FastQCFastQC Report
Thu 26 May 2016
SRR1547851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547851_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1488207
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72720.4886417010536841No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC31210.20971544953087845No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG22590.15179339970850828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG351.2122473E-745.00000442
TTTACGA207.033957E-445.00000414
CGCGAGA207.033957E-445.00000420
CGCTAAC207.033957E-445.00000431
CCATACG351.2122473E-745.0000041
TATTACG207.033957E-445.0000041
TCCGTCG207.033957E-445.00000414
GTAACGT207.033957E-445.00000424
CGCCCGA207.033957E-445.00000441
TGCCGAT207.033957E-445.00000438
ACGCAAC253.8913626E-545.044
TTACGTA253.8913626E-545.043
TCGAACA253.8913626E-545.037
AACACGA253.8913626E-545.041
CGAGTAC302.1659362E-644.9999969
ACGATTC302.1659362E-644.99999610
ACTACGG600.044.9999962
TTTACGG1650.043.6363642
CGTAAGG1800.042.52
CGTTTTT33700.042.0623131