FastQCFastQC Report
Thu 26 May 2016
SRR1547852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547852_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3342736
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271970.813614954935119No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33800.10111477544143481No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG207.03522E-445.00000430
CGTTTTT125900.042.837571
CGACGGT4250.040.76470628
GCGCGAC9750.039.6923079
CGCGTAA1600.039.37500431
TACGGGA15650.039.105434
AGGGCGA27150.038.6187866
CGAATCG356.2518684E-638.57142636
ACGGGAT18350.038.3787465
TAGGGAC44600.038.0885665
CGCGACC10150.037.90640310
GGGCGAT48900.037.5460137
GCGATCG601.5643309E-1037.5000049
TGCGTAC301.1403879E-437.50000439
CGCCGGT9200.037.4184828
TAGACGG4400.037.3295442
ATAGGGA43350.037.318344
CACGCCG9500.037.18420826
GACCGAT11450.037.1397369
TCTAGCG1700.037.0588231
Produced by FastQC (version 0.11.5)