##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547854_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1408292
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.515146006652028	31.0	31.0	33.0	30.0	34.0
2	31.85499527086712	31.0	31.0	34.0	30.0	34.0
3	31.494043138780878	31.0	31.0	34.0	30.0	34.0
4	35.51806443550059	37.0	35.0	37.0	33.0	37.0
5	35.61191571066228	37.0	35.0	37.0	33.0	37.0
6	35.73002473918761	37.0	35.0	37.0	33.0	37.0
7	36.04456817194161	37.0	35.0	37.0	35.0	37.0
8	36.09191559704948	37.0	35.0	37.0	35.0	37.0
9	37.87041039784363	39.0	38.0	39.0	35.0	39.0
10	37.49363626293411	39.0	37.0	39.0	35.0	39.0
11	37.21067221854558	39.0	37.0	39.0	34.0	39.0
12	36.79497220746834	39.0	35.0	39.0	33.0	39.0
13	36.66350018320064	39.0	35.0	39.0	33.0	39.0
14	37.83439726988437	40.0	36.0	41.0	33.0	41.0
15	37.9778362725912	40.0	36.0	41.0	33.0	41.0
16	38.04329712872046	40.0	36.0	41.0	33.0	41.0
17	37.9717970420907	40.0	36.0	41.0	33.0	41.0
18	37.758918604948406	39.0	36.0	41.0	33.0	41.0
19	37.54538973451528	39.0	36.0	41.0	33.0	41.0
20	37.24980685823679	39.0	35.0	41.0	33.0	41.0
21	37.14314218926189	39.0	35.0	41.0	33.0	41.0
22	37.0495671352248	38.0	35.0	41.0	33.0	41.0
23	36.95649836823613	38.0	35.0	41.0	32.0	41.0
24	36.92099081724529	38.0	35.0	41.0	33.0	41.0
25	36.86700059362689	38.0	35.0	41.0	32.0	41.0
26	36.76996460961221	38.0	35.0	41.0	32.0	41.0
27	36.68615528597763	38.0	35.0	41.0	32.0	41.0
28	36.60489230926541	38.0	35.0	41.0	32.0	41.0
29	36.56517753420455	38.0	35.0	40.0	32.0	41.0
30	36.44571935365677	38.0	35.0	40.0	31.0	41.0
31	36.21445126436847	38.0	35.0	40.0	31.0	41.0
32	35.868778633976476	38.0	35.0	40.0	30.0	41.0
33	35.55252106807395	38.0	35.0	40.0	29.0	41.0
34	35.200832639821854	38.0	35.0	40.0	25.0	41.0
35	34.90517662530214	38.0	34.0	40.0	24.0	41.0
36	34.70246440368901	38.0	34.0	40.0	23.0	41.0
37	34.540685454437	38.0	34.0	40.0	22.0	41.0
38	34.39349225870771	37.0	34.0	40.0	21.0	41.0
39	34.27686161676698	37.0	34.0	40.0	21.0	41.0
40	34.12422565774712	37.0	34.0	40.0	20.0	41.0
41	34.031436662283106	37.0	34.0	40.0	19.0	41.0
42	33.93771959224365	37.0	33.0	40.0	18.0	41.0
43	33.855108173589	37.0	33.0	40.0	18.0	41.0
44	33.66554166323461	36.0	33.0	40.0	18.0	41.0
45	33.54720682926552	36.0	33.0	40.0	18.0	41.0
46	33.51962945184663	36.0	33.0	40.0	18.0	41.0
47	33.462420435534675	36.0	33.0	40.0	18.0	41.0
48	33.350774555276885	36.0	33.0	40.0	18.0	41.0
49	33.223073765951945	36.0	33.0	40.0	17.0	41.0
50	33.13229997756147	35.0	33.0	40.0	17.0	41.0
51	32.84593535999637	35.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	10.0
14	11.0
15	31.0
16	98.0
17	208.0
18	531.0
19	1067.0
20	1919.0
21	3255.0
22	5221.0
23	8277.0
24	13884.0
25	23109.0
26	31936.0
27	34843.0
28	32864.0
29	31605.0
30	33230.0
31	37580.0
32	45710.0
33	59640.0
34	101236.0
35	165442.0
36	111990.0
37	126392.0
38	188344.0
39	349790.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.305286119639963	22.83382991595493	27.264516165681552	20.596367798723563
2	37.09507687326208	24.00745015948397	27.15800416390919	11.73946880334476
3	26.972957312830008	23.226646178491393	37.57303172921525	12.22736477946335
4	23.065884063816313	25.501742536349	37.815950101257414	13.616423298577285
5	21.20156899279411	29.879243793190618	35.99743519099732	12.921752023017955
6	18.95203551536187	37.64631198643463	33.27626656971708	10.125385928486422
7	80.90530941026435	3.1212276999372293	14.296182893888485	1.6772799959099391
8	81.47415450772993	2.5484771624066602	13.973522536519415	2.0038457933439937
9	77.16318774799544	4.974252498771562	15.395315744178054	2.4672440090549403
10	38.77377702919565	32.88778179525269	19.535437253069677	8.803003922481984
11	28.36556623200302	23.26896694719561	34.284722202497775	14.080744618303589
12	27.43188202446652	22.318027795371982	34.67540822499879	15.574681955162708
13	20.249280688948033	29.542381835585235	34.987275366188264	15.221062109278474
14	15.324591775001206	33.327037290561904	35.29942653938246	16.048944395054434
15	14.23689121290187	27.165104964027346	43.67070181468048	14.927302008390306
16	17.220363390546847	25.435634087248953	42.11413542077921	15.229867101424988
17	16.91723023350271	25.162395298702258	34.75848758638123	23.1618868814138
18	19.087660797618675	26.75638290922621	37.02506298409705	17.130893309058067
19	21.580467687098984	28.52022165857649	32.779636609453156	17.119674044871378
20	22.864789404470095	28.344618871654458	32.21171461600293	16.578877107872515
21	20.025037421216624	28.862125184265764	34.67320697696216	16.43963041755545
22	19.863423210527362	25.978987312290347	34.030229526263014	20.127359950919267
23	16.51134849874884	30.347257528978368	33.62988641560131	19.511507556671486
24	17.088004476344395	28.26828527038427	38.453175903860846	16.19053434941049
25	16.972332442419614	29.271841351083438	35.98621592681063	17.769610279686315
26	15.798073126879936	32.71090086430939	33.1590323597663	18.331993649044374
27	15.881933576275376	31.32581879326162	35.24176804242302	17.55047958803998
28	14.853382679160287	30.099723636859398	38.93666938390618	16.110224300074133
29	15.387078816040992	27.895990320189277	38.38770652677144	18.329224336998294
30	17.150633533386543	29.36919332070338	36.78512694810451	16.695046197805567
31	21.507755493889054	28.75646527850758	32.61894550277925	17.11683372482411
32	21.421480772453442	29.42067412156002	32.67312460768079	16.484720498305748
33	20.35352043468258	29.70562923030167	32.23358508036686	17.707265254648892
34	17.13330758109824	30.771956384045357	33.590832014951445	18.50390401990496
35	17.63938160551931	29.915244849789673	33.616962959386264	18.828410585304752
36	22.17217736094503	29.967364722656946	30.671835102379337	17.188622814018682
37	18.862352409869544	32.00266706052438	31.60580334192057	17.52917718768551
38	19.042854748873104	32.77771939342125	29.149352549045226	19.03007330866042
39	19.039801404822295	31.70251623952987	30.06997128436432	19.187711071283513
40	20.994296637345098	29.637603565169723	30.110587861040184	19.257511936445
41	17.79332695208096	28.526257338676924	31.188773350981187	22.49164235826093
42	19.84389601020243	30.074515796439943	29.547281387666764	20.534306805690868
43	19.87414541870578	29.852189744740436	30.098800532844038	20.174864303709743
44	18.85312136971594	32.506184796902915	29.9806432188779	18.66005061450324
45	17.568373604337737	33.8811837317829	28.21410616548273	20.33633649839664
46	20.01999585313273	31.90574113891153	29.488202730683692	18.586060277272043
47	18.934354523067658	30.543736668247778	30.697539998807066	19.824368809877495
48	19.99642119674045	29.023384354949116	31.4789120438091	19.50128240450134
49	19.36544409824099	28.123641971977403	31.375453386087543	21.135460543694062
50	17.920431274195977	30.902043042209996	31.762802032533028	19.414723651061003
51	17.310543552047445	32.137014198759914	30.07479982844467	20.477642420747973
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	824.0
1	1358.5
2	1893.0
3	29396.0
4	56899.0
5	38415.0
6	19931.0
7	18124.0
8	16317.0
9	18241.0
10	20165.0
11	21701.5
12	23238.0
13	23180.0
14	23122.0
15	21924.5
16	20727.0
17	19012.5
18	17298.0
19	15873.0
20	14448.0
21	14014.0
22	13580.0
23	13241.0
24	12902.0
25	13613.0
26	16725.0
27	19126.0
28	21079.0
29	23032.0
30	26375.5
31	29719.0
32	32944.5
33	36170.0
34	39924.5
35	43679.0
36	47004.5
37	50330.0
38	55484.5
39	60639.0
40	69459.5
41	78280.0
42	89388.0
43	100496.0
44	103332.5
45	106169.0
46	110665.0
47	115161.0
48	112495.5
49	109830.0
50	102341.5
51	94853.0
52	84515.0
53	74177.0
54	64650.5
55	55124.0
56	49128.0
57	43132.0
58	38855.5
59	34579.0
60	30814.0
61	27049.0
62	22854.5
63	18660.0
64	15645.0
65	12630.0
66	10053.0
67	7476.0
68	5901.5
69	4327.0
70	3690.5
71	3054.0
72	2670.5
73	2287.0
74	1782.5
75	983.0
76	688.0
77	465.5
78	243.0
79	216.0
80	189.0
81	139.5
82	90.0
83	76.0
84	62.0
85	51.0
86	40.0
87	31.0
88	22.0
89	17.5
90	13.0
91	11.0
92	9.0
93	9.0
94	9.0
95	5.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1408292.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.988082388438364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62297861011207	21.63177052020602
2	8.498125747336664	4.926887386222091
3	3.593564980305615	3.1251167315191815
4	2.0136783940667646	2.3349070076410245
5	1.2689826963985193	1.839268747635147
6	0.9040369264124125	1.572377814302016
7	0.7369023354570337	1.4952969928722795
8	0.6024488444691213	1.3971069390632298
9	0.4983989344955588	1.3002866437920435
>10	6.751298057778767	46.10219848678179
>50	0.4681784015033116	8.18273760524705
>100	0.03719108240105066	1.817889106535831
>500	0.0029752865386619724	0.5615453852501877
>1k	9.917621795539908E-4	0.590654035067969
>5k	0.0	0.0
>10k+	2.479405448884977E-4	3.121956597864124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43437	3.0843745473239927	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4327	0.307251621112667	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1549	0.10999139383025679	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05240390487200098	0.0
2	0.0	0.0	0.0	0.1628923547105288	0.0
3	0.0	0.0	0.0	0.3157015732532742	0.0
4	0.0	0.0	0.0	0.47319731987400343	0.0
5	0.0	0.0	0.0	0.8528060941906934	0.0
6	0.0	0.0	0.0	1.597964058590122	0.0
7	0.0	0.0	0.0	2.135068579527541	0.0
8	0.0	0.0	0.0	3.073794355147938	0.0
9	0.0	0.0	0.0	3.703422301624947	0.0
10	0.0	0.0	0.0	4.396105353151193	0.0
11	0.0	0.0	0.0	4.886486609311137	0.0
12	0.0	0.0	0.0	5.279089847844055	0.0
13	0.0	0.0	0.0	5.474361851093382	0.0
14	0.0	0.0	0.0	5.587193564970901	0.0
15	0.0	0.0	0.0	5.688024926648735	0.0
16	0.0	0.0	0.0	5.845733697273008	0.0
17	0.0	0.0	0.0	6.032129700374639	0.0
18	0.0	0.0	0.0	6.313960457064302	0.0
19	0.0	0.0	0.0	6.453988235394364	0.0
20	0.0	0.0	0.0	6.604383181896936	0.0
21	0.0	0.0	0.0	6.763299088541297	0.0
22	0.0	0.0	0.0	6.938333811453875	0.0
23	0.0	0.0	0.0	7.135452022733921	0.0
24	0.0	0.0	0.0	7.289894425303843	0.0
25	0.0	0.0	0.0	7.416856731416496	0.0
26	0.0	0.0	0.0	7.544458109539783	0.0
27	0.0	0.0	0.0	7.6848409278757535	0.0
28	0.0	0.0	0.0	7.826714914236536	0.0
29	0.0	0.0	0.0	7.9840686448549025	0.0
30	0.0	0.0	0.0	8.215270696702104	0.0
31	0.0	0.0	0.0	8.381713451471711	0.0
32	0.0	0.0	0.0	8.547020078222413	0.0
33	0.0	0.0	0.0	8.712326704973117	0.0
34	0.0	0.0	0.0	8.885444211853791	0.0
35	0.0	0.0	0.0	9.084550647166923	0.0
36	0.0	0.0	0.0	9.25781017004996	0.0
37	0.0	0.0	0.0	9.433199932968447	0.0
38	0.0	0.0	0.0	9.63287443229103	0.0
39	0.0	0.0	0.0	9.892266660607318	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	55	1.8189894E-12	45.000004	4
TCGATAG	55	1.8189894E-12	45.000004	1
CGACGGT	145	0.0	45.0	28
TTTCGCG	20	7.0338283E-4	45.0	1
GCGTAAG	30	2.1658525E-6	44.999996	1
CGTTTTT	15630	0.0	43.87716	1
CGGTCTA	150	0.0	43.5	31
CTCACGA	155	0.0	42.09677	24
CTAACGG	55	6.184564E-11	40.909092	2
CGGTTTT	1605	0.0	40.79439	1
TCACGAC	160	0.0	40.78125	25
CACGACG	160	0.0	40.78125	26
CGGGATC	425	0.0	40.764706	6
TACGCGG	105	0.0	40.714287	2
GCGCGAC	305	0.0	40.573772	9
TAGGGCG	390	0.0	40.384617	5
AGGGCGC	185	0.0	40.135136	6
AATTCGC	45	1.9288564E-8	40.0	13
ACGGGAT	745	0.0	39.865772	5
CGAGGGA	745	0.0	39.865772	4
>>END_MODULE