	20.17244188725256	26.655247063960747	31.405807140890552	21.766503907896144
26	19.273067905163785	32.027008912266794	26.903197997127975	21.796725185441453
27	19.572471265705634	30.954534837146248	29.65610353416369	19.81689036298443
28	17.831998593687164	28.110082508502494	34.28385204497311	19.774066852837226
29	18.825648513297764	25.53067840948587	34.153414924093624	21.490258153122745
30	20.81459386696698	27.827455368430687	31.0828046950942	20.275146069508136
31	26.693495241252485	26.148749689960997	26.271721794076306	20.886033274710208
32	26.825898952569855	27.42566890162312	26.501331261322946	19.247100884484084
33	25.525822135139283	27.239244155012276	26.698030439609216	20.536903270239225
34	20.360632551807615	27.879509813286283	29.225540631765174	22.534317003140924
35	20.98536615907228	27.589337387452833	29.361797254960827	22.063499198514062
36	26.75237255089255	25.6937448778346	27.85137552967505	19.7025070415978
37	21.79652451294779	29.58614910287355	28.696246862485562	19.9210795216931
38	21.360984932706486	30.335500328701542	25.92026398867886	22.38325074991311
39	21.11981672982499	28.867179692585793	28.16482596477315	21.84817761281607
40	23.221660428427747	26.806915334669547	26.791463552657667	23.179960684245042
41	19.49364711019569	25.42624844378481	28.951221332930917	26.12888311308858
42	22.813051096835714	26.541385491057635	26.25534691859359	24.390216493513062
43	22.258713400347244	26.566389283767762	27.757099592153224	23.41779772373177
44	20.798941412461442	28.909682126743142	28.05770698765704	22.23366947313838
45	19.9062297571622	31.65957757637394	25.504350178317576	22.929842488146278
46	22.69208571765701	29.26836414191238	26.890836571518474	21.14871356891214
47	21.714369194043076	27.23073564128106	28.164023274798506	22.890871889877356
48	22.334688006447205	25.73448139404773	29.786420251546986	22.144410347958075
49	22.142885237006254	24.65807413805144	30.256997249181456	22.94204337576085
50	20.442868139709798	28.920558575899552	28.78390060771658	21.85267267667407
51	19.66008487643792	30.69679108628837	26.370934274942186	23.272189762331525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1079.0
1	2129.0
2	3179.0
3	10431.5
4	17684.0
5	13164.0
6	8644.0
7	8546.0
8	8448.0
9	9031.5
10	9615.0
11	9815.0
12	10015.0
13	10171.0
14	10327.0
15	9998.5
16	9670.0
17	9320.0
18	8970.0
19	8991.5
20	9013.0
21	10338.5
22	11664.0
23	12197.5
24	12731.0
25	15622.0
26	23275.5
27	28038.0
28	32669.5
29	37301.0
30	41589.0
31	45877.0
32	51829.0
33	57781.0
34	64129.5
35	70478.0
36	76672.0
37	82866.0
38	96110.5
39	109355.0
40	128750.5
41	148146.0
42	174402.5
43	200659.0
44	203072.5
45	205486.0
46	204926.5
47	204367.0
48	202556.5
49	200746.0
50	189287.5
51	177829.0
52	164723.0
53	151617.0
54	139281.0
55	126945.0
56	121443.0
57	115941.0
58	109412.5
59	102884.0
60	93390.0
61	83896.0
62	75243.0
63	66590.0
64	57132.5
65	47675.0
66	40047.0
67	32419.0
68	27485.0
69	22551.0
70	17999.5
71	13448.0
72	11036.5
73	8625.0
74	6972.0
75	3849.0
76	2379.0
77	1892.5
78	1406.0
79	1043.5
80	681.0
81	537.0
82	393.0
83	296.0
84	199.0
85	131.5
86	64.0
87	52.5
88	41.0
89	36.0
90	31.0
91	24.5
92	18.0
93	13.0
94	8.0
95	7.5
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2491622.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.697088280689197
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.91933888217099	13.966425072514077
2	7.9170901973837635	2.802188882985591
3	2.6251729115696376	1.3937375030436534
4	1.24935817078747	0.8844000737050491
5	0.7741769273203701	0.6850338713830648
6	0.5407492885077925	0.5741812737865366
7	0.38063632325368235	0.4715308230820156
8	0.3130068379644282	0.44314477151326914
9	0.2558099683091186	0.40743824330221007
>10	3.3611868228452915	15.09479808864998
>50	2.2823704785018	30.076226820044127
>100	1.374414482087078	31.851999463232993
>500	0.005766128705729331	0.6691208758263084
>1k	6.919354446875196E-4	0.2523336335239577
>5k	0.0	0.0
>10k+	2.3064514822917318E-4	0.42744060340713125
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10472	0.4202884707230872	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3994	0.16029718793621184	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0134498732151184E-5	0.0	0.0	0.03575983837034671	0.0
2	4.0134498732151184E-5	0.0	0.0	0.13011604488963413	0.0
3	4.0134498732151184E-5	0.0	0.0	0.19718079227105878	0.0
4	4.0134498732151184E-5	0.0	0.0	0.3499728289443583	0.0
5	4.0134498732151184E-5	0.0	0.0	0.6112082811919304	0.0
6	4.0134498732151184E-5	0.0	0.0	0.9457293281244106	0.0
7	4.0134498732151184E-5	0.0	0.0	1.1128895153438203	0.0
8	4.0134498732151184E-5	0.0	0.0	1.5302080331607282	0.0
9	4.0134498732151184E-5	0.0	0.0	1.692351408038619	0.0
10	4.0134498732151184E-5	0.0	0.0	1.9874603772161268	0.0
11	4.0134498732151184E-5	0.0	0.0	2.3519618947015237	0.0
12	4.0134498732151184E-5	0.0	0.0	2.6286089944622417	0.0
13	4.0134498732151184E-5	0.0	0.0	2.74443715780323	0.0
14	4.0134498732151184E-5	0.0	0.0	2.794605281218419	0.0
15	4.0134498732151184E-5	0.0	0.0	2.8697370628450063	0.0
16	4.0134498732151184E-5	0.0	0.0	3.0463288572664715	0.0
17	4.0134498732151184E-5	0.0	0.0	3.2478441754006027	0.0
18	4.0134498732151184E-5	0.0	0.0	3.5059892712457987	0.0
19	4.0134498732151184E-5	0.0	0.0	3.6444131573729885	0.0
20	4.0134498732151184E-5	0.0	0.0	3.7869708968695894	0.0
21	4.0134498732151184E-5	0.0	0.0	3.969502597103413	0.0
22	4.0134498732151184E-5	0.0	0.0	4.157412320167345	0.0
23	4.0134498732151184E-5	0.0	0.0	4.360653421746958	0.0
24	4.0134498732151184E-5	0.0	0.0	4.513605996415187	0.0
25	4.0134498732151184E-5	0.0	0.0	4.642879216831446	0.0
26	4.0134498732151184E-5	0.0	0.0	4.777088980591759	0.0
27	4.0134498732151184E-5	0.0	0.0	4.91218170332418	0.0
28	4.0134498732151184E-5	0.0	0.0	5.048518595517297	0.0
29	4.0134498732151184E-5	0.0	0.0	5.207170269005491	0.0
30	4.0134498732151184E-5	0.0	0.0	5.396645237519977	0.0
31	4.0134498732151184E-5	0.0	0.0	5.567096453635423	0.0
32	4.0134498732151184E-5	0.0	0.0	5.720891852777027	0.0
33	4.0134498732151184E-5	0.0	0.0	5.8858847770649	0.0
34	4.0134498732151184E-5	0.0	0.0	6.066449886860848	0.0
35	4.0134498732151184E-5	0.0	0.0	6.27823963667041	0.0
36	4.0134498732151184E-5	0.0	0.0	6.454670893096947	0.0
37	4.0134498732151184E-5	0.0	0.0	6.635075464897966	0.0
38	4.0134498732151184E-5	0.0	0.0	6.831614105189311	0.0
39	4.0134498732151184E-5	0.0	0.0	7.1204219580658705	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACGT	25	3.8921222E-5	45.0	12
GTATCGA	25	3.8921222E-5	45.0	9
CGTTTTT	5520	0.0	40.597828	1
CGCATCG	140	0.0	40.17857	21
GGCGATT	1305	0.0	40.000004	8
TACGGGT	155	0.0	39.193546	4
ATAGCGG	425	0.0	39.17647	2
ACGGGTA	230	0.0	39.130436	5
GGGCGAT	3505	0.0	38.38802	7
TAGACGG	335	0.0	38.283585	2
CGTTAGG	435	0.0	38.27586	2
TCACGAC	270	0.0	37.500004	25
GTCGTAC	30	1.1403044E-4	37.500004	26
TAAGGGA	2800	0.0	37.285713	4
TAGGGAC	2340	0.0	37.211536	5
TAGGGAT	3425	0.0	37.18248	5
ATTGGGA	2315	0.0	37.12743	4
TTAGGGA	2640	0.0	36.732952	4
GACCGAT	1105	0.0	36.65158	9
ACGGGAT	1220	0.0	36.516396	5
>>END_MODULE