##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547846_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1176871
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0833345370903	33.0	31.0	34.0	31.0	34.0
2	32.523900240553125	34.0	31.0	34.0	31.0	34.0
3	32.6122412736825	34.0	31.0	34.0	31.0	34.0
4	36.18086094397772	37.0	35.0	37.0	35.0	37.0
5	36.1653843114496	37.0	35.0	37.0	35.0	37.0
6	36.21567954346738	37.0	37.0	37.0	35.0	37.0
7	36.3578497558356	37.0	37.0	37.0	35.0	37.0
8	36.35236997088041	37.0	37.0	37.0	35.0	37.0
9	38.16582106280128	39.0	39.0	39.0	37.0	39.0
10-11	37.82978635721332	39.0	38.0	39.0	35.0	39.0
12-13	37.37187423260494	39.0	37.0	39.0	35.0	39.0
14-15	38.578427457214936	40.0	38.0	41.0	34.0	41.0
16-17	38.72756104959677	40.0	38.0	41.0	35.0	41.0
18-19	38.49351585687811	40.0	37.5	41.0	35.0	41.0
20-21	38.21070448672794	40.0	35.0	41.0	34.0	41.0
22-23	38.04984658471489	40.0	35.0	41.0	34.0	41.0
24-25	37.98161820624351	40.0	35.0	41.0	34.0	41.0
26-27	37.96954763946091	40.0	35.0	41.0	34.0	41.0
28-29	37.87512310185228	40.0	35.0	41.0	34.0	41.0
30-31	37.63854874493467	40.0	35.0	41.0	33.5	41.0
32-33	37.150432800196455	40.0	35.0	41.0	32.5	41.0
34-35	36.65803473787696	40.0	35.0	41.0	30.5	41.0
36-37	36.41094308552084	40.0	35.0	41.0	30.0	41.0
38-39	36.28633512084162	40.0	35.0	41.0	30.0	41.0
40-41	36.13544050282486	40.0	35.0	41.0	29.0	41.0
42-43	36.07669532174724	40.0	35.0	41.0	29.0	41.0
44-45	35.94826280875304	39.0	35.0	41.0	28.0	41.0
46-47	35.8404642479932	39.0	35.0	41.0	28.0	41.0
48-49	35.73090806044163	39.0	35.0	41.0	27.0	41.0
50-51	35.52213114266559	38.5	35.0	40.5	26.5	41.0
52-53	35.33325062814871	38.0	35.0	40.0	26.0	41.0
54-55	35.129292845180146	38.0	35.0	40.0	26.0	41.0
56-57	34.842295799624594	37.0	34.5	40.0	25.0	41.0
58-59	34.59601519622797	37.0	34.0	40.0	25.0	41.0
60-61	34.24854593239191	36.0	34.0	40.0	24.0	41.0
62-63	33.905845245570674	36.0	34.0	39.0	23.0	41.0
64-65	33.56088050432035	35.0	34.0	39.0	22.0	40.0
66-67	33.230210023018664	35.0	33.0	38.5	21.5	40.0
68-69	32.73844457039047	35.0	33.0	37.5	19.5	40.0
70-71	32.45295278751877	35.0	33.0	37.0	19.0	39.0
72-73	32.16836679636086	35.0	33.0	36.5	19.0	39.0
74-75	31.865700233925384	35.0	32.5	36.0	18.0	38.5
76-77	29.97024270289607	33.0	29.5	34.5	14.0	36.0
78-79	31.051669214382883	34.0	31.5	35.0	17.0	37.0
80-81	31.115664758499445	35.0	32.0	35.0	15.5	36.5
82-83	30.93200571685427	34.5	32.0	35.0	15.0	36.0
84-85	30.721578660702832	34.0	31.5	35.0	13.0	36.0
86	30.30629440270004	34.0	31.0	35.0	11.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	12.0
11	12.0
12	17.0
13	52.0
14	154.0
15	495.0
16	1308.0
17	3564.0
18	8809.0
19	14197.0
20	16667.0
21	16394.0
22	13473.0
23	10514.0
24	8572.0
25	8481.0
26	8840.0
27	10694.0
28	12795.0
29	15916.0
30	19914.0
31	25245.0
32	31777.0
33	42014.0
34	96223.0
35	96171.0
36	101970.0
37	192689.0
38	291385.0
39	128216.0
40	297.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.463746663822967	23.499092084009206	28.72931697696689	17.307844275200935
2	34.166106565630386	24.53225544685866	28.71317247174924	12.58846551576171
3	28.13536912711759	23.69197643582007	35.362669315498465	12.809985121563875
4	25.63908873614865	26.04949905299731	34.51431805185105	13.797094159002985
5	23.37070078198885	30.390161708462525	32.70834271555676	13.530794793991866
6	21.369376932561003	38.95873039610969	29.623807537104746	10.048085134224568
7	84.24806117237999	3.5617327642536862	10.51151740505119	1.6786886583151424
8	85.39109214178954	2.9382149785320566	10.19313076794313	1.4775621117352709
9	80.64979084368635	5.406879768470801	11.565668624683589	2.377660763159259
10-11	33.59607807482723	28.10477953828415	25.28034083599647	13.018801550892153
12-13	27.38949298606219	22.97176156095273	31.67751605740986	17.961229395575216
14-15	17.99415568911121	28.955637448794302	35.35277018466765	17.697436677426836
16-17	23.75196601836565	27.017277169715285	32.40448613314458	16.82627067877448
18-19	23.74682526802003	28.321923133461524	31.126563574087555	16.804688024430884
20-21	25.49281102176874	27.26093174188165	32.04004517062617	15.206212065723431
22-23	22.34798886199082	23.590181081868785	34.88334745269447	19.178482603445918
24-25	20.900931368008898	27.972734479819795	33.53842519698421	17.5879089551871
26-27	19.603720373770788	30.810853526002425	31.51951233397713	18.065913766249658
28-29	18.220178762158298	28.833321579000586	34.35818369218037	18.588315966660748
30-31	21.60160289445487	27.61585594342965	32.671975093277005	18.11056606883847
32-33	23.60305420050286	27.968698353515382	30.480188567820942	17.94805887816082
34-35	20.36119506725886	29.173503298152475	30.709313085291424	19.755988549297246
36-37	22.234977325467277	30.240060295478433	29.147544633184097	18.377417745870193
38-39	20.97579088957073	29.547970848121842	28.979216923520077	20.497021338787345
40-41	21.120666581129115	28.226458124977167	29.503573458773307	21.149301835120415
42-43	20.92183425371175	28.235337602846872	29.40708879732783	21.43573934611355
44-45	20.70800453065799	29.089891755341068	29.432155265955235	20.769948448045707
46-47	20.526633760199715	29.26093853956806	29.493249472542022	20.719178227690204
48-49	20.589469873928408	28.611292146717865	30.512817462576614	20.286420516777113
50-51	19.291281712269228	29.69093469037813	30.29176519771496	20.726018399637685
52-53	21.285722904209553	27.831470059165365	29.762607796436484	21.120199240188605
54-55	21.072190579936116	27.66679610594534	30.833625775467322	20.42738753865122
56-57	21.27008822547246	28.20236882377083	29.47243155791926	21.055111392837446
58-59	22.018216100150315	28.668052828219913	29.016816626461186	20.296914445168586
60-61	22.44001254173142	29.47111450617782	27.890397503209783	20.198475448880973
62-63	21.977939808186285	28.13541161265763	29.40368995412411	20.482958625031973
64-65	21.260061638021497	30.322652185328725	28.933927337830568	19.48335883881921
66-67	20.764467813379717	30.184234295857404	27.376195012027654	21.675102878735224
68-69	19.468913755203417	31.31417971893266	27.872213692069902	21.344692833794017
70-71	20.325932069020308	30.401632804275064	28.40761646773521	20.86481865896942
72-73	21.580020240111278	30.70064603512195	27.575069824985064	20.14426389978171
74-75	20.37406818589293	30.99570811074451	26.936979499027508	21.693244204335056
76-77	21.525596263311783	29.90331990507031	27.64232443487859	20.92875939673932
78-79	19.968586191689656	30.220304519356837	28.140467391923156	21.670641897030347
80-81	19.025789572518992	29.625889328567023	28.57165313785453	22.776667961059456
82-83	20.09850697315169	29.53488530178754	28.8498909396187	21.516716785442075
84-85	19.395413770923067	29.006875010090315	28.349581220031762	23.248129998954855
86	19.07388320385157	29.775990741551112	28.85753833682706	22.292587717770257
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	466.0
1	538.5
2	5181.0
3	6148.5
4	2692.0
5	3176.0
6	3791.5
7	4069.0
8	4205.5
9	4403.0
10	4472.0
11	4494.0
12	4611.5
13	4764.0
14	4813.0
15	4786.5
16	4774.5
17	4885.5
18	5010.5
19	5216.0
20	5533.5
21	5674.0
22	5795.0
23	6120.5
24	6948.5
25	8057.0
26	8979.0
27	10626.0
28	12923.5
29	14011.0
30	16474.5
31	19863.5
32	21485.0
33	22820.5
34	25482.5
35	28192.0
36	28879.0
37	30593.0
38	34491.5
39	37791.0
40	41061.5
41	45887.5
42	49388.5
43	50219.0
44	51634.5
45	54287.5
46	55640.5
47	56778.0
48	57726.0
49	54531.5
50	51411.0
51	48301.0
52	43858.0
53	40608.5
54	36867.0
55	32553.0
56	28429.5
57	26795.0
58	24027.5
59	19058.5
60	15338.0
61	12526.0
62	9996.5
63	7328.5
64	5897.0
65	4862.5
66	3314.5
67	2442.5
68	1695.0
69	1091.5
70	776.0
71	675.0
72	545.0
73	282.5
74	148.5
75	136.0
76	85.5
77	35.5
78	23.0
79	21.0
80	16.5
81	9.5
82	7.0
83	5.0
84	2.5
85	2.0
86	2.0
87	1.0
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1176871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.23379013181775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.38200798114372	18.267917611300476
2	6.6191183362033525	3.208126492344374
3	2.8007036506123995	2.036149934710701
4	1.7226833585071333	1.6698858789454727
5	1.1549997824438427	1.399501116501962
6	0.9336732968230947	1.3575865636135953
7	0.7928846510945112	1.3450220163354747
8	0.7066069756825203	1.3699012123494914
9	0.5905697315665444	1.2880568639688819
>10	8.073314121535988	45.9500558701312
>50	1.1647074425430688	18.12247798973579
>100	0.05660787647597572	2.1206674958879166
>500	0.0017689961398734684	0.2518153325552272
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	3.5379922797469367E-4	1.6128356216194684
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18811	1.598390987627361	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0203080881422008	0.0
2	0.0	0.0	0.0	0.06228380170808865	0.0
3	0.0	0.0	0.0	0.11462598704530913	0.0
4	0.0	0.0	0.0	0.17104678422698835	0.0
5	0.0	0.0	0.0	0.31949126114926785	0.0
6	0.0	0.0	0.0	0.5201929523286749	0.0
7	0.0	0.0	0.0	0.6549570853559991	0.0
8	0.0	0.0	0.0	0.8505605117298327	0.0
9	0.0	0.0	0.0	0.9838801363955778	0.0
10	0.0	0.0	0.0	1.1560315446637737	0.0
11	0.0	0.0	0.0	1.378230919106682	0.0
12	0.0	0.0	0.0	1.527015280349333	0.0
13	0.0	0.0	0.0	1.5981360743870823	0.0
14	0.0	0.0	0.0	1.641726238474735	0.0
15	0.0	0.0	0.0	1.688290390365639	0.0
16	0.0	0.0	0.0	1.7679082924126774	0.0
17	0.0	0.0	0.0	1.858912319192163	0.0
18	0.0	0.0	0.0	1.971923855715707	0.0
19	0.0	0.0	0.0	2.0499273072409805	0.0
20	0.0	0.0	0.0	2.1320943416908054	0.0
21	0.0	0.0	0.0	2.2346544353629243	0.0
22	0.0	0.0	0.0	2.3552283980147357	0.0
23	0.0	0.0	0.0	2.4703642115406024	0.0
24	0.0	0.0	0.0	2.564002341802967	0.0
25	0.0	0.0	0.0	2.6459144630125135	0.0
26	0.0	0.0	0.0	2.7351340971100484	0.0
27	0.0	0.0	0.0	2.8444918771895984	0.0
28	0.0	0.0	0.0	2.9443328962987447	0.0
29	0.0	0.0	0.0	3.070769863476966	0.0
30	8.497108009289038E-5	0.0	0.0	3.200945558179274	0.0
31	8.497108009289038E-5	0.0	0.0	3.3195651859889486	0.0
32	8.497108009289038E-5	0.0	0.0	3.427138573386548	0.0
33	8.497108009289038E-5	0.0	0.0	3.5361564691457263	0.0
34	8.497108009289038E-5	0.0	0.0	3.6632732049646903	0.0
35	8.497108009289038E-5	0.0	0.0	3.796847742870714	0.0
36	8.497108009289038E-5	0.0	0.0	3.9274482929734864	0.0
37	8.497108009289038E-5	0.0	0.0	4.079206642019389	0.0
38	8.497108009289038E-5	0.0	0.0	4.2510181659672135	0.0
39	8.497108009289038E-5	0.0	0.0	4.599739478668435	0.0
40	8.497108009289038E-5	0.0	0.0	4.796702442323755	0.0
41	8.497108009289038E-5	0.0	0.0	5.016267713283784	0.0
42	8.497108009289038E-5	0.0	0.0	5.215779809341891	0.0
43	8.497108009289038E-5	0.0	0.0	5.372891336433645	0.0
44	8.497108009289038E-5	0.0	0.0	5.51385835830775	0.0
45	8.497108009289038E-5	0.0	0.0	5.664257170072166	0.0
46	8.497108009289038E-5	0.0	0.0	5.83224499541581	0.0
47	8.497108009289038E-5	0.0	0.0	6.013998135734503	0.0
48	8.497108009289038E-5	0.0	0.0	6.175953014391552	0.0
49	8.497108009289038E-5	0.0	0.0	6.335188818485628	0.0
50	8.497108009289038E-5	0.0	0.0	6.506235602712617	0.0
51	8.497108009289038E-5	0.0	0.0	6.685864466028987	0.0
52	8.497108009289038E-5	0.0	0.0	6.899736674622792	0.0
53	8.497108009289038E-5	0.0	0.0	7.080215248740092	0.0
54	8.497108009289038E-5	0.0	0.0	7.262478215539341	0.0
55	8.497108009289038E-5	0.0	0.0	7.446270661780263	0.0
56	8.497108009289038E-5	0.0	0.0	7.628533628579513	0.0
57	8.497108009289038E-5	0.0	0.0	7.812580988060714	0.0
58	8.497108009289038E-5	0.0	0.0	7.996033549981264	0.0
59	8.497108009289038E-5	0.0	0.0	8.18789824883101	0.0
60	8.497108009289038E-5	0.0	0.0	8.393528262655805	0.0
61	8.497108009289038E-5	0.0	0.0	8.614707984137599	0.0
62	8.497108009289038E-5	0.0	0.0	8.905733933455748	0.0
63	8.497108009289038E-5	0.0	0.0	9.13065238246163	0.0
64	8.497108009289038E-5	0.0	0.0	9.35353152554528	0.0
65	8.497108009289038E-5	0.0	0.0	9.561370787452491	0.0
66	8.497108009289038E-5	0.0	0.0	9.774393285245367	0.0
67	8.497108009289038E-5	0.0	0.0	9.969147000818271	0.0
68	8.497108009289038E-5	0.0	0.0	10.222615732735363	0.0
69	8.497108009289038E-5	0.0	0.0	10.604900622073277	0.0
70	8.497108009289038E-5	0.0	0.0	10.850212130301452	0.0
71	8.497108009289038E-5	0.0	0.0	11.087876241321267	0.0
72	8.497108009289038E-5	0.0	0.0	11.306251917159994	0.0
73	8.497108009289038E-5	0.0	0.0	11.533209672088105	0.0
74	1.6994216018578076E-4	0.0	0.0	11.788037941286683	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9055	0.0	76.99613	1
TAGGGCG	220	0.0	67.27273	5
TACGGGA	490	0.0	64.48979	4
TTAGCGG	405	0.0	64.197525	2
TAGGGAC	1230	0.0	62.764233	5
TAGCGGG	875	0.0	62.62857	3
CCGATCC	680	0.0	61.764706	80
AGGGATC	1400	0.0	61.428566	6
AGCGGGA	890	0.0	60.22472	4
TTGCGGG	795	0.0	59.87421	3
AGGGATT	2110	0.0	59.715637	6
CGAGGGA	735	0.0	58.775513	4
ATAGGGA	1560	0.0	58.717953	4
TATAGCG	75	0.0	58.66666	1
GTAGGGA	2020	0.0	58.613857	4
CGGGACT	480	0.0	58.333332	6
CGAACGG	55	7.003109E-10	58.181816	2
ATAGCGG	200	0.0	58.0	2
TAGGGAT	1920	0.0	57.916664	5
GCTACGG	215	0.0	57.674416	2
>>END_MODULE