FastQCFastQC Report
Thu 26 May 2016
SRR1547849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547849_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1378269
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.39302922724083617No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC50770.3683606030462849No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGGT302.165818E-645.00000411
ACGTCGA302.165818E-645.00000416
AATCGGT302.165818E-645.00000426
TAATCCG302.165818E-645.00000442
GTTTGCG700.045.01
CGGGTAC700.045.06
AGGTACG502.1827873E-1145.01
GTACCCG253.8912112E-545.09
GTACCCC207.0337765E-445.044
CGAACGG750.045.02
CAACGAA253.8912112E-545.019
TCCGATC253.8912112E-545.018
CGTGATA207.0337765E-445.033
TTAGACT253.8912112E-545.040
CGTTGCA207.0337765E-445.039
CGTCTTA253.8912112E-545.037
GTTAGCG700.045.01
CGTCTCA253.8912112E-545.035
GTAGGTT207.0337765E-445.024
CGACAGT207.0337765E-445.014