FastQCFastQC Report
Thu 26 May 2016
SRR1547847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547847_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1776781
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136350.7673990210386086No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80550.075.282431
TATAGCG1100.069.090911
ACGGGAT11050.068.41635
CGAGGGA13100.067.480914
TAACGGG4950.066.262623
GTACGGG8500.065.8823553
TACGGGA8450.065.798824
CGAAGGG9700.065.154643
CGTTAGG3350.064.4776152
CGCGAGG2300.064.3478242
CGAGGGT2300.064.3478244
TAGGGTA8150.064.294485
TCGCGCG750.064.01
AGGGATT38800.063.711346
CGGGATT8750.063.542866
CGGGATC7950.063.3962256
TAGGGAT39300.063.3078885
GCGAGGG14850.062.491583
TAGGGAC20250.062.4197545
CGATAGG4850.061.855672
Produced by FastQC (version 0.11.5)