##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547849_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1378269
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.520367939785338	31.0	31.0	33.0	30.0	34.0
2	31.92995199050403	31.0	31.0	34.0	30.0	34.0
3	32.01710696533115	33.0	31.0	34.0	30.0	34.0
4	35.76661304868643	37.0	35.0	37.0	35.0	37.0
5	35.69181270129416	37.0	35.0	37.0	33.0	37.0
6	35.71972670066584	37.0	35.0	37.0	33.0	37.0
7	36.01760904438829	37.0	35.0	37.0	35.0	37.0
8	36.03971866159654	37.0	35.0	37.0	35.0	37.0
9	37.82887883279679	39.0	38.0	39.0	35.0	39.0
10	37.39422057667988	39.0	37.0	39.0	35.0	39.0
11	37.05317829828575	39.0	37.0	39.0	33.0	39.0
12	36.04699227799508	37.0	35.0	39.0	32.0	39.0
13	35.6309160258266	37.0	35.0	39.0	31.0	39.0
14	36.577063693662126	38.0	35.0	41.0	31.0	41.0
15	36.89563430651056	38.0	35.0	41.0	32.0	41.0
16	37.08785077513896	38.0	35.0	41.0	33.0	41.0
17	37.0410543950419	38.0	35.0	41.0	33.0	41.0
18	36.98308748147132	38.0	35.0	41.0	32.0	41.0
19	36.88961226001601	38.0	35.0	41.0	32.0	41.0
20	36.725281494396235	38.0	35.0	41.0	32.0	41.0
21	36.57252902009695	38.0	35.0	40.0	32.0	41.0
22	36.44340691113273	37.0	35.0	40.0	31.0	41.0
23	36.39206279761063	37.0	35.0	40.0	32.0	41.0
24	36.37783045254591	37.0	35.0	40.0	32.0	41.0
25	36.3231154440824	37.0	35.0	40.0	32.0	41.0
26	36.207736660985624	37.0	35.0	40.0	31.0	41.0
27	36.10635006664156	37.0	35.0	40.0	31.0	41.0
28	36.09486972427008	36.0	35.0	40.0	31.0	41.0
29	36.13663370503146	36.0	35.0	40.0	31.0	41.0
30	36.080191892874325	36.0	35.0	40.0	31.0	41.0
31	35.907985306206555	36.0	35.0	40.0	31.0	41.0
32	35.709771459707795	36.0	35.0	40.0	30.0	41.0
33	35.6190924993597	36.0	35.0	40.0	30.0	41.0
34	35.53364256179309	36.0	35.0	40.0	30.0	41.0
35	35.455768794045284	36.0	35.0	40.0	30.0	41.0
36	35.276006352896275	36.0	34.0	40.0	29.0	41.0
37	35.1172623051088	36.0	34.0	40.0	28.0	41.0
38	35.023587558016615	36.0	34.0	40.0	28.0	41.0
39	34.964262419019796	36.0	34.0	40.0	27.0	41.0
40	34.71434821504365	36.0	34.0	40.0	26.0	41.0
41	34.68758711107919	36.0	34.0	40.0	26.0	41.0
42	34.570401714034055	36.0	34.0	40.0	25.0	41.0
43	34.47260803224915	35.0	34.0	40.0	26.0	41.0
44	34.257993178399865	35.0	34.0	40.0	24.0	41.0
45	34.115565974421536	35.0	34.0	40.0	23.0	41.0
46	34.05623357994702	35.0	34.0	40.0	23.0	41.0
47	33.99101263976771	35.0	33.0	40.0	23.0	41.0
48	33.9354364061007	35.0	34.0	40.0	23.0	41.0
49	33.846732386783714	35.0	34.0	39.0	24.0	41.0
50	33.72248233109792	35.0	33.0	39.0	23.0	41.0
51	33.35325324737043	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	8.0
15	27.0
16	90.0
17	234.0
18	558.0
19	1047.0
20	1909.0
21	3109.0
22	4804.0
23	7470.0
24	10810.0
25	15511.0
26	19814.0
27	22790.0
28	25793.0
29	29512.0
30	34471.0
31	41793.0
32	52271.0
33	70287.0
34	139621.0
35	244557.0
36	84852.0
37	104645.0
38	162318.0
39	299919.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.171368578992926	24.37949340803573	30.25048085678485	13.198657156186492
2	29.57020726723158	26.82894268100059	30.234518805835435	13.366331245932397
3	29.26402610811097	25.681416327291696	31.243538090169626	13.811019474427704
4	25.03371983263064	28.85118942673745	30.947587154612055	15.167503586019857
5	24.076432104327967	32.26119139297191	29.729102228955306	13.93327427374482
6	21.125339102889203	40.73007518851545	26.74543213262433	11.399153575971019
7	87.82922637017883	4.573708035223893	5.902693886316822	1.694371708280459
8	88.58858466670874	3.5439380846554625	5.186505682127364	2.6809715665084246
9	84.84301685665136	5.4047504514721	7.022141541310151	2.7300911505663987
10	53.593456719987174	24.341619814419392	11.925248264308348	10.13967520128509
11	46.407196273006214	20.036727228139064	20.80602552912385	12.750050969730871
12	42.69645475592936	20.70096621196588	22.35884286739381	14.243736164710953
13	21.651578900780617	42.44744676111847	21.733565798838978	14.167408539261931
14	14.171761825884497	44.570834866053	26.085691544974164	15.17171176308834
15	12.241006653998603	25.85837742849908	48.00456224438045	13.896053673121866
16	14.789493197626879	20.07503615041766	46.66476573150815	18.470704920447314
17	14.969936928132316	21.220168196484142	26.606417179810325	37.20347769557322
18	19.7986024498846	25.44837038343023	33.33449420976602	21.41853295691915
19	27.049871977095908	26.279122580570263	24.799875786221705	21.871129656112124
20	29.524207538586445	24.78166453718396	24.601293361455564	21.09283456277403
21	20.86820497304953	30.027592581709378	27.959708881212592	21.1444935640285
22	22.58782574374088	25.08944190139951	24.991783171499904	27.33094918335971
23	18.341847636419306	31.700197856876994	23.72076858726417	26.237185919439526
24	19.389611171694348	25.56242649294151	37.64577161642611	17.40219071893803
25	16.68302776888982	26.69145137850449	33.955490546475325	22.67003030613037
26	15.978811102912422	36.308877294635515	25.9257082615948	21.786603340857262
27	16.680851125578535	35.90322353618923	28.558430901369764	18.85749443686247
28	13.83706663938607	31.43863788563771	37.7294998291335	16.99479564584272
29	13.967084799846766	25.99579617621814	37.402640558555696	22.6344784653794
30	16.981663231197974	32.16643485415401	31.524470186879338	19.32743172776867
31	28.666319854832402	28.51584124724564	23.81291315410852	19.004925743813438
32	28.255950035878335	29.52696462011407	24.85559785499057	17.361487489017023
33	26.57608928300644	30.078308370862295	23.446366420488307	19.899235925642962
34	18.91510292983445	30.28153430135917	27.16661261335777	23.63675015544861
35	18.877084226664024	27.467569828531296	29.735704713666202	23.919641231138478
36	28.98759240757791	25.720595906894804	26.92072447395973	18.371087211567552
37	20.907457107429682	30.915735607490262	29.436851587026915	18.739955698053137
38	20.154628740833612	32.155479082820555	23.722727566244327	23.96716461010151
39	19.56954701876049	31.265739851944723	27.846668538579916	21.318044590714873
40	23.11805605436965	26.81559260202471	25.545811448998705	24.52053989460693
41	16.76661087204312	23.471688037676245	28.994412556619935	30.767288533660704
42	22.02059249682029	25.597905778915436	24.866771290655162	27.514730433609113
43	22.439378669911317	26.600104914207606	26.280646230888166	24.679870184992918
44	19.169334868592415	31.498350467143933	26.773946160002147	22.558368504261505
45	17.467344908722463	37.165241328071666	22.108746550927286	23.25866721227859
46	22.92905086017316	32.07617671151277	25.48174558086992	19.51302684744415
47	21.583304855583343	27.46945625273441	26.883286209005647	24.063952682676604
48	22.760796332210912	24.47795024048281	30.27319050199925	22.488062925307034
49	21.52010964477907	23.233635814198824	30.830846518350192	24.415408022671915
50	18.88063941073912	30.11995481288486	28.618143482875986	22.381262293500033
51	17.487877910625574	32.51701953682481	24.51749259397113	25.47760995857848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	903.0
1	1223.0
2	1543.0
3	4873.0
4	8203.0
5	6436.5
6	4670.0
7	4877.0
8	5084.0
9	5746.0
10	6408.0
11	6850.5
12	7293.0
13	7299.0
14	7305.0
15	6932.0
16	6559.0
17	6303.5
18	6048.0
19	6050.0
20	6052.0
21	5962.5
22	5873.0
23	6373.0
24	6873.0
25	7201.0
26	9058.0
27	10587.0
28	11985.5
29	13384.0
30	16113.5
31	18843.0
32	22502.0
33	26161.0
34	29175.0
35	32189.0
36	35422.5
37	38656.0
38	46430.0
39	54204.0
40	75197.5
41	96191.0
42	117757.0
43	139323.0
44	144868.5
45	150414.0
46	144648.0
47	138882.0
48	133441.5
49	128001.0
50	118101.0
51	108201.0
52	95049.0
53	81897.0
54	70988.5
55	60080.0
56	56244.5
57	52409.0
58	47237.5
59	42066.0
60	38626.5
61	35187.0
62	29615.5
63	24044.0
64	20689.0
65	17334.0
66	14241.5
67	11149.0
68	8936.0
69	6723.0
70	5550.5
71	4378.0
72	3707.0
73	3036.0
74	2560.0
75	1624.5
76	1165.0
77	922.0
78	679.0
79	546.5
80	414.0
81	262.5
82	111.0
83	95.0
84	79.0
85	54.0
86	29.0
87	22.5
88	16.0
89	10.0
90	4.0
91	4.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1378269.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.059438327379237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16212794813069	15.278451338188805
2	8.213134358301383	3.130754555530493
3	2.7023358209064674	1.5451500875530373
4	1.3327244705422052	1.0160391941475324
5	0.750364926723396	0.7150767021956504
6	0.46372140935574135	0.5302961761620681
7	0.3001545448775651	0.40045439257435755
8	0.22866671540765784	0.3486607327869307
9	0.1973234434937252	0.33847866016332817
>10	2.3011544381668245	10.900094837881282
>50	1.8988932693836849	27.414620747988383
>100	1.4424655758414993	36.80607263805814
>500	0.005777565724331772	0.7267732401948584
>1k	3.851710482887849E-4	0.07869706196857457
>5k	7.703420965775698E-4	0.7703796346065499
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5417	0.39302922724083617	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5077	0.3683606030462849	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4510955408559577E-4	0.0	0.0	0.07168411971828431	0.0
2	1.4510955408559577E-4	0.0	0.0	0.2815850897030986	0.0
3	1.4510955408559577E-4	0.0	0.0	0.4333696832766318	0.0
4	2.1766433112839365E-4	0.0	0.0	0.8257459175240828	0.0
5	2.1766433112839365E-4	0.0	0.0	1.347197100130671	0.0
6	2.1766433112839365E-4	0.0	0.0	2.0819593272430854	0.0
7	2.1766433112839365E-4	0.0	0.0	2.401853339224781	0.0
8	2.1766433112839365E-4	0.0	0.0	3.216498375861316	0.0
9	2.1766433112839365E-4	0.0	0.0	3.5020014235247254	0.0
10	2.1766433112839365E-4	0.0	0.0	4.014455813778007	0.0
11	2.1766433112839365E-4	0.0	0.0	4.646625586151905	0.0
12	2.1766433112839365E-4	0.0	0.0	5.11532944584838	0.0
13	2.1766433112839365E-4	0.0	0.0	5.2973693814487595	0.0
14	2.1766433112839365E-4	0.0	0.0	5.369706494160429	0.0
15	2.1766433112839365E-4	0.0	0.0	5.484705815773263	0.0
16	2.1766433112839365E-4	0.0	0.0	5.758237325224611	0.0
17	2.1766433112839365E-4	0.0	0.0	6.051140960146387	0.0
18	2.1766433112839365E-4	0.0	0.0	6.483930205206676	0.0
19	2.1766433112839365E-4	0.0	0.0	6.669670434436238	0.0
20	2.1766433112839365E-4	0.0	0.0	6.850331829272805	0.0
21	2.1766433112839365E-4	0.0	0.0	7.084611204343999	0.0
22	2.1766433112839365E-4	0.0	0.0	7.308442691521031	0.0
23	2.1766433112839365E-4	0.0	0.0	7.572179306071601	0.0
24	2.1766433112839365E-4	0.0	0.0	7.751752379252526	0.0
25	2.1766433112839365E-4	0.0	0.0	7.903827191934231	0.0
26	2.1766433112839365E-4	0.0	0.0	8.049952512898425	0.0
27	2.1766433112839365E-4	0.0	0.0	8.192740314118652	0.0
28	2.1766433112839365E-4	0.0	0.0	8.331900376486738	0.0
29	2.1766433112839365E-4	0.0	0.0	8.487385263689454	0.0
30	2.1766433112839365E-4	0.0	0.0	8.70359849927699	0.0
31	2.1766433112839365E-4	0.0	0.0	8.903051581367643	0.0
32	2.1766433112839365E-4	0.0	0.0	9.069782459011993	0.0
33	2.1766433112839365E-4	0.0	0.0	9.231361947486304	0.0
34	2.1766433112839365E-4	0.0	0.0	9.407452391369175	0.0
35	2.1766433112839365E-4	0.0	0.0	9.641949430771497	0.0
36	2.1766433112839365E-4	0.0	0.0	9.812598266376158	0.0
37	2.1766433112839365E-4	0.0	0.0	9.995363749746966	0.0
38	2.1766433112839365E-4	0.0	0.0	10.164634044587812	0.0
39	2.1766433112839365E-4	0.0	0.0	10.359733840055895	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAACGGT	30	2.165818E-6	45.000004	11
ACGTCGA	30	2.165818E-6	45.000004	16
AATCGGT	30	2.165818E-6	45.000004	26
TAATCCG	30	2.165818E-6	45.000004	42
GTTTGCG	70	0.0	45.0	1
CGGGTAC	70	0.0	45.0	6
AGGTACG	50	2.1827873E-11	45.0	1
GTACCCG	25	3.8912112E-5	45.0	9
GTACCCC	20	7.0337765E-4	45.0	44
CGAACGG	75	0.0	45.0	2
CAACGAA	25	3.8912112E-5	45.0	19
TCCGATC	25	3.8912112E-5	45.0	18
CGTGATA	20	7.0337765E-4	45.0	33
TTAGACT	25	3.8912112E-5	45.0	40
CGTTGCA	20	7.0337765E-4	45.0	39
CGTCTTA	25	3.8912112E-5	45.0	37
GTTAGCG	70	0.0	45.0	1
CGTCTCA	25	3.8912112E-5	45.0	35
GTAGGTT	20	7.0337765E-4	45.0	24
CGACAGT	20	7.0337765E-4	45.0	14
>>END_MODULE