FastQCFastQC Report
Thu 26 May 2016
SRR1547845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547845_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2767085
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122220.4416922501477186No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA27720.1001776237448434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71850.072.038971
TGCGTAG2950.070.508481
ATACGCG700.068.571431
TAGGGAC34850.066.800575
TACGGGA11050.065.8823554
ACGGGAT13500.065.777785
GGGCGAT21300.064.7887347
ATAGGGA45400.064.669614
CCGATCC24350.063.90143680
TAGCGGG19250.062.961043
ATTGCGG6100.062.950822
TTACGGG10900.062.385323
ATTCGCG1350.062.222221
CGGGATA7800.062.0512856
CGGGATC9950.061.909556
TAGGGAG45650.061.861995
GGCGATT7800.061.5384648
GTACGGG10400.061.538463
TATGGGA40300.061.439214
TCTAGCG1700.061.176471
Produced by FastQC (version 0.11.5)