##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547851_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1488207
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50206725274105	31.0	31.0	33.0	30.0	34.0
2	31.91858390667427	31.0	31.0	34.0	30.0	34.0
3	31.97477165474964	33.0	31.0	34.0	30.0	34.0
4	35.73068598655966	37.0	35.0	37.0	35.0	37.0
5	35.6250467844863	37.0	35.0	37.0	33.0	37.0
6	35.66802534862422	37.0	35.0	37.0	33.0	37.0
7	36.01058522100756	37.0	35.0	37.0	35.0	37.0
8	36.04537406422628	37.0	35.0	37.0	35.0	37.0
9	37.82829807950104	39.0	38.0	39.0	35.0	39.0
10	37.33591093174538	39.0	37.0	39.0	34.0	39.0
11	37.009172111137765	39.0	37.0	39.0	33.0	39.0
12	35.97829938980263	37.0	35.0	39.0	32.0	39.0
13	35.53938128230817	37.0	35.0	39.0	31.0	39.0
14	36.23208196171635	38.0	35.0	41.0	30.0	41.0
15	36.64153306630059	38.0	35.0	41.0	31.0	41.0
16	36.88989502132432	38.0	35.0	41.0	32.0	41.0
17	36.86351898627006	38.0	35.0	41.0	32.0	41.0
18	36.8170355333633	38.0	35.0	41.0	32.0	41.0
19	36.73586806136512	38.0	35.0	41.0	32.0	41.0
20	36.54390417462087	38.0	35.0	40.0	31.0	41.0
21	36.40038516147283	38.0	35.0	40.0	31.0	41.0
22	36.35806376397907	37.0	35.0	40.0	31.0	41.0
23	36.34106344077134	37.0	35.0	40.0	31.0	41.0
24	36.373255870991066	37.0	35.0	40.0	31.0	41.0
25	36.334696046988086	37.0	35.0	40.0	31.0	41.0
26	36.22215121955481	37.0	35.0	40.0	31.0	41.0
27	36.13022113187211	37.0	35.0	40.0	31.0	41.0
28	36.10851648997754	37.0	35.0	40.0	31.0	41.0
29	36.12750847160375	37.0	35.0	40.0	31.0	41.0
30	36.04012546641697	37.0	35.0	40.0	31.0	41.0
31	35.81286272675777	36.0	35.0	40.0	30.0	41.0
32	35.66609013396658	36.0	34.0	40.0	30.0	41.0
33	35.58471906125963	36.0	34.0	40.0	30.0	41.0
34	35.43598639167804	36.0	34.0	40.0	29.0	41.0
35	35.39808910991549	36.0	34.0	40.0	29.0	41.0
36	35.22254834172934	36.0	34.0	40.0	29.0	41.0
37	35.07925443167516	36.0	34.0	40.0	27.0	41.0
38	34.953112033473836	36.0	34.0	40.0	27.0	41.0
39	34.90208956146557	36.0	34.0	40.0	27.0	41.0
40	34.70707367993834	36.0	34.0	40.0	25.0	41.0
41	34.65348704850871	36.0	34.0	40.0	25.0	41.0
42	34.584779536717676	36.0	34.0	40.0	25.0	41.0
43	34.56292773787518	35.0	34.0	40.0	25.0	41.0
44	34.39448678846424	35.0	34.0	40.0	24.0	41.0
45	34.26233581753076	35.0	34.0	40.0	24.0	41.0
46	34.28691841927904	35.0	34.0	40.0	24.0	41.0
47	34.20219297449884	35.0	33.0	40.0	24.0	41.0
48	34.11345263125358	35.0	33.0	40.0	23.0	41.0
49	34.03800882538518	35.0	33.0	40.0	24.0	41.0
50	33.94254965875043	35.0	33.0	39.0	24.0	41.0
51	33.596022596318925	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	2.0
13	6.0
14	13.0
15	61.0
16	146.0
17	364.0
18	738.0
19	1404.0
20	2387.0
21	3942.0
22	5719.0
23	8621.0
24	12347.0
25	17282.0
26	22114.0
27	25563.0
28	28300.0
29	32132.0
30	38318.0
31	45988.0
32	57558.0
33	77968.0
34	149805.0
35	240229.0
36	89809.0
37	115774.0
38	181980.0
39	329595.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.07779092559032	23.97442022514341	29.730675907316655	13.217112941949608
2	31.339188701571757	25.987648223667808	29.319509987521897	13.353653087238538
3	29.910959967262617	24.89680535033097	31.166027306685155	14.026207375721253
4	26.239629298881134	27.768045708695094	30.73517326554707	15.257151726876705
5	23.37383173174162	31.351418183088775	30.4999237337279	14.7748263514417
6	20.809941090184363	41.102548234217416	27.388461416993735	10.699049258604482
7	88.48789180537385	4.06663857917615	5.885068407822299	1.5604012076277023
8	89.65990618240608	2.7392694699057323	5.423035908311142	2.177788439377049
9	85.82885310981604	4.31962757869033	7.234544656758099	2.6169746547355306
10	53.240644614626866	21.95837003857662	13.089912895181921	11.711072451614594
11	46.24665789100575	21.084566864690192	19.219369348484452	13.449405895819599
12	42.185865272774556	20.54143005643704	23.041082322553248	14.23162234823516
13	24.00479234407579	37.88901678328351	23.596650197183592	14.50954067545711
14	14.96901976673944	40.697833029948114	29.87837041486836	14.454776788444082
15	14.59440790158896	24.258386098170483	47.31284021644838	13.834365783792174
16	19.736165735008637	19.719501386567863	43.52707654244335	17.017256335980143
17	20.04788312378587	19.63322306641482	27.26730891603117	33.05158489376814
18	23.747973232218367	23.545582032607022	33.03088884812395	19.67555588705066
19	29.64278490828225	25.53959227446182	25.56223697375432	19.25538584350161
20	29.87790005019463	26.671760044133645	24.55941948935867	18.890920416313055
21	22.669830205072277	27.8848305376873	30.336774386896447	19.108564870343976
22	21.796363005952802	23.662635641412788	29.431725559683564	25.109275792950847
23	18.470817567717393	29.46028341487441	27.882478714318644	24.186420303089555
24	20.109971260718435	26.82355344384215	35.031148220644035	18.035327074795372
25	19.76983040665714	25.463460392270697	33.368610683863196	21.398098517208965
26	16.796924083813607	33.81095506203102	27.16994342856874	22.22217742558663
27	18.35033701628873	32.24531264803888	31.211518290130336	18.19283204554205
28	15.388114691034243	28.54730558316148	37.21908309798301	18.845496627821266
29	17.504016578338902	26.514120683480186	35.83936912002161	20.142493618159303
30	21.063467649325666	28.569345527873473	31.747666823230908	18.619519999569953
31	28.971305739053776	25.69494700670001	25.897539791171525	19.43620746307469
32	30.233562938489065	26.929990250012263	25.52534694434309	17.311099867155576
33	28.44288462559308	26.741575600706085	25.318453682854603	19.49708609084623
34	19.59021829624508	26.854731902215217	30.63088669788544	22.924163103654262
35	19.84367766043299	27.75057502081364	29.811578631198483	22.594168687554887
36	30.507919933181338	24.049073818359943	27.299629688611866	18.14337655984685
37	21.268076282398887	30.45369360579543	29.230745454093416	19.047484657712268
38	21.42040724173452	32.07571258568196	24.154973064902936	22.348907107680585
39	20.6963144240015	29.51713034544254	27.27174378295493	22.514811447601037
40	24.599198901765682	25.169952835862215	26.461843009742598	23.76900525262951
41	18.869149251414623	23.819603052532344	27.64131602660114	29.6699316694519
42	22.175140958213476	26.07950372495224	26.390683554102353	25.354671762731932
43	21.90521883044496	25.712014524861125	27.511764156464793	24.871002488229124
44	21.3140376305178	28.421180655648037	28.750973486887244	21.513808226946924
45	18.550645172344975	33.27359701976943	24.075548629995694	24.1002091778899
46	23.182326114579492	29.90168706369477	26.541737809323568	20.374249012402174
47	21.237368188699556	27.45310296215513	28.442548650826126	22.866980198319187
48	23.204030084524533	25.53703886623299	29.676382384977355	21.58254866426512
49	22.126021447285225	22.837212833967317	31.295511981868113	23.741253736879344
50	19.598147300745126	30.221871016599167	27.765089130745924	22.41489255190978
51	18.489296179899707	33.76761431709433	24.126549599618873	23.616539903387096
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	521.0
1	1170.0
2	1819.0
3	6295.0
4	10771.0
5	7922.5
6	5074.0
7	4858.0
8	4642.0
9	5097.0
10	5552.0
11	5748.0
12	5944.0
13	5962.0
14	5980.0
15	5571.0
16	5162.0
17	5065.5
18	4969.0
19	5125.0
20	5281.0
21	5760.0
22	6239.0
23	6462.0
24	6685.0
25	7211.5
26	10225.5
27	12713.0
28	13736.0
29	14759.0
30	17842.0
31	20925.0
32	23698.5
33	26472.0
34	31161.5
35	35851.0
36	37254.0
37	38657.0
38	47680.0
39	56703.0
40	76715.5
41	96728.0
42	118873.5
43	141019.0
44	143654.5
45	146290.0
46	143842.5
47	141395.0
48	139398.5
49	137402.0
50	126344.0
51	115286.0
52	105709.5
53	96133.0
54	86849.5
55	77566.0
56	75491.0
57	73416.0
58	65809.0
59	58202.0
60	49689.0
61	41176.0
62	35522.5
63	29869.0
64	24663.0
65	19457.0
66	15769.0
67	12081.0
68	9709.5
69	7338.0
70	6004.5
71	4671.0
72	4378.0
73	4085.0
74	3023.0
75	1470.0
76	979.0
77	664.0
78	349.0
79	240.5
80	132.0
81	114.0
82	96.0
83	87.5
84	79.0
85	49.5
86	20.0
87	17.0
88	14.0
89	9.5
90	5.0
91	2.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1488207.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.001429908320002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.85603981528067	15.572321233047075
2	8.870295646152872	3.548371932652055
3	3.120823957549617	1.8726282492940352
4	1.5340342269932412	1.227315122726767
5	0.8859199133574784	0.8859832525702266
6	0.5988364103156415	0.7186550692486957
7	0.4283423318179311	0.5997221388635887
8	0.3506487889942123	0.5610781740404022
9	0.2708621252275114	0.4875866831300998
>10	2.9728354748248105	14.407470938180852
>50	2.0044053386430267	29.917862660081983
>100	1.1025417292414794	28.831065572743636
>500	0.0027164563701667526	0.36572908491744344
>1k	0.0013582281850833763	0.5103228122692052
>5k	3.395570462708441E-4	0.49388707623391803
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7272	0.4886417010536841	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3121	0.20971544953087845	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2259	0.15179339970850828	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.719495339022058E-5	0.0	0.0	0.05261364850454271	0.0
2	6.719495339022058E-5	0.0	0.0	0.22517028881062917	0.0
3	6.719495339022058E-5	0.0	0.0	0.3382593953663704	0.0
4	6.719495339022058E-5	0.0	0.0	0.5595323768803667	0.0
5	6.719495339022058E-5	0.0	0.0	0.9840700923997804	0.0
6	6.719495339022058E-5	0.0	0.0	1.4399878511524271	0.0
7	6.719495339022058E-5	0.0	0.0	1.6914313667386325	0.0
8	6.719495339022058E-5	0.0	0.0	2.3241390478609496	0.0
9	6.719495339022058E-5	0.0	0.0	2.572290010731034	0.0
10	6.719495339022058E-5	0.0	0.0	3.0638210947804976	0.0
11	6.719495339022058E-5	0.0	0.0	3.7080863078859325	0.0
12	6.719495339022058E-5	0.0	0.0	4.189067784253131	0.0
13	6.719495339022058E-5	0.0	0.0	4.3918621535848175	0.0
14	6.719495339022058E-5	0.0	0.0	4.477132549437007	0.0
15	6.719495339022058E-5	0.0	0.0	4.615218178653911	0.0
16	6.719495339022058E-5	0.0	0.0	4.891389437087717	0.0
17	6.719495339022058E-5	0.0	0.0	5.211035830364996	0.0
18	6.719495339022058E-5	0.0	0.0	5.668230293232057	0.0
19	6.719495339022058E-5	0.0	0.0	5.8700839332162795	0.0
20	6.719495339022058E-5	0.0	0.0	6.1050646852218815	0.0
21	6.719495339022058E-5	0.0	0.0	6.388761778435392	0.0
22	6.719495339022058E-5	0.0	0.0	6.669569488653124	0.0
23	6.719495339022058E-5	0.0	0.0	6.947487815875077	0.0
24	6.719495339022058E-5	0.0	0.0	7.148938286138958	0.0
25	6.719495339022058E-5	0.0	0.0	7.330095880478993	0.0
26	6.719495339022058E-5	0.0	0.0	7.526977093912339	0.0
27	6.719495339022058E-5	0.0	0.0	7.761689066104379	0.0
28	6.719495339022058E-5	0.0	0.0	7.958704669444506	0.0
29	6.719495339022058E-5	0.0	0.0	8.213171957933271	0.0
30	6.719495339022058E-5	0.0	0.0	8.522806303155408	0.0
31	6.719495339022058E-5	0.0	0.0	8.786076130538293	0.0
32	6.719495339022058E-5	0.0	0.0	8.997538648857317	0.0
33	6.719495339022058E-5	0.0	0.0	9.224993566083214	0.0
34	6.719495339022058E-5	0.0	0.0	9.486180349911	0.0
35	6.719495339022058E-5	0.0	0.0	9.804684428980647	0.0
36	6.719495339022058E-5	0.0	0.0	10.05001320380834	0.0
37	6.719495339022058E-5	0.0	0.0	10.317180338487859	0.0
38	6.719495339022058E-5	0.0	0.0	10.644150981684671	0.0
39	6.719495339022058E-5	0.0	0.0	11.317914779328413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCG	35	1.2122473E-7	45.000004	42
TTTACGA	20	7.033957E-4	45.000004	14
CGCGAGA	20	7.033957E-4	45.000004	20
CGCTAAC	20	7.033957E-4	45.000004	31
CCATACG	35	1.2122473E-7	45.000004	1
TATTACG	20	7.033957E-4	45.000004	1
TCCGTCG	20	7.033957E-4	45.000004	14
GTAACGT	20	7.033957E-4	45.000004	24
CGCCCGA	20	7.033957E-4	45.000004	41
TGCCGAT	20	7.033957E-4	45.000004	38
ACGCAAC	25	3.8913626E-5	45.0	44
TTACGTA	25	3.8913626E-5	45.0	43
TCGAACA	25	3.8913626E-5	45.0	37
AACACGA	25	3.8913626E-5	45.0	41
CGAGTAC	30	2.1659362E-6	44.999996	9
ACGATTC	30	2.1659362E-6	44.999996	10
ACTACGG	60	0.0	44.999996	2
TTTACGG	165	0.0	43.636364	2
CGTAAGG	180	0.0	42.5	2
CGTTTTT	3370	0.0	42.062313	1
>>END_MODULE