FastQCFastQC Report
Thu 26 May 2016
SRR1547848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547848_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1588918
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67460.42456564781820083No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATC6750.072.8888856
CGTAAGG3800.072.631582
CGTTTTT46650.071.082531
ATAGCGG3800.069.473692
GAGCGTA351.1817792E-768.571439
CGAGGGA10650.066.4788744
GTAGGGT11650.065.57944
TACGGGA6800.064.705884
CGAGGGT1800.064.444444
TAGGGAT31150.064.333875
TTCGCGA251.2356718E-464.01
TAGGGTA11350.063.4361275
CGTTTGG4050.063.2098732
ATAGGGA26550.063.126184
CCGATCC13150.062.9657880
TTTGCGG4200.062.857142
TTAGGGA24650.062.799194
AGCGGGA13750.062.5454564
ACGGGAC5350.062.0560725
TAGGGAC22850.061.794315
Produced by FastQC (version 0.11.5)