FastQCFastQC Report
Thu 26 May 2016
SRR1547846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547846_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1176871
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188111.598390987627361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT90550.076.996131
TAGGGCG2200.067.272735
TACGGGA4900.064.489794
TTAGCGG4050.064.1975252
TAGGGAC12300.062.7642335
TAGCGGG8750.062.628573
CCGATCC6800.061.76470680
AGGGATC14000.061.4285666
AGCGGGA8900.060.224724
TTGCGGG7950.059.874213
AGGGATT21100.059.7156376
CGAGGGA7350.058.7755134
ATAGGGA15600.058.7179534
TATAGCG750.058.666661
GTAGGGA20200.058.6138574
CGGGACT4800.058.3333326
CGAACGG557.003109E-1058.1818162
ATAGCGG2000.058.02
TAGGGAT19200.057.9166645
GCTACGG2150.057.6744162
Produced by FastQC (version 0.11.5)