##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547855_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3674748
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.435743621059185	31.0	31.0	33.0	30.0	34.0
2	31.838904327589265	31.0	31.0	34.0	30.0	34.0
3	31.84303359033055	33.0	31.0	34.0	30.0	34.0
4	35.66039059004862	37.0	35.0	37.0	33.0	37.0
5	35.59517115187218	37.0	35.0	37.0	33.0	37.0
6	35.64744113065712	37.0	35.0	37.0	33.0	37.0
7	35.997089596347834	37.0	35.0	37.0	35.0	37.0
8	36.020977765005924	37.0	35.0	37.0	35.0	37.0
9	37.74865351311165	39.0	38.0	39.0	35.0	39.0
10	37.29015540657482	39.0	37.0	39.0	34.0	39.0
11	36.99209006984969	39.0	37.0	39.0	33.0	39.0
12	36.49946744647524	39.0	35.0	39.0	32.0	39.0
13	36.33103385592699	39.0	35.0	39.0	32.0	39.0
14	37.307481220480966	40.0	35.0	41.0	32.0	41.0
15	37.50716947121272	40.0	35.0	41.0	32.0	41.0
16	37.59644389220703	40.0	35.0	41.0	33.0	41.0
17	37.51766243562824	39.0	35.0	41.0	33.0	41.0
18	37.414471414094244	39.0	35.0	41.0	32.0	41.0
19	37.34323333191827	39.0	35.0	41.0	32.0	41.0
20	37.16930548707013	39.0	35.0	41.0	32.0	41.0
21	37.07593636352751	39.0	35.0	41.0	32.0	41.0
22	36.99475120470846	39.0	35.0	41.0	32.0	41.0
23	36.89053643950551	38.0	35.0	41.0	32.0	41.0
24	36.91730371715285	39.0	35.0	41.0	32.0	41.0
25	36.831245571124875	38.0	35.0	41.0	31.0	41.0
26	36.66909213910723	38.0	35.0	41.0	31.0	41.0
27	36.5294572580215	38.0	35.0	41.0	31.0	41.0
28	36.412677005334785	38.0	35.0	40.0	31.0	41.0
29	36.31309004045992	38.0	35.0	40.0	30.0	41.0
30	36.21452001606641	38.0	35.0	40.0	30.0	41.0
31	35.9675637621954	38.0	35.0	40.0	30.0	41.0
32	35.89115743446897	38.0	35.0	40.0	30.0	41.0
33	35.76435336518313	38.0	35.0	40.0	29.0	41.0
34	35.649848370554935	38.0	35.0	40.0	28.0	41.0
35	35.586982835285575	38.0	35.0	40.0	28.0	41.0
36	35.53044725788	38.0	35.0	40.0	27.0	41.0
37	35.465072979153945	38.0	34.0	40.0	27.0	41.0
38	35.379104363074696	38.0	34.0	40.0	27.0	41.0
39	35.30334025625703	38.0	34.0	40.0	26.0	41.0
40	35.144393030488075	38.0	34.0	40.0	25.0	41.0
41	35.046971656287724	38.0	34.0	40.0	25.0	41.0
42	34.958342993859716	38.0	34.0	40.0	25.0	41.0
43	34.88461984331987	38.0	34.0	40.0	24.0	41.0
44	34.69592908139551	37.0	34.0	40.0	24.0	41.0
45	34.577540555161875	37.0	34.0	40.0	23.0	41.0
46	34.552731779158734	37.0	34.0	40.0	23.0	41.0
47	34.46841307213447	37.0	33.0	40.0	23.0	41.0
48	34.36452105015092	37.0	33.0	40.0	23.0	41.0
49	34.23452764652161	36.0	33.0	40.0	23.0	41.0
50	34.13395285880828	36.0	33.0	40.0	23.0	41.0
51	33.77185483195038	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	0.0
10	2.0
11	8.0
12	7.0
13	10.0
14	30.0
15	98.0
16	254.0
17	636.0
18	1353.0
19	2699.0
20	4855.0
21	8023.0
22	12491.0
23	18903.0
24	28672.0
25	42463.0
26	56277.0
27	65313.0
28	71471.0
29	79467.0
30	92328.0
31	110467.0
32	136063.0
33	174506.0
34	287609.0
35	401050.0
36	265861.0
37	344000.0
38	549629.0
39	920090.0
40	111.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.583907250238656	24.295761233151225	29.49200870372608	14.62832281288404
2	30.738869712970796	26.169712861943182	29.90482612685278	13.186591298233239
3	28.392341461237613	24.88453630017623	33.009773731423216	13.713348507162939
4	25.564555719194892	27.517696451566202	32.17015153147916	14.747596297759738
5	22.85032878444998	32.312011599162716	30.3947100590299	14.442949557357402
6	21.571111815014255	39.83026863338656	27.5546513665699	11.043968185029286
7	87.11808265491948	3.055801377400573	7.745646776323166	2.080469191356795
8	87.21128632493983	2.3974977331778944	7.469219658055463	2.921996283826809
9	81.89979285654417	4.791267319554974	9.881004085178086	3.427935738722764
10	42.85733334639546	32.912270446844246	13.967760510380575	10.262635696379725
11	32.10729007812236	22.699352445392172	28.637609980330623	16.555747496154837
12	31.719045768580596	21.006392819317135	28.910825994054555	18.363735418047714
13	24.334022360172725	27.423866888287307	30.472647376092183	17.769463375447785
14	17.559163240581395	31.29240426826547	31.95400065528303	19.194431835870105
15	16.989437098816026	25.397415006416768	40.63598374636846	16.977164148398746
16	21.62847629279613	23.46882017488002	37.08733224700034	17.81537128532351
17	21.173696808597487	23.995169192554155	29.208587908613055	25.622546090235303
18	22.524047907502773	24.566392035589924	32.85672922333722	20.052830833570084
19	24.834682541496722	27.10898815374551	27.83020767682573	20.226121627932038
20	25.254738556222083	27.472795413454204	27.443106302799542	19.829359727524174
21	22.845865893389153	27.219471920251404	30.6927441010921	19.241918085267343
22	21.829197539531965	23.528116757938232	30.504703996029114	24.137981706500693
23	19.28194804106295	27.985157077437687	29.780763197911803	22.952131683587552
24	20.553790355148163	25.74418708439327	33.621734061764236	20.080288498694333
25	21.128768557735114	26.412287318749478	30.547428014111443	21.911516109403966
26	19.120168240107894	29.808819543544214	28.798301271270848	22.27271094507705
27	19.42631168178063	28.189660896475075	31.25822505379961	21.125802367944686
28	18.02142623113204	27.265461468378238	33.77823458914734	20.934877711342384
29	18.455279110295457	25.85668459442661	33.16624704605594	22.52178924922199
30	21.12016932861791	25.652044711637366	32.54761959187405	20.68016636787067
31	23.99617606431788	25.044302357603843	28.59167485770453	22.367846720373752
32	24.5953191892342	25.290795450463545	29.410288814362236	20.70359654594002
33	23.284753131371186	24.729450835812415	28.653257311793894	23.3325387210225
34	18.71004487926791	25.36690951325098	32.91648842315173	23.006557184329374
35	18.688193040720073	25.26739248514456	31.673301135207094	24.371113338928275
36	23.132824346050395	24.38169909882256	30.497710319183792	21.987766235943255
37	19.439999695217196	27.527220914196022	29.942978402872793	23.08980098771399
38	20.02071978813241	26.913165202076446	29.739454242848762	23.326660766942386
39	19.22085541648026	25.996748620585684	29.34446117121501	25.43793479171905
40	22.26492809847097	24.207238156194656	30.010874215048215	23.516959530286158
41	18.2488703987321	23.92974974066249	30.230970940048135	27.590408920557273
42	21.016162196700293	25.49823824654099	29.031051925193236	24.454547631565486
43	20.97909843069511	25.210980453625666	29.222384773051104	24.58753634262812
44	20.821237265793464	27.53737127008437	28.775034369703718	22.866357094418447
45	19.549721504712707	27.583959498719366	27.62268324249717	25.243635754070752
46	21.462995557790627	26.734758410644755	28.636657534067645	23.165588497496973
47	20.45108943524835	25.482509276826605	30.0665515023071	23.99984978561795
48	20.93335379732161	24.24261473167684	30.906037638499296	23.91799383250226
49	21.608121155518692	22.960649274453647	30.726515124302402	24.704714445725255
50	19.052354066183586	26.123859377568202	30.79174408694147	24.032042469306738
51	18.427821445171205	26.924295216978145	28.894062939826075	25.753820398024573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1600.0
1	2866.0
2	4132.0
3	25356.0
4	46580.0
5	32982.0
6	19384.0
7	17604.5
8	15825.0
9	16429.0
10	17033.0
11	17748.0
12	18463.0
13	17896.0
14	17329.0
15	16743.0
16	16157.0
17	15324.0
18	14491.0
19	14831.5
20	15172.0
21	14989.0
22	14806.0
23	16871.5
24	18937.0
25	23285.5
26	32271.5
27	36909.0
28	40972.0
29	45035.0
30	54092.0
31	63149.0
32	72451.5
33	81754.0
34	90707.5
35	99661.0
36	108996.5
37	118332.0
38	129025.0
39	139718.0
40	170376.5
41	201035.0
42	231099.0
43	261163.0
44	274686.5
45	288210.0
46	311230.5
47	334251.0
48	341590.0
49	348929.0
50	330796.0
51	312663.0
52	276839.0
53	241015.0
54	214853.0
55	188691.0
56	177274.0
57	165857.0
58	155586.0
59	145315.0
60	130187.5
61	115060.0
62	102500.0
63	89940.0
64	72704.5
65	55469.0
66	45911.5
67	36354.0
68	29798.5
69	23243.0
70	19079.0
71	14915.0
72	12358.5
73	9802.0
74	7262.5
75	3715.0
76	2707.0
77	2195.5
78	1684.0
79	1227.0
80	770.0
81	645.5
82	521.0
83	335.0
84	149.0
85	113.0
86	77.0
87	62.5
88	48.0
89	37.5
90	27.0
91	22.0
92	17.0
93	12.0
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3674748.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.33912378314085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.33487702038241	16.28919389708774
2	9.232947720313526	3.9404602857407682
3	3.4695534895215383	2.221116941553851
4	1.7399732395962053	1.4851801735638406
5	1.0191390729819483	1.0873767415298605
6	0.734460375965665	0.9403644525926097
7	0.5152384434105701	0.7696315845239677
8	0.39946437002553625	0.6819375711143436
9	0.3134374577595934	0.601963263846445
>10	3.6094273820381972	18.69928714562976
>50	1.8268400487233747	28.744340351655822
>100	0.7982697035835105	21.98345895309343
>500	0.0032508549479071	0.5018531937948879
>1k	0.0029907865520745315	1.2141484213031688
>5k	0.0	0.0
>10k+	1.3003419791628398E-4	0.8396870229695399
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	30261	0.8234850389741011	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7212750370909786E-5	0.0	0.0	0.09867343284491889	0.0
2	2.7212750370909786E-5	0.0	0.0	0.32935591773912115	0.0
3	2.7212750370909786E-5	0.0	0.0	0.6000683584289317	0.0
4	2.7212750370909786E-5	0.0	0.0	0.9646375751480102	0.0
5	2.7212750370909786E-5	0.0	0.0	1.7122806788383858	0.0
6	2.7212750370909786E-5	0.0	0.0	2.797770078383606	0.0
7	2.7212750370909786E-5	0.0	0.0	3.4237994006663857	0.0
8	2.7212750370909786E-5	0.0	0.0	4.78070877241106	0.0
9	2.7212750370909786E-5	0.0	2.7212750370909786E-5	5.491124833594031	0.0
10	2.7212750370909786E-5	0.0	2.7212750370909786E-5	6.467355040400049	0.0
11	2.7212750370909786E-5	0.0	2.7212750370909786E-5	7.355633638007286	0.0
12	2.7212750370909786E-5	0.0	2.7212750370909786E-5	8.036333375785224	0.0
13	5.442550074181957E-5	0.0	2.7212750370909786E-5	8.338775883407516	0.0
14	0.003047828041541896	0.0	2.7212750370909786E-5	8.51628465407696	0.0
15	0.003047828041541896	0.0	2.7212750370909786E-5	8.686119429141808	0.0
16	0.003047828041541896	0.0	2.7212750370909786E-5	8.99667133637463	0.0
17	0.003047828041541896	0.0	2.7212750370909786E-5	9.385296624421594	0.0
18	0.003047828041541896	0.0	2.7212750370909786E-5	9.87063602728677	0.0
19	0.003047828041541896	0.0	2.7212750370909786E-5	10.142382552490675	0.0
20	0.003047828041541896	0.0	2.7212750370909786E-5	10.443736550098128	0.0
21	0.003047828041541896	0.0	2.7212750370909786E-5	10.788848650302008	0.0
22	0.003047828041541896	0.0	2.7212750370909786E-5	11.176086088080053	0.0
23	0.003047828041541896	0.0	2.7212750370909786E-5	11.568058544422637	0.0
24	0.003047828041541896	0.0	2.7212750370909786E-5	11.859234973391374	0.0
25	0.003047828041541896	0.0	2.7212750370909786E-5	12.115579081885343	0.0
26	0.003047828041541896	0.0	2.7212750370909786E-5	12.360820388227982	0.0
27	0.003047828041541896	0.0	2.7212750370909786E-5	12.634362954956368	0.0
28	0.003047828041541896	0.0	2.7212750370909786E-5	12.899932185826076	0.0
29	0.003047828041541896	0.0	2.7212750370909786E-5	13.206429393253632	0.0
30	0.003047828041541896	0.0	2.7212750370909786E-5	13.614375734063941	0.0
31	0.003047828041541896	0.0	2.7212750370909786E-5	13.928628575347208	0.0
32	0.003047828041541896	0.0	2.7212750370909786E-5	14.207967457904596	0.0
33	0.003047828041541896	0.0	2.7212750370909786E-5	14.48923844573832	0.0
34	0.003047828041541896	0.0	2.7212750370909786E-5	14.781367320970036	0.0
35	0.003047828041541896	0.0	2.7212750370909786E-5	15.133962927525914	0.0
36	0.003047828041541896	0.0	2.7212750370909786E-5	15.424649526987974	0.0
37	0.003047828041541896	0.0	2.7212750370909786E-5	15.733731945700766	0.0
38	0.003047828041541896	0.0	2.7212750370909786E-5	16.080531236427642	0.0
39	0.003047828041541896	0.0	2.7212750370909786E-5	16.651590802961184	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	360	0.0	43.75	28
CGTTTTT	13930	0.0	42.657932	1
CGGTCTA	375	0.0	42.0	31
CGCGATT	40	3.460991E-7	39.375	22
CGTTCGA	80	0.0	39.375	14
CTCACGA	420	0.0	39.107143	24
TACGGGA	1250	0.0	38.88	4
TTAGGGA	4640	0.0	38.599136	4
ACGGGAC	1095	0.0	38.21918	5
ATAGGGA	4985	0.0	37.913742	4
TAGGGAC	4055	0.0	37.84217	5
TCGTTAG	125	0.0	37.8	1
ACGGGAT	1690	0.0	37.544376	5
TAAGGGA	4525	0.0	37.44199	4
CGGTTTT	1425	0.0	37.421055	1
AAGGGAT	5890	0.0	37.13073	5
TAGGGAT	5250	0.0	37.071426	5
GGGCGAT	4760	0.0	37.058823	7
GGCGATA	1020	0.0	37.058823	8
GTAGGGA	5120	0.0	36.82617	4
>>END_MODULE