FastQCFastQC Report
Thu 26 May 2016
SRR1547855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547855_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3674748
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302610.8234850389741011No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT3600.043.7528
CGTTTTT139300.042.6579321
CGGTCTA3750.042.031
CGCGATT403.460991E-739.37522
CGTTCGA800.039.37514
CTCACGA4200.039.10714324
TACGGGA12500.038.884
TTAGGGA46400.038.5991364
ACGGGAC10950.038.219185
ATAGGGA49850.037.9137424
TAGGGAC40550.037.842175
TCGTTAG1250.037.81
ACGGGAT16900.037.5443765
TAAGGGA45250.037.441994
CGGTTTT14250.037.4210551
AAGGGAT58900.037.130735
TAGGGAT52500.037.0714265
GGGCGAT47600.037.0588237
GGCGATA10200.037.0588238
GTAGGGA51200.036.826174
Produced by FastQC (version 0.11.5)