FastQCFastQC Report
Thu 26 May 2016
SRR1547850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547850_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2988676
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155960.5218364252264214No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC41050.13735179055876248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA7000.043.71428731
TCGATAG2150.041.8604621
GTTTACG700.041.7857171
TTAACGG2150.040.813952
CACGACC2050.040.60975627
CGTTTTT79950.040.4971851
CACGACG7700.040.32467326
CGACGGT7650.040.2941228
ACGGTCT7700.039.74025730
AGGGTAC11700.039.6153836
TCACGAC7950.039.33962225
CTCACGA7950.039.33962224
TACGGGA9000.038.754
ATTACGA356.2516447E-638.5714333
TAGGGTA14300.037.9195825
GGGCGAT47200.037.754247
TAAGGGA30700.037.671014
CGCTGAA8900.037.6685437
TTAGGGA34850.037.6398854
GGCGATA10400.037.4278878
Produced by FastQC (version 0.11.5)