##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547847_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1776781
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06288563418902	33.0	31.0	34.0	31.0	34.0
2	32.49073633723008	34.0	31.0	34.0	31.0	34.0
3	32.585867363507376	34.0	31.0	34.0	31.0	34.0
4	36.122248605765144	37.0	35.0	37.0	35.0	37.0
5	36.088565219911736	37.0	35.0	37.0	35.0	37.0
6	36.14605739255429	37.0	36.0	37.0	35.0	37.0
7	36.3749775577294	37.0	37.0	37.0	35.0	37.0
8	36.38720810274311	37.0	37.0	37.0	35.0	37.0
9	38.1996745800411	39.0	39.0	39.0	37.0	39.0
10-11	37.73457167765751	39.0	37.5	39.0	35.0	39.0
12-13	37.22092846557904	39.0	37.0	39.0	34.5	39.0
14-15	38.479836288208844	40.0	37.5	41.0	34.0	41.0
16-17	38.58969056963126	40.0	37.5	41.0	35.0	41.0
18-19	38.437438828983424	40.0	37.0	41.0	34.0	41.0
20-21	38.23342550376214	40.0	36.0	41.0	34.0	41.0
22-23	38.03978599501008	40.0	35.0	41.0	34.0	41.0
24-25	38.02428380312486	40.0	35.0	41.0	34.0	41.0
26-27	38.004941520648856	40.0	35.0	41.0	34.0	41.0
28-29	37.93375942223605	40.0	36.0	41.0	34.0	41.0
30-31	37.765024502175564	40.0	35.5	41.0	33.5	41.0
32-33	37.425044504640695	40.0	35.0	41.0	33.0	41.0
34-35	37.112162106641165	40.0	35.0	41.0	32.5	41.0
36-37	36.90804381631726	40.0	35.0	41.0	31.5	41.0
38-39	36.813133132333135	40.0	35.0	41.0	31.0	41.0
40-41	36.72050410264405	40.0	35.0	41.0	31.0	41.0
42-43	36.666976402831864	40.0	35.0	41.0	31.0	41.0
44-45	36.53646847867013	39.5	35.0	41.0	31.0	41.0
46-47	36.41268479345513	39.0	35.0	41.0	30.0	41.0
48-49	36.30493319097852	39.0	35.0	41.0	30.0	41.0
50-51	36.081454045265005	39.0	35.0	40.5	30.0	41.0
52-53	35.91077347180097	38.5	35.0	40.0	29.0	41.0
54-55	35.72835397271808	38.0	35.0	40.0	29.0	41.0
56-57	35.464923082811	38.0	35.0	40.0	28.5	41.0
58-59	35.205367178059646	37.0	35.0	40.0	28.0	41.0
60-61	34.85097685083305	36.5	34.0	40.0	27.0	41.0
62-63	34.50047923745245	36.0	34.0	39.0	26.5	41.0
64-65	34.1532231603107	35.5	34.0	39.0	26.0	40.0
66-67	33.836605918230774	35.0	34.0	38.5	26.0	40.0
68-69	33.371573649200435	35.0	33.5	37.5	24.0	40.0
70-71	33.0582623857414	35.0	33.0	37.0	24.0	39.5
72-73	32.78807517640047	35.0	33.0	37.0	24.0	39.0
74-75	32.464475925845676	35.0	33.0	36.0	23.0	38.5
76-77	30.49464171442626	33.0	30.0	34.5	19.5	36.0
78-79	31.52634680357343	34.5	31.5	35.0	20.0	37.0
80-81	31.646054297068687	35.0	32.0	35.0	20.0	37.0
82-83	31.43744417573128	35.0	32.0	35.0	19.5	36.0
84-85	31.201444353580996	34.5	32.0	35.0	19.0	36.0
86	30.75511388291523	34.0	31.0	35.0	18.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	10.0
11	12.0
12	27.0
13	62.0
14	220.0
15	693.0
16	1775.0
17	4317.0
18	9973.0
19	15033.0
20	16898.0
21	16097.0
22	13387.0
23	11442.0
24	10790.0
25	11661.0
26	13423.0
27	16384.0
28	20162.0
29	25496.0
30	32183.0
31	40814.0
32	50376.0
33	65586.0
34	154725.0
35	154472.0
36	149741.0
37	284618.0
38	447196.0
39	208699.0
40	506.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.486547863805388	23.429505380798197	30.818598352863972	14.265348402532446
2	31.78067527736958	24.839921183308466	30.95986505934046	12.419538479981494
3	28.71366814480794	24.800017559845585	33.636165627615334	12.850148667731137
4	25.71014660782617	27.311300604857884	33.373668448728345	13.604884338587592
5	23.74423184399203	31.084416143576505	31.67081367934484	13.50053833308663
6	21.889079183084466	40.30918835804751	27.380470637630637	10.421261821237394
7	88.30142825705587	3.1840727698011175	7.161884329019727	1.352614644123277
8	89.5738979649152	2.3464343664188214	6.8422050888657635	1.2374625798002117
9	85.38142854971997	5.189891157098145	7.738882844875087	1.6897974483067975
10-11	35.414437682528124	30.94779266550014	21.683961050911734	11.953808601060006
12-13	26.635584239138083	25.148456675302132	30.74422227612745	17.47173680943234
14-15	17.422040195161927	31.70582080740395	33.50657734408461	17.365561653349513
16-17	21.968239192112026	27.713038354192214	31.948760145454052	18.369962308241703
18-19	23.379471077189592	28.710094265978757	30.5045754091247	17.40585924770695
20-21	25.716590846029984	28.08258305328569	30.42665359433718	15.774172506347153
22-23	22.341132643809225	24.384884800096355	32.401460844077015	20.872521712017406
24-25	19.64291040933013	28.499713808285883	33.732941763785185	18.124434018598805
26-27	19.060227456281893	32.7748608297815	29.852272170852796	18.312639543083815
28-29	17.89652185609819	28.593450740412013	34.915473544573025	18.594553858916772
30-31	21.692543988257416	28.473374039907	31.46350056647386	18.37058140536172
32-33	23.17888361030425	28.39826630293773	29.93610354905866	18.486746537699357
34-35	20.062489412032207	28.49101830782747	30.757448441873255	20.689043838267068
36-37	21.914152616445133	29.137468264237405	30.138660870416782	18.80971824890068
38-39	19.72167644746314	31.101188047373313	29.067510289675543	20.10962521548801
40-41	20.872409148904676	28.312071099364527	29.655624412913017	21.159895338817783
42-43	20.937808317400965	27.37304147219044	29.42019866263766	22.26895154777094
44-45	19.730006117805175	30.571691165090126	29.071478139399286	20.626824577705413
46-47	21.157615935784996	29.91007332923979	28.55464460729825	20.37766612767696
48-49	21.045277949280187	27.94272338571833	30.828926018456976	20.18307264654451
50-51	19.316927634863273	30.309897505657702	29.766048826501407	20.607126032977614
52-53	21.241278469321767	27.628616019644515	30.73589260578541	20.39421290524831
54-55	21.20598993348083	28.206768307405362	30.2818130090315	20.30542875008231
56-57	21.438657887494294	27.290729696006427	30.011295708362486	21.259316708136794
58-59	22.537358290076266	28.201787389667043	29.20691970479198	20.05393461546471
60-61	22.2141333118713	29.62483840158129	28.328477173044963	19.832551113502454
62-63	21.801279955154858	28.18594413155026	29.924959800898364	20.08781611239652
64-65	20.249119052938994	30.347071473636877	29.547197994575587	19.856611478848546
66-67	19.149602567789728	29.95768752592469	27.545403738558665	23.347306167726916
68-69	19.07837825820965	30.933947402634317	27.50164482848477	22.486029510671266
70-71	19.638013913926365	30.119750267478096	29.13074261825177	21.111493200343766
72-73	21.495108288528524	29.91755877623635	28.094571024791463	20.49276191044366
74-75	20.60380542115207	30.959639933114996	27.05398132915649	21.38257331657644
76-77	22.63714548951165	29.404270982186326	27.644825107877676	20.313758420424353
78-79	19.56510115765533	29.485935520472133	29.6984265365287	21.250536785343833
80-81	18.189608060869627	29.332568279377142	29.828211805506697	22.64961185424653
82-83	19.30538991580842	29.158883396434337	29.7613774573231	21.77434923043414
84-85	18.383413600212968	28.554982296636446	29.180551795634912	23.88105230751567
86	19.08518832652983	28.814412130701534	30.029193243286596	22.07120629948204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	710.0
1	795.5
2	4148.0
3	4767.5
4	2296.0
5	2779.0
6	3317.0
7	3548.0
8	3767.0
9	4007.0
10	4156.5
11	4365.0
12	4272.5
13	4238.5
14	4377.0
15	4426.0
16	4522.5
17	4731.5
18	5112.5
19	5388.0
20	6130.5
21	6817.0
22	6993.5
23	7784.0
24	9574.0
25	11937.5
26	13920.0
27	16300.5
28	20639.5
29	23393.0
30	25577.5
31	30870.5
32	35534.0
33	38738.5
34	43639.0
35	48761.0
36	50632.0
37	52301.5
38	56752.5
39	64194.5
40	72114.5
41	78579.0
42	86099.0
43	90414.0
44	91636.0
45	93416.5
46	95362.0
47	97963.0
48	99037.0
49	90314.0
50	81731.0
51	74067.5
52	61746.0
53	54143.0
54	47008.5
55	40371.5
56	35607.0
57	33290.0
58	29334.5
59	21444.5
60	14942.5
61	10951.0
62	8191.0
63	5903.0
64	4951.0
65	3987.5
66	2541.0
67	1760.5
68	1205.0
69	759.5
70	427.0
71	282.0
72	245.0
73	183.5
74	136.5
75	78.0
76	29.5
77	35.0
78	32.5
79	12.0
80	8.5
81	6.0
82	7.5
83	4.0
84	1.5
85	1.5
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1776781.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.35603618431716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97626484563222	16.076437048999047
2	7.456391039370187	3.035651316036755
3	2.558355966420165	1.5623395987443771
4	1.2739532594355565	1.0373055458479588
5	0.8230723214893734	0.8377244979273805
6	0.6190721745609541	0.7561113351640013
7	0.496619984348802	0.7076450059881463
8	0.40431995478316757	0.6584281303686108
9	0.32475589859456394	0.594966854057524
>10	4.424765875872267	21.832334825146603
>50	1.6596294743558684	24.350601729929984
>100	0.9800150714115407	27.435564622719
>500	0.002227306980230288	0.28410639531878823
>1k	2.78413372528786E-4	0.058034101098431906
>5k	0.0	0.0
>10k+	2.78413372528786E-4	0.7727489926534366
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13635	0.7673990210386086	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.628155636513448E-5	0.0	0.0	0.026564894604343473	0.0
2	5.628155636513448E-5	0.0	0.0	0.09427160691160025	0.0
3	5.628155636513448E-5	0.0	0.0	0.1425049007165205	0.0
4	5.628155636513448E-5	0.0	0.0	0.22794030327879464	0.0
5	5.628155636513448E-5	0.0	0.0	0.45452984920482603	0.0
6	5.628155636513448E-5	0.0	0.0	0.6479132768754281	0.0
7	5.628155636513448E-5	0.0	0.0	0.773421147569678	0.0
8	5.628155636513448E-5	0.0	0.0	0.9973091787901829	0.0
9	5.628155636513448E-5	0.0	0.0	1.074358629454052	0.0
10	5.628155636513448E-5	0.0	0.0	1.2221539964688952	0.0
11	5.628155636513448E-5	0.0	0.0	1.475252155442905	0.0
12	5.628155636513448E-5	0.0	0.0	1.6656526606261548	0.0
13	5.628155636513448E-5	0.0	0.0	1.7388186839008297	0.0
14	5.628155636513448E-5	0.0	0.0	1.771180578810782	0.0
15	5.628155636513448E-5	0.0	0.0	1.8198078435102583	0.0
16	5.628155636513448E-5	0.0	0.0	1.922690528545724	0.0
17	5.628155636513448E-5	0.0	0.0	2.0735813811606496	0.0
18	5.628155636513448E-5	0.0	0.0	2.2540200508672705	0.0
19	5.628155636513448E-5	0.0	0.0	2.369678649197622	0.0
20	5.628155636513448E-5	0.0	0.0	2.4941734518772996	0.0
21	5.628155636513448E-5	0.0	0.0	2.651705528143311	0.0
22	5.628155636513448E-5	0.0	0.0	2.840811557530163	0.0
23	5.628155636513448E-5	0.0	0.0	3.0507980443284795	0.0
24	5.628155636513448E-5	0.0	0.0	3.2015200522743097	0.0
25	5.628155636513448E-5	0.0	0.0	3.3377777002343003	0.0
26	5.628155636513448E-5	0.0	0.0	3.4639609496049317	0.0
27	1.1256311273026895E-4	0.0	0.0	3.6004437237903826	0.0
28	1.1256311273026895E-4	0.0	0.0	3.7531355862089923	0.0
29	1.1256311273026895E-4	0.0	0.0	3.9134817402932605	0.0
30	1.1256311273026895E-4	0.0	0.0	4.098929468516379	0.0
31	1.1256311273026895E-4	0.0	0.0	4.268787205626355	0.0
32	1.1256311273026895E-4	0.0	0.0	4.433579602663468	0.0
33	1.1256311273026895E-4	0.0	0.0	4.60174889308249	0.0
34	1.1256311273026895E-4	0.0	0.0	4.778585543181743	0.0
35	1.1256311273026895E-4	0.0	0.0	4.974276514663315	0.0
36	1.1256311273026895E-4	0.0	0.0	5.155615689271778	0.0
37	1.1256311273026895E-4	0.0	0.0	5.3482674567096335	0.0
38	1.1256311273026895E-4	0.0	0.0	5.549980554722276	0.0
39	1.1256311273026895E-4	0.0	0.0	5.791991247092354	0.0
40	1.1256311273026895E-4	0.0	0.0	6.011264190690918	0.0
41	1.1256311273026895E-4	0.0	0.0	6.221081832820139	0.0
42	1.1256311273026895E-4	0.0	0.0	6.443281417349691	0.0
43	1.1256311273026895E-4	0.0	0.0	6.649778447653369	0.0
44	1.1256311273026895E-4	0.0	0.0	6.863085546277229	0.0
45	1.1256311273026895E-4	0.0	0.0	7.072284091286433	0.0
46	1.1256311273026895E-4	0.0	0.0	7.292570102899569	0.0
47	1.1256311273026895E-4	0.0	0.0	7.523887299560272	0.0
48	1.1256311273026895E-4	0.0	0.0	7.750251719260843	0.0
49	1.1256311273026895E-4	0.0	0.0	7.985733751092566	0.0
50	1.1256311273026895E-4	0.0	0.0	8.22791328813174	0.0
51	1.1256311273026895E-4	0.0	0.0	8.45889279545425	0.0
52	1.1256311273026895E-4	0.0	0.0	8.71570553714836	0.0
53	1.1256311273026895E-4	0.0	0.0	8.959798647103948	0.0
54	1.1256311273026895E-4	0.0	0.0	9.202991252157695	0.0
55	1.1256311273026895E-4	0.0	0.0	9.448716527247871	0.0
56	1.1256311273026895E-4	0.0	0.0	9.698831763734528	0.0
57	1.6884466909540343E-4	0.0	0.0	9.954856563639526	0.0
58	2.251262254605379E-4	0.0	0.0	10.209305479966298	0.0
59	2.251262254605379E-4	0.0	0.0	10.474729299784272	0.0
60	2.251262254605379E-4	0.0	0.0	10.741841566293202	0.0
61	2.8140778182567236E-4	0.0	0.0	11.01762119248236	0.0
62	2.8140778182567236E-4	0.0	0.0	11.30482597461364	0.0
63	2.8140778182567236E-4	0.0	0.0	11.593831766548607	0.0
64	2.8140778182567236E-4	0.0	0.0	11.87788478152344	0.0
65	2.8140778182567236E-4	0.0	0.0	12.152426213472566	0.0
66	2.8140778182567236E-4	0.0	0.0	12.425448043399834	0.0
67	2.8140778182567236E-4	0.0	0.0	12.701621640483548	0.0
68	2.8140778182567236E-4	0.0	0.0	12.99158421887672	0.0
69	2.8140778182567236E-4	0.0	0.0	13.331187129983944	0.0
70	2.8140778182567236E-4	0.0	0.0	13.615859242078793	5.628155636513448E-5
71	2.8140778182567236E-4	0.0	0.0	13.91133741299575	5.628155636513448E-5
72	3.3768933819080686E-4	0.0	0.0	14.203269845861701	5.628155636513448E-5
73	3.3768933819080686E-4	0.0	0.0	14.493119861142144	5.628155636513448E-5
74	3.3768933819080686E-4	0.0	0.0	14.800473440452144	5.628155636513448E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8055	0.0	75.28243	1
TATAGCG	110	0.0	69.09091	1
ACGGGAT	1105	0.0	68.4163	5
CGAGGGA	1310	0.0	67.48091	4
TAACGGG	495	0.0	66.26262	3
GTACGGG	850	0.0	65.882355	3
TACGGGA	845	0.0	65.79882	4
CGAAGGG	970	0.0	65.15464	3
CGTTAGG	335	0.0	64.477615	2
CGCGAGG	230	0.0	64.347824	2
CGAGGGT	230	0.0	64.347824	4
TAGGGTA	815	0.0	64.29448	5
TCGCGCG	75	0.0	64.0	1
AGGGATT	3880	0.0	63.71134	6
CGGGATT	875	0.0	63.54286	6
CGGGATC	795	0.0	63.396225	6
TAGGGAT	3930	0.0	63.307888	5
GCGAGGG	1485	0.0	62.49158	3
TAGGGAC	2025	0.0	62.419754	5
CGATAGG	485	0.0	61.85567	2
>>END_MODULE