FastQCFastQC Report
Thu 26 May 2016
SRR1547854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547854_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1408292
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT434373.0843745473239927No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43270.307251621112667No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC15490.10999139383025679No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT551.8189894E-1245.0000044
TCGATAG551.8189894E-1245.0000041
CGACGGT1450.045.028
TTTCGCG207.0338283E-445.01
GCGTAAG302.1658525E-644.9999961
CGTTTTT156300.043.877161
CGGTCTA1500.043.531
CTCACGA1550.042.0967724
CTAACGG556.184564E-1140.9090922
CGGTTTT16050.040.794391
TCACGAC1600.040.7812525
CACGACG1600.040.7812526
CGGGATC4250.040.7647066
TACGCGG1050.040.7142872
GCGCGAC3050.040.5737729
TAGGGCG3900.040.3846175
AGGGCGC1850.040.1351366
AATTCGC451.9288564E-840.013
ACGGGAT7450.039.8657725
CGAGGGA7450.039.8657724