FastQCFastQC Report
Thu 26 May 2016
SRR1547853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547853_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2877451
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184710.6419223124911597No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC36530.12695263968005016No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG1400.043.392861
CGGTCTA5600.042.58928731
GCGATAT2250.042.09
CGTTTTT82850.041.8225751
TCACGAC6400.041.48437525
CGACGGT5850.040.38461728
CGGGTAT2150.039.767446
TAGTACG1250.039.61
CGCATCG3250.039.4615421
CACGACG6250.039.2426
ATAGGGC10500.039.2142874
AGGGCGA19950.038.7969936
CGTTAGG2800.038.571432
GCGATCG700.038.571439
GGGCGAT38450.038.270487
ACGAGGG12500.038.163
ACGGTCT6300.037.85714330
CTCACGA6800.037.7205924
GGCGATT11700.037.692318
CGTAAGG4250.037.5882382
Produced by FastQC (version 0.11.5)