##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547852_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3342736
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.531857436543	31.0	31.0	33.0	30.0	34.0
2	31.94454303301248	31.0	31.0	34.0	30.0	34.0
3	31.954299711374155	33.0	31.0	34.0	30.0	34.0
4	35.77077579563567	37.0	35.0	37.0	35.0	37.0
5	35.72627990963091	37.0	35.0	37.0	33.0	37.0
6	35.776725113798996	37.0	35.0	37.0	35.0	37.0
7	36.06237944007543	37.0	35.0	37.0	35.0	37.0
8	36.09748541314659	37.0	35.0	37.0	35.0	37.0
9	37.87741149764744	39.0	38.0	39.0	35.0	39.0
10	37.426390836727755	39.0	37.0	39.0	35.0	39.0
11	37.11089748038732	39.0	37.0	39.0	33.0	39.0
12	36.73459764695746	39.0	35.0	39.0	33.0	39.0
13	36.60654894673106	39.0	35.0	39.0	33.0	39.0
14	37.705846348619815	40.0	36.0	41.0	33.0	41.0
15	37.86168575681717	40.0	36.0	41.0	33.0	41.0
16	37.92286169173994	40.0	36.0	41.0	33.0	41.0
17	37.84638780926762	40.0	36.0	41.0	33.0	41.0
18	37.73713060199788	39.0	36.0	41.0	33.0	41.0
19	37.65794726236233	39.0	36.0	41.0	33.0	41.0
20	37.505719267091386	39.0	35.0	41.0	33.0	41.0
21	37.38942231752672	39.0	35.0	41.0	33.0	41.0
22	37.33080207351104	39.0	35.0	41.0	32.0	41.0
23	37.22717767720813	39.0	35.0	41.0	32.0	41.0
24	37.259752490175714	39.0	35.0	41.0	32.0	41.0
25	37.22244233466238	39.0	35.0	41.0	32.0	41.0
26	37.11833869022262	39.0	35.0	41.0	32.0	41.0
27	37.06088814671575	39.0	35.0	41.0	32.0	41.0
28	36.99843421676136	39.0	35.0	41.0	32.0	41.0
29	36.99846233743855	39.0	35.0	41.0	32.0	41.0
30	36.90100265172003	39.0	35.0	41.0	31.0	41.0
31	36.75859954241077	39.0	35.0	41.0	31.0	41.0
32	36.618094877968225	39.0	35.0	41.0	31.0	41.0
33	36.50129385030706	39.0	35.0	41.0	30.0	41.0
34	36.33041735871454	39.0	35.0	41.0	30.0	41.0
35	36.225657964015106	39.0	35.0	41.0	30.0	41.0
36	36.10856196839954	39.0	35.0	41.0	30.0	41.0
37	36.015767921846056	39.0	35.0	41.0	29.0	41.0
38	35.904278710613106	38.0	35.0	41.0	29.0	41.0
39	35.832362172783014	38.0	35.0	41.0	29.0	41.0
40	35.71000761053221	38.0	35.0	40.0	28.0	41.0
41	35.6451044892567	38.0	35.0	40.0	28.0	41.0
42	35.582979631056716	38.0	35.0	40.0	27.0	41.0
43	35.538405066987046	38.0	35.0	40.0	27.0	41.0
44	35.37745487528779	38.0	34.0	40.0	27.0	41.0
45	35.24534812201741	38.0	34.0	40.0	26.0	41.0
46	35.23412019375745	38.0	34.0	40.0	27.0	41.0
47	35.158935674250074	38.0	34.0	40.0	26.0	41.0
48	35.07230005600203	38.0	34.0	40.0	26.0	41.0
49	34.9558840422935	37.0	34.0	40.0	26.0	41.0
50	34.874014579673656	37.0	34.0	40.0	26.0	41.0
51	34.56587747282465	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	7.0
12	10.0
13	12.0
14	35.0
15	77.0
16	200.0
17	461.0
18	981.0
19	1934.0
20	3634.0
21	5764.0
22	9069.0
23	13768.0
24	21242.0
25	32541.0
26	44423.0
27	50703.0
28	54244.0
29	59250.0
30	69229.0
31	84073.0
32	105852.0
33	138474.0
34	234942.0
35	343241.0
36	235480.0
37	317983.0
38	522268.0
39	992695.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.65411926039029	24.042431110323996	29.24508546292618	14.058364166359533
2	31.946315832300247	25.35749158772933	29.87334327329469	12.82284930667573
3	28.97019088555004	25.265740399481146	32.39609708933042	13.3679716256384
4	25.355846228957358	27.688306824110548	31.90266895142183	15.053177995510264
5	23.03577069801504	31.68111391387175	30.868635752269995	14.414479635843215
6	21.226055542525643	41.00892203273008	27.419694525681958	10.345327899062324
7	88.91575643425027	2.9541070548197643	6.758565438610767	1.3715710723192018
8	89.9319599274367	2.191468306201866	6.321169245791472	1.5554025205699762
9	85.17854835081204	4.81207609574911	7.763700154603893	2.2456753988349663
10	43.02981150769909	34.00268522551587	13.211333470546283	9.75616979623877
11	29.31472901240182	24.663778413850213	28.448851479745933	17.57264109400204
12	29.928477749962905	22.801740849411978	29.663365578376517	17.6064158222486
13	23.56318297346844	28.19355162956333	29.74335993030859	18.499905466659648
14	17.95777470910057	32.36241210792596	30.856131025602977	18.823682157370488
15	17.535814973123813	27.46489701849024	37.724038033515065	17.275249974870885
16	22.055436026057695	24.391007845070625	35.57367378099856	17.97988234787312
17	21.199071658665236	24.17244436892414	29.84953044452209	24.778953527888532
18	22.156939704481598	26.188427683191257	32.534785876000974	19.11984673632617
19	25.30190837685058	27.45008878954246	27.993984568329655	19.254018265277306
20	26.281465242842987	27.259705821817814	27.853082026220438	18.605746909118757
21	23.84714198189746	26.320564950387947	31.41199305000455	18.42030001771004
22	23.336243125391896	23.564409513643913	30.268827690849655	22.83051967011454
23	19.863638648101436	27.915844984467814	30.221202033304458	21.999314334126296
24	20.120524025827944	27.063668803040386	33.87066762077532	18.945139550356355
25	21.41524188568885	27.22617041848354	31.184754045787642	20.173833650039967
26	19.32055059089321	31.013935889642497	28.228911885353792	21.4366016341105
27	19.120714289133215	29.745124951536706	31.285689327544862	19.84847143178522
28	18.05075243752423	28.17739719798393	34.26707942236539	19.504770942126452
29	19.451102330545996	26.576852015833737	33.14958166005332	20.82246399356695
30	20.60985372461361	27.362047137434725	32.48656190617506	19.541537231776605
31	24.626114655778977	26.73639198548734	28.55771439922267	20.07977895951101
32	24.680561073324366	27.48138052182404	28.648029637997137	19.190028766854457
33	24.345476280507945	26.97481942935368	28.051213137980387	20.628491152157995
34	20.362870415132996	27.60005576270456	30.534568090330794	21.50250573183165
35	20.7938646665486	27.066241545847475	30.40898234260797	21.730911444995957
36	25.106409839125792	26.728793419522212	28.89285902326717	19.271937718084825
37	21.4950567439367	29.000166330814043	29.413899272930916	20.09087765231834
38	21.20388807252502	30.37769061032639	27.403988828313093	21.014432488835492
39	20.823361461988025	27.90241287376568	28.955472403444364	22.31875326080193
40	22.30502199395944	26.814142666366713	29.312156269594723	21.56867907007912
41	19.44452089545809	26.284875622843085	30.434919179977122	23.835684301721706
42	21.335127871300635	27.71555994849728	28.28551222710977	22.66379995309232
43	21.224589677437883	27.244807846027925	29.176010310117224	22.35459216641697
44	20.919510245499495	28.357608856936356	29.236858669066297	21.486022228497852
45	19.956047979858415	29.978526572245013	28.183440152019184	21.88198529587739
46	22.630025224845753	28.697420316770454	28.379776326936977	20.292778131446816
47	20.733674451108314	27.97842844903097	29.585644813111177	21.702252286749538
48	21.23945773761374	27.64223677849522	29.863530951890905	21.254774532000134
49	21.759480856400266	26.20263759985832	30.247138870673606	21.79074267306781
50	20.223553400567678	28.177277535527782	30.16116139593435	21.43800766797019
51	19.437012076335076	29.142684316081198	28.91122721028523	22.5090763972985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1292.0
1	2893.5
2	4495.0
3	23573.0
4	42651.0
5	30901.5
6	19152.0
7	17565.0
8	15978.0
9	16408.0
10	16838.0
11	16922.0
12	17006.0
13	16757.5
14	16509.0
15	15600.0
16	14691.0
17	14043.0
18	13395.0
19	13503.0
20	13611.0
21	15243.5
22	16876.0
23	17885.0
24	18894.0
25	23510.5
26	31807.5
27	35488.0
28	44657.5
29	53827.0
30	59933.5
31	66040.0
32	77750.5
33	89461.0
34	101181.5
35	112902.0
36	122744.0
37	132586.0
38	144648.5
39	156711.0
40	178755.5
41	200800.0
42	220574.5
43	240349.0
44	251828.5
45	263308.0
46	272607.0
47	281906.0
48	282039.5
49	282173.0
50	266049.0
51	249925.0
52	230539.5
53	211154.0
54	189906.0
55	168658.0
56	156634.5
57	144611.0
58	133496.5
59	122382.0
60	107198.0
61	92014.0
62	81237.0
63	70460.0
64	58557.5
65	46655.0
66	38324.5
67	29994.0
68	25094.5
69	20195.0
70	16753.5
71	13312.0
72	11340.5
73	9369.0
74	7060.5
75	3298.0
76	1844.0
77	1406.5
78	969.0
79	782.0
80	595.0
81	508.5
82	422.0
83	291.0
84	160.0
85	136.0
86	112.0
87	79.5
88	47.0
89	35.5
90	24.0
91	16.0
92	8.0
93	7.0
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3342736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.314040484503597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.23721383978118	13.962114206875102
2	8.544160148199447	3.1295618972041384
3	2.96446934473694	1.6287423478374652
4	1.5824004614614382	1.159205844556078
5	0.9800575442956394	0.8974406771686758
6	0.6894985225699706	0.7576502313811114
7	0.5173139108115219	0.6631875534059365
8	0.4090506650442251	0.5993096351866446
9	0.3499996453618692	0.5768916907247257
>10	4.686369076720368	21.565466011228406
>50	1.9287701756593718	25.147605011433953
>100	1.1031923024622918	27.69498716388953
>500	0.005002908597248366	0.643685422053564
>1k	0.002334690678715904	0.7435244638401033
>5k	0.0	0.0
>10k+	1.6676361990827887E-4	0.8306278432145939
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27197	0.813614954935119	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	3380	0.10111477544143481	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041911775264334365	0.0
2	0.0	0.0	0.0	0.12118815245954212	0.0
3	0.0	0.0	0.0	0.19475064737388773	0.0
4	0.0	0.0	0.0	0.3020280393067236	0.0
5	0.0	0.0	0.0	0.5337244700149817	0.0
6	0.0	0.0	0.0	0.8871176186213928	0.0
7	0.0	0.0	0.0	1.0838726121356876	0.0
8	0.0	0.0	0.0	1.562073702499988	0.0
9	0.0	0.0	0.0	1.7955351544363658	0.0
10	0.0	0.0	0.0	2.1572448437447647	0.0
11	0.0	0.0	0.0	2.54770343814169	0.0
12	0.0	0.0	0.0	2.8475476376237907	0.0
13	0.0	0.0	0.0	2.981061023066135	0.0
14	0.0	0.0	0.0	3.0451103527170558	0.0
15	0.0	0.0	0.0	3.123728586403473	0.0
16	0.0	0.0	0.0	3.279678682372763	0.0
17	0.0	0.0	0.0	3.4844510604486865	0.0
18	0.0	0.0	0.0	3.744746818175291	0.0
19	0.0	0.0	0.0	3.897705352740988	0.0
20	0.0	0.0	0.0	4.056826503798086	0.0
21	0.0	0.0	0.0	4.2419443234524055	0.0
22	0.0	0.0	0.0	4.453447714686413	0.0
23	0.0	0.0	0.0	4.683109883640228	0.0
24	0.0	0.0	0.0	4.859970993820631	0.0
25	0.0	0.0	0.0	5.011553410140675	0.0
26	0.0	0.0	0.0	5.157451859793893	0.0
27	0.0	0.0	0.0	5.31570545804395	0.0
28	0.0	0.0	0.0	5.48407053383815	0.0
29	0.0	0.0	0.0	5.681304177177019	0.0
30	0.0	0.0	0.0	5.9101885401659	0.0
31	0.0	0.0	0.0	6.109366698417105	0.0
32	0.0	0.0	0.0	6.298104307369772	0.0
33	0.0	0.0	0.0	6.490282211936569	0.0
34	0.0	0.0	0.0	6.695892227205499	0.0
35	0.0	0.0	0.0	6.930699881773493	0.0
36	0.0	0.0	0.0	7.139690361428483	0.0
37	0.0	0.0	0.0	7.352869027048501	0.0
38	0.0	0.0	0.0	7.591535795827131	0.0
39	0.0	0.0	0.0	7.94002278373165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTACCG	20	7.03522E-4	45.000004	30
CGTTTTT	12590	0.0	42.83757	1
CGACGGT	425	0.0	40.764706	28
GCGCGAC	975	0.0	39.692307	9
CGCGTAA	160	0.0	39.375004	31
TACGGGA	1565	0.0	39.10543	4
AGGGCGA	2715	0.0	38.618786	6
CGAATCG	35	6.2518684E-6	38.571426	36
ACGGGAT	1835	0.0	38.378746	5
TAGGGAC	4460	0.0	38.088566	5
CGCGACC	1015	0.0	37.906403	10
GGGCGAT	4890	0.0	37.546013	7
GCGATCG	60	1.5643309E-10	37.500004	9
TGCGTAC	30	1.1403879E-4	37.500004	39
CGCCGGT	920	0.0	37.41848	28
TAGACGG	440	0.0	37.329544	2
ATAGGGA	4335	0.0	37.31834	4
CACGCCG	950	0.0	37.184208	26
GACCGAT	1145	0.0	37.139736	9
TCTAGCG	170	0.0	37.058823	1
>>END_MODULE