##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547848_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1588918
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.02208421076481	33.0	31.0	34.0	31.0	34.0
2	32.443085798008454	34.0	31.0	34.0	31.0	34.0
3	32.56203844377117	34.0	31.0	34.0	31.0	34.0
4	36.081316971675065	37.0	35.0	37.0	35.0	37.0
5	36.04274166445342	37.0	35.0	37.0	35.0	37.0
6	36.08740853838902	37.0	35.0	37.0	35.0	37.0
7	36.327982941851	37.0	37.0	37.0	35.0	37.0
8	36.334438278123855	37.0	37.0	37.0	35.0	37.0
9	38.12181874709708	39.0	39.0	39.0	37.0	39.0
10-11	37.68754145903061	39.0	37.5	39.0	35.0	39.0
12-13	36.97620550588513	39.0	35.0	39.0	33.5	39.0
14-15	38.10577512495925	40.0	36.0	41.0	33.0	41.0
16-17	38.27870790059651	40.0	36.0	41.0	34.0	41.0
18-19	38.15291915630637	40.0	36.0	41.0	34.0	41.0
20-21	37.98964672815086	40.0	35.0	41.0	34.0	41.0
22-23	37.8063814495147	40.0	35.0	41.0	34.0	41.0
24-25	37.79372881419935	40.0	35.0	41.0	34.0	41.0
26-27	37.79395632751344	40.0	35.0	41.0	34.0	41.0
28-29	37.72689465409795	40.0	35.0	41.0	34.0	41.0
30-31	37.58605636036599	40.0	35.0	41.0	33.5	41.0
32-33	37.3317915084353	40.0	35.0	41.0	33.0	41.0
34-35	37.04951954726424	40.0	35.0	41.0	32.0	41.0
36-37	36.88868651497434	40.0	35.0	41.0	32.0	41.0
38-39	36.80605575618125	39.5	35.0	41.0	31.0	41.0
40-41	36.68201820358256	39.0	35.0	41.0	31.0	41.0
42-43	36.64574635066127	39.0	35.0	41.0	31.0	41.0
44-45	36.50783426205757	39.0	35.0	41.0	31.0	41.0
46-47	36.37814506475476	39.0	35.0	41.0	31.0	41.0
48-49	36.29301008610891	39.0	35.0	41.0	31.0	41.0
50-51	36.06987270582874	38.0	35.0	40.0	30.0	41.0
52-53	35.88993327534838	38.0	35.0	40.0	30.0	41.0
54-55	35.692722657808645	37.5	35.0	40.0	29.5	41.0
56-57	35.38704388772737	37.0	35.0	40.0	28.5	41.0
58-59	35.12136623790529	36.5	34.5	40.0	28.0	41.0
60-61	34.780778177350875	36.0	34.0	39.5	27.5	41.0
62-63	34.343585383260816	35.5	34.0	39.0	26.5	40.5
64-65	33.98165921715281	35.0	34.0	39.0	26.0	40.0
66-67	33.649865820640215	35.0	34.0	38.0	25.5	40.0
68-69	33.16791804234076	35.0	33.0	37.0	24.0	40.0
70-71	32.866439929562134	35.0	33.0	37.0	24.0	39.0
72-73	32.58802373690776	35.0	33.0	36.0	23.0	39.0
74-75	32.28355679777056	35.0	33.0	36.0	22.0	38.5
76-77	30.35047308923431	33.0	30.0	34.5	19.5	36.0
78-79	31.351711038580973	34.0	31.5	35.0	20.0	37.0
80-81	31.499480464064227	35.0	32.0	35.0	20.0	36.5
82-83	31.300622184404734	35.0	32.0	35.0	19.0	36.0
84-85	31.079047502766034	34.0	32.0	35.0	18.5	36.0
86	30.652583078547792	34.0	31.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	11.0
11	10.0
12	23.0
13	50.0
14	205.0
15	560.0
16	1397.0
17	3052.0
18	5965.0
19	9696.0
20	11865.0
21	12509.0
22	11431.0
23	10371.0
24	10618.0
25	11936.0
26	14467.0
27	17394.0
28	21293.0
29	26657.0
30	33603.0
31	41252.0
32	50270.0
33	65164.0
34	160673.0
35	154071.0
36	132715.0
37	251026.0
38	365170.0
39	165101.0
40	358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.77684436830598	23.719600382146844	30.639655413306414	13.863899836240762
2	30.110490283324882	25.28922197369531	31.175177070182354	13.425110672797464
3	28.82282156788456	25.541280292626805	32.489908226856265	13.14598991263237
4	26.298965711257598	27.110461332806352	31.994665552281486	14.595907403654563
5	24.811349610237908	30.883091512589072	30.167510217644967	14.138048659528057
6	22.42475697298413	40.058706616703944	26.786781948470594	10.72975446184133
7	88.71477319786169	3.614283430611271	6.009938838882812	1.6610045326442269
8	89.57397423907338	2.6798739771341253	5.822138083903638	1.9240136998888553
9	84.71355979351986	5.760649699984518	6.9235794421109205	2.6022110643846945
10-11	38.34310518226869	29.80726506968893	19.863831865458128	11.985797882584249
12-13	29.67802617882106	24.913966611241108	28.22102839794124	17.186978811996592
14-15	17.28283020269139	31.224990842825118	34.20579287288582	17.286386081597666
16-17	23.015504890749554	27.188690668744393	30.9856141097275	18.810190330778557
18-19	25.230660109584008	28.474911858258263	28.907155687077623	17.38727234508011
20-21	26.936537945948125	27.82799364095567	28.959329556339597	16.27613885675661
22-23	22.64833679271051	24.14488979292827	31.607357963091864	21.599415451269355
24-25	20.688481092164604	27.45667177286682	32.88306885566152	18.97177827930705
26-27	18.547055291714234	33.171850277987915	28.428119009288082	19.85297542100977
28-29	17.617806582844427	28.418112199622637	34.16145452439962	19.802626693133316
30-31	22.397096640607003	28.156739366033996	30.373971469893345	19.072192523465652
32-33	25.07580630340899	27.000292022621686	28.60298643479399	19.32091523917534
34-35	19.7556450364336	28.143082273597503	30.5031788928063	21.598093797162598
36-37	22.169551858560354	29.9099764745569	29.737815293174346	18.182656373708397
38-39	20.804629313784602	30.54238796464009	27.745736406787515	20.907246314787802
40-41	21.06933775059506	26.430596166699605	28.986454933483035	23.513611149222303
42-43	20.638981999071067	26.44944547169835	28.56229836907883	24.34927416015175
44-45	20.122876070382485	29.660309720199535	28.60160184477739	21.615212364640595
46-47	20.779234674161913	28.942997687734675	28.579668680196207	21.698098957907206
48-49	21.05769460727363	26.625099596077327	31.099842786097202	21.217363010551836
50-51	19.28554525784213	29.18580442791887	29.342042824110496	22.1866074901285
52-53	21.832183913833187	25.612806954166295	30.503525040310453	22.051484091690067
54-55	21.549098191347824	26.723908974534872	30.217984817341108	21.509008016776196
56-57	22.52177267801107	25.697392816998736	29.473799151372194	22.307035353617994
58-59	22.23100877452455	28.12203021175416	28.892774831677908	20.754186182043377
60-61	23.71220541273999	28.569158383251995	27.684310958778234	20.034325245229773
62-63	22.184027117824833	25.916283911441624	30.86452541918463	21.035163551548916
64-65	20.62164315590861	29.074061719988066	29.922280444931708	20.38201467917161
66-67	19.556389945862527	29.022485741869623	26.88065085800526	24.54047345426259
68-69	18.827717981670546	31.871720252398173	26.411054566692556	22.889507199238725
70-71	19.314306968641553	29.22567432680604	29.670159190090363	21.789859514462044
72-73	21.691270411688958	29.94374159018905	27.660458248946767	20.704529749175222
74-75	20.024979262617705	31.395389818731996	26.3561429853523	22.223487933298006
76-77	22.54024436755075	28.082474992416223	27.457489939694813	21.91979070033822
78-79	18.950033922455408	28.803437307652125	29.545325812911678	22.701202956980786
80-81	17.60459004177686	28.145788517720867	30.053029797635876	24.1965916428664
82-83	19.212791346060655	27.850870844184534	30.578796388485753	22.357541421269065
84-85	17.59096441729529	27.09963635631291	29.568140080230698	25.7412591461611
86	18.850060229665722	28.184777313870192	29.504228663782524	23.46093379268156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	551.0
1	752.5
2	2341.5
3	2572.5
4	1563.5
5	1976.0
6	2417.5
7	2594.0
8	2721.0
9	2840.5
10	2810.0
11	2788.0
12	2805.5
13	2805.5
14	2789.0
15	3001.5
16	3306.0
17	3322.0
18	3244.0
19	3438.0
20	3730.0
21	3826.0
22	4308.5
23	6081.5
24	7075.0
25	8411.0
26	11324.5
27	13460.0
28	15700.0
29	17085.0
30	19718.0
31	23710.0
32	26752.0
33	30914.0
34	35146.5
35	40653.5
36	44407.0
37	44552.0
38	48270.5
39	55168.0
40	61470.0
41	69556.0
42	75419.5
43	76175.0
44	78835.0
45	82034.5
46	87466.0
47	89753.0
48	89134.0
49	85320.5
50	79521.0
51	70061.0
52	58324.0
53	52948.5
54	46388.5
55	41824.0
56	38361.5
57	36002.0
58	33453.0
59	25514.0
60	17376.0
61	12683.5
62	9530.5
63	7312.5
64	6303.0
65	5238.5
66	3544.5
67	2463.0
68	1698.5
69	1300.0
70	870.0
71	526.0
72	418.0
73	264.5
74	145.0
75	67.5
76	50.5
77	45.0
78	33.0
79	13.0
80	11.0
81	9.0
82	8.0
83	5.5
84	4.0
85	2.5
86	1.0
87	3.5
88	4.0
89	3.5
90	3.5
91	1.5
92	1.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1588918.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.612973458691833
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.55785213854043	14.808081902851825
2	8.168286966773081	3.040722170310516
3	2.592771258317243	1.4477754784655363
4	1.2444737622473656	0.9265342842699433
5	0.7329436412375716	0.6821130270535934
6	0.48930789940967445	0.5464484966904314
7	0.3566418288216188	0.46467154258812754
8	0.28050669633921693	0.41768509551577404
9	0.23957691725590954	0.4013314921979508
>10	3.307148080782856	15.494085880478098
>50	1.5826389619866315	21.50094803452422
>100	1.4417096533157143	38.961031527920234
>500	0.004094796648470621	0.5002967672578121
>1k	0.0017061652701960922	0.37981143432800774
>5k	3.4123305403921844E-4	0.4284628655479498
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6746	0.42456564781820083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.888077295367036E-4	0.0	0.0	0.07231336041255748	0.0
2	1.888077295367036E-4	0.0	0.0	0.2401004960608414	0.0
3	1.888077295367036E-4	0.0	0.0	0.3441335550355651	0.0
4	1.888077295367036E-4	0.0	0.0	0.5533954552720782	0.0
5	1.888077295367036E-4	0.0	0.0	0.9726744866632513	0.0
6	1.888077295367036E-4	0.0	0.0	1.292829460047655	0.0
7	1.888077295367036E-4	0.0	0.0	1.4886860114870624	0.0
8	1.888077295367036E-4	0.0	0.0	1.8588120972888469	0.0
9	1.888077295367036E-4	0.0	0.0	1.9685093881496716	0.0
10	1.888077295367036E-4	0.0	0.0	2.201623998217655	0.0
11	1.888077295367036E-4	0.0	0.0	2.583078547791642	0.0
12	1.888077295367036E-4	0.0	0.0	2.883534581394383	0.0
13	1.888077295367036E-4	0.0	0.0	2.990399756312157	0.0
14	1.888077295367036E-4	0.0	0.0	3.034958380482819	0.0
15	1.888077295367036E-4	0.0	0.0	3.1191036919463433	0.0
16	1.888077295367036E-4	0.0	0.0	3.268702349649258	0.0
17	1.888077295367036E-4	0.0	0.0	3.4634260547114453	0.0
18	1.888077295367036E-4	0.0	0.0	3.701134986198155	0.0
19	1.888077295367036E-4	0.0	0.0	3.859607607189295	0.0
20	1.888077295367036E-4	0.0	0.0	4.035135859748584	0.0
21	2.5174363938227143E-4	0.0	0.0	4.241691515861738	0.0
22	2.5174363938227143E-4	0.0	0.0	4.459260956197865	0.0
23	2.5174363938227143E-4	0.0	0.0	4.681361782042875	0.0
24	2.5174363938227143E-4	0.0	0.0	4.8428553267066015	0.0
25	2.5174363938227143E-4	0.0	0.0	4.979677994710866	0.0
26	2.5174363938227143E-4	0.0	0.0	5.126507472380576	0.0
27	2.5174363938227143E-4	0.0	0.0	5.296686172602992	0.0
28	2.5174363938227143E-4	0.0	0.0	5.465920834177724	0.0
29	2.5174363938227143E-4	0.0	0.0	5.660518667420219	0.0
30	2.5174363938227143E-4	0.0	0.0	5.866256156705381	0.0
31	2.5174363938227143E-4	0.0	0.0	6.054119847594401	0.0
32	2.5174363938227143E-4	0.0	0.0	6.2252425864645	0.0
33	2.5174363938227143E-4	0.0	0.0	6.401714877671472	0.0
34	2.5174363938227143E-4	0.0	0.0	6.579886438444275	0.0
35	2.5174363938227143E-4	0.0	0.0	6.797330006960712	0.0
36	2.5174363938227143E-4	0.0	0.0	6.982361581906681	0.0
37	2.5174363938227143E-4	0.0	0.0	7.194896149455164	0.0
38	2.5174363938227143E-4	0.0	0.0	7.414227795266968	0.0
39	2.5174363938227143E-4	0.0	0.0	7.7162572266158485	0.0
40	2.5174363938227143E-4	0.0	0.0	7.94427402798634	0.0
41	2.5174363938227143E-4	0.0	0.0	8.175815240308184	0.0
42	2.5174363938227143E-4	0.0	0.0	8.411069671310917	0.0
43	2.5174363938227143E-4	0.0	0.0	8.60755558184878	0.0
44	2.5174363938227143E-4	0.0	0.0	8.81203435293703	0.0
45	2.5174363938227143E-4	0.0	0.0	9.006569250269681	0.0
46	2.5174363938227143E-4	0.0	0.0	9.232005679336504	0.0
47	2.5174363938227143E-4	0.0	0.0	9.473239021774566	0.0
48	2.5174363938227143E-4	0.0	0.0	9.69697618127556	0.0
49	2.5174363938227143E-4	0.0	0.0	9.933300522745666	0.0
50	2.5174363938227143E-4	0.0	0.0	10.166163389174269	0.0
51	2.5174363938227143E-4	0.0	0.0	10.398082216955185	0.0
52	2.5174363938227143E-4	0.0	0.0	10.668014334282827	0.0
53	2.5174363938227143E-4	0.0	0.0	10.911387497655637	0.0
54	2.5174363938227143E-4	0.0	0.0	11.174711344449493	0.0
55	2.5174363938227143E-4	0.0	0.0	11.419594969658597	0.0
56	2.5174363938227143E-4	0.0	0.0	11.685562124665967	0.0
57	2.5174363938227143E-4	0.0	0.0	11.925914364366191	0.0
58	2.5174363938227143E-4	0.0	0.0	12.15896603852433	0.0
59	2.5174363938227143E-4	0.0	0.0	12.418513730727451	0.0
60	2.5174363938227143E-4	0.0	0.0	12.679760692496403	0.0
61	2.5174363938227143E-4	0.0	0.0	12.959888427219026	0.0
62	2.5174363938227143E-4	0.0	0.0	13.289483787080265	0.0
63	3.146795492278393E-4	0.0	0.0	13.606743708611772	0.0
64	3.146795492278393E-4	0.0	0.0	13.901535510328412	0.0
65	3.146795492278393E-4	0.0	0.0	14.179963975485204	0.0
66	3.146795492278393E-4	0.0	0.0	14.435987256736974	0.0
67	3.146795492278393E-4	0.0	0.0	14.70585643815477	0.0
68	3.146795492278393E-4	0.0	0.0	15.00901871588087	0.0
69	3.146795492278393E-4	0.0	0.0	15.404507973350418	0.0
70	3.146795492278393E-4	0.0	0.0	15.697411697771692	0.0
71	3.146795492278393E-4	0.0	0.0	15.974077957452808	0.0
72	3.776154590734072E-4	0.0	0.0	16.255779089921568	0.0
73	3.776154590734072E-4	0.0	0.0	16.544403172473345	0.0
74	4.4055136891897503E-4	0.0	0.0	16.88029212331914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATC	675	0.0	72.888885	6
CGTAAGG	380	0.0	72.63158	2
CGTTTTT	4665	0.0	71.08253	1
ATAGCGG	380	0.0	69.47369	2
GAGCGTA	35	1.1817792E-7	68.57143	9
CGAGGGA	1065	0.0	66.478874	4
GTAGGGT	1165	0.0	65.5794	4
TACGGGA	680	0.0	64.70588	4
CGAGGGT	180	0.0	64.44444	4
TAGGGAT	3115	0.0	64.33387	5
TTCGCGA	25	1.2356718E-4	64.0	1
TAGGGTA	1135	0.0	63.436127	5
CGTTTGG	405	0.0	63.209873	2
ATAGGGA	2655	0.0	63.12618	4
CCGATCC	1315	0.0	62.96578	80
TTTGCGG	420	0.0	62.85714	2
TTAGGGA	2465	0.0	62.79919	4
AGCGGGA	1375	0.0	62.545456	4
ACGGGAC	535	0.0	62.056072	5
TAGGGAC	2285	0.0	61.79431	5
>>END_MODULE