##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547845_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2767085
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.067884795732695	33.0	31.0	34.0	31.0	34.0
2	32.50149091914415	34.0	31.0	34.0	31.0	34.0
3	32.62299676374235	34.0	31.0	34.0	31.0	34.0
4	36.144172658230595	37.0	35.0	37.0	35.0	37.0
5	36.102203943861504	37.0	35.0	37.0	35.0	37.0
6	36.163787885084844	37.0	36.0	37.0	35.0	37.0
7	36.373867083952966	37.0	37.0	37.0	35.0	37.0
8	36.37443446804128	37.0	37.0	37.0	35.0	37.0
9	38.18243928177125	39.0	39.0	39.0	37.0	39.0
10-11	37.76628582063796	39.0	37.5	39.0	35.0	39.0
12-13	37.18325801339677	39.0	37.0	39.0	34.5	39.0
14-15	38.33757672785621	40.0	37.0	41.0	33.5	41.0
16-17	38.494440358716844	40.0	37.0	41.0	34.0	41.0
18-19	38.39615154576025	40.0	37.0	41.0	34.0	41.0
20-21	38.25361978399651	40.0	36.0	41.0	34.0	41.0
22-23	38.08774450369252	40.0	35.5	41.0	34.0	41.0
24-25	38.07478339118603	40.0	35.0	41.0	34.0	41.0
26-27	38.074322075397035	40.0	35.0	41.0	34.0	41.0
28-29	37.99364439473309	40.0	36.0	41.0	34.0	41.0
30-31	37.84954491820815	40.0	35.5	41.0	34.0	41.0
32-33	37.62073879190556	40.0	35.0	41.0	33.0	41.0
34-35	37.371626639586424	40.0	35.0	41.0	33.0	41.0
36-37	37.22688207987828	40.0	35.0	41.0	33.0	41.0
38-39	37.13208629297618	40.0	35.0	41.0	32.0	41.0
40-41	37.041506314406675	40.0	35.0	41.0	32.0	41.0
42-43	36.99842017863564	40.0	35.0	41.0	32.0	41.0
44-45	36.867761922745416	39.5	35.0	41.0	32.0	41.0
46-47	36.74332429253167	39.0	35.0	41.0	31.0	41.0
48-49	36.64491296797894	39.0	35.0	41.0	31.0	41.0
50-51	36.430859008667966	39.0	35.0	40.5	31.0	41.0
52-53	36.243982566491454	38.0	35.0	40.0	31.0	41.0
54-55	36.05610434807749	38.0	35.0	40.0	31.0	41.0
56-57	35.77083230186279	37.5	35.0	40.0	30.0	41.0
58-59	35.51126564597763	37.0	35.0	40.0	29.0	41.0
60-61	35.15936120502261	36.5	34.5	40.0	28.5	41.0
62-63	34.75831696532633	36.0	34.0	39.0	28.0	41.0
64-65	34.367936836056714	35.5	34.0	39.0	27.5	40.0
66-67	34.02812092870295	35.0	34.0	38.5	26.5	40.0
68-69	33.518712110397765	35.0	33.5	37.5	25.5	40.0
70-71	33.172692743446625	35.0	33.0	37.0	25.0	39.0
72-73	32.87027106142385	35.0	33.0	36.5	25.0	39.0
74-75	32.545235328874966	35.0	33.0	36.0	24.0	38.5
76-77	30.618337889873278	33.0	30.0	34.5	20.0	36.0
78-79	31.62846479237176	34.5	32.0	35.0	21.0	37.0
80-81	31.678157700251347	35.0	32.0	35.0	20.0	36.5
82-83	31.44966291241505	35.0	32.0	35.0	19.5	36.0
84-85	31.205576445971122	34.0	32.0	35.0	19.0	36.0
86	30.76674406460228	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	3.0
11	18.0
12	27.0
13	62.0
14	228.0
15	748.0
16	1863.0
17	4221.0
18	9054.0
19	14228.0
20	17904.0
21	18972.0
22	17203.0
23	15709.0
24	15789.0
25	17636.0
26	21308.0
27	26176.0
28	32889.0
29	42008.0
30	53669.0
31	69494.0
32	85084.0
33	112839.0
34	260585.0
35	244456.0
36	244382.0
37	460239.0
38	675449.0
39	304121.0
40	710.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.43210273627301	24.618108948586688	30.858827972396945	13.090960342743358
2	29.81075753003612	25.593684328453953	31.34985011302508	13.245708028484849
3	28.90265387582962	25.23756950003343	32.60232338363296	13.257453240503997
4	27.44360943014038	26.58378763211105	31.201643606900404	14.770959330848166
5	24.30279517976499	31.236698547388315	29.962830921348637	14.497675351498057
6	22.180092046323114	41.360312386500595	25.697764976500544	10.761830590675746
7	89.35804285014736	3.2275119846336486	5.736686802176298	1.6777583630426967
8	90.33408803849538	2.677149418973396	5.296223281901351	1.6925392606298686
9	85.83054008098775	5.574819710995506	6.367205922477987	2.227434285538753
10-11	36.34329989862978	30.86903727207513	19.851594728748843	12.936068100546242
12-13	29.619346713237938	23.195095199460805	28.855618819082174	18.329939268219082
14-15	18.242572960353584	30.34127972216249	33.10507266672328	18.311074650760638
16-17	24.281888702370907	27.192858188310083	29.703334013953313	18.8219190953657
18-19	24.61070043023615	28.923235101198554	28.3635847832647	18.102479685300597
20-21	26.761592072523975	27.986400851437526	28.50761722173334	16.74438985430516
22-23	23.020543279299336	23.87693186150769	32.38286861444444	20.719656244748535
24-25	21.576948304804514	27.67005711786953	31.590410124734152	19.16258445259181
26-27	20.072820314518708	31.743513480792963	28.470936021119698	19.71273018356863
28-29	18.47429695871287	28.542672162221255	33.07632400161181	19.906706877454074
30-31	23.129683403292635	27.37402356631618	29.578563723196073	19.917729307195117
32-33	24.841358324735236	27.462582464940542	28.159290372359358	19.536768837964864
34-35	20.44012019869285	27.422919787429734	30.151784278401276	21.985175735476144
36-37	22.793571574418568	29.020503526274037	28.786358207283115	19.39956669202428
38-39	20.792205515912958	28.871809142111644	28.569469315181863	21.76651602679354
40-41	21.14120093889418	26.62180597993918	29.249661647546066	22.98733143362058
42-43	21.25849043307307	26.859059262725935	28.488337004464988	23.394113299736002
44-45	21.124468529156136	28.277935083309693	28.45125827359839	22.146338113935784
46-47	21.647708689830633	27.39460479168511	28.75141168413692	22.206274834347337
48-49	20.535021511807553	26.753352354553616	30.79303671553277	21.91858941810606
50-51	19.595910497870502	28.257661040408948	29.448752026049075	22.697676435671475
52-53	21.25933970224984	25.84671233446027	30.21750325703764	22.676444706252248
54-55	21.240095624095392	25.73484370736714	31.05556930849612	21.969491360041342
56-57	21.955270618719698	26.298595814729218	29.511941266712082	22.234192299839002
58-59	22.38586816089856	27.2614863656158	28.388665328314815	21.96398014517082
60-61	23.14327171012094	28.094438732456716	27.728457925940113	21.03383163148223
62-63	22.418321085185312	26.618698016143345	29.558108984725806	21.40487191394554
64-65	21.685383716076664	28.494625210284468	29.263611345513418	20.556379728125446
66-67	20.252214875943455	28.683470149995394	27.678007722928644	23.38630725113251
68-69	19.511471458231316	30.017906930939958	27.724554901638363	22.74606670919036
70-71	20.13004660138738	28.727758634086054	29.239958295462554	21.902236469064015
72-73	21.526208988881802	29.34593624698916	28.00157927927765	21.126275484851387
74-75	20.089263611345515	30.72854646676918	26.775216518466184	22.406973403419123
76-77	21.70047179613203	28.41766696722363	27.618866063023003	22.262995173621338
78-79	19.91212774453983	28.40474723400257	28.762813574573965	22.920311446883634
80-81	18.524819439952154	28.464286424161166	28.731553241046083	24.279340894840598
82-83	19.814353371869675	28.240386543962327	29.208065527441335	22.737194556726664
84-85	18.67331505898807	27.92951788615095	28.562964274678947	24.834202780182032
86	19.33279967908467	28.064407128801612	28.6243104205328	23.97848277158092
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	784.0
1	1077.0
2	4379.0
3	4995.5
4	2777.0
5	3295.5
6	3851.5
7	4063.0
8	4166.0
9	4326.0
10	4411.5
11	4534.5
12	4558.5
13	4592.5
14	4697.0
15	4614.5
16	4754.5
17	4927.0
18	5222.0
19	5502.5
20	6215.5
21	6993.0
22	7574.0
23	8998.0
24	10317.0
25	13541.5
26	17242.5
27	21292.0
28	29682.5
29	34976.0
30	37947.5
31	43771.5
32	46993.0
33	50050.5
34	56579.0
35	64713.5
36	69008.0
37	72412.5
38	78838.5
39	89895.0
40	102808.5
41	114947.0
42	126650.5
43	131094.0
44	131908.0
45	135634.0
46	142506.0
47	147694.0
48	153853.0
49	148706.5
50	138629.0
51	127976.5
52	108973.5
53	97728.0
54	91599.0
55	85760.0
56	77684.0
57	72213.0
58	65125.5
59	48930.5
60	34567.5
61	26669.5
62	21165.0
63	15473.5
64	12644.0
65	10423.5
66	6924.5
67	4947.0
68	3372.0
69	2239.5
70	1469.0
71	955.0
72	836.5
73	597.0
74	465.0
75	377.0
76	216.5
77	159.5
78	120.5
79	55.0
80	42.5
81	26.0
82	19.5
83	14.5
84	10.5
85	7.5
86	6.0
87	5.0
88	4.0
89	3.0
90	1.0
91	1.5
92	1.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2767085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.697053294412555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42213374361486	13.701436270278888
2	9.39643198338394	3.325783151745364
3	2.979423856578591	1.5818106832954653
4	1.4958716719821419	1.058900828026799
5	0.8849213624951089	0.7830250256720057
6	0.5955955948110998	0.6324172189973628
7	0.43674960327359685	0.5410426703812469
8	0.3189395294683298	0.45154318805567173
9	0.27331870686576104	0.4353242149586966
>10	3.213766586187456	13.834581416068053
>50	1.4185086996309866	18.227153142376853
>100	1.5571221276450138	43.8397850611333
>500	0.004948480500396008	0.5882751417055081
>1k	0.0020618668751650033	0.5529538207049541
>5k	0.0	0.0
>10k+	2.0618668751650032E-4	0.44596816659997024
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12222	0.4416922501477186	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	2772	0.1001776237448434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4455645562026464E-4	0.0	0.0	0.0523294369345358	0.0
2	1.4455645562026464E-4	0.0	0.0	0.15984330080210762	0.0
3	1.4455645562026464E-4	0.0	0.0	0.24610736569350056	0.0
4	1.4455645562026464E-4	0.0	0.0	0.40002385181517736	0.0
5	1.4455645562026464E-4	0.0	0.0	0.7418998693571033	0.0
6	1.4455645562026464E-4	0.0	0.0	1.0306152503446768	0.0
7	1.4455645562026464E-4	0.0	0.0	1.2135153058182166	0.0
8	1.4455645562026464E-4	0.0	0.0	1.548561030832085	0.0
9	1.4455645562026464E-4	0.0	0.0	1.6638809433031512	0.0
10	1.4455645562026464E-4	0.0	0.0	1.923251363799811	0.0
11	1.4455645562026464E-4	0.0	0.0	2.3688466382492765	0.0
12	1.4455645562026464E-4	0.0	0.0	2.751885106529073	0.0
13	1.4455645562026464E-4	0.0	0.0	2.89210486848073	0.0
14	1.4455645562026464E-4	0.0	0.0	2.9482289123752974	0.0
15	1.4455645562026464E-4	0.0	0.0	3.0367697414427095	0.0
16	1.4455645562026464E-4	0.0	0.0	3.2132008955272426	0.0
17	1.4455645562026464E-4	0.0	0.0	3.44109414781259	0.0
18	1.4455645562026464E-4	0.0	0.0	3.710041433494092	0.0
19	1.4455645562026464E-4	0.0	0.0	3.8944593317516447	0.0
20	1.4455645562026464E-4	0.0	0.0	4.097235899872971	0.0
21	1.4455645562026464E-4	0.0	0.0	4.343379404680377	0.0
22	1.4455645562026464E-4	0.0	0.0	4.610375178211005	0.0
23	1.4455645562026464E-4	0.0	0.0	4.86970945959376	0.0
24	1.4455645562026464E-4	0.0	0.0	5.056367982913427	0.0
25	1.4455645562026464E-4	0.0	0.0	5.2250653666222755	0.0
26	1.4455645562026464E-4	0.0	0.0	5.382234372995408	0.0
27	1.4455645562026464E-4	0.0	0.0	5.570519156440803	0.0
28	1.4455645562026464E-4	0.0	0.0	5.758406409633242	0.0
29	1.4455645562026464E-4	0.0	0.0	5.987058583310596	0.0
30	1.4455645562026464E-4	0.0	0.0	6.220264285339987	0.0
31	1.4455645562026464E-4	0.0	0.0	6.43706282965648	0.0
32	1.4455645562026464E-4	0.0	0.0	6.6305516455042035	0.0
33	1.4455645562026464E-4	0.0	0.0	6.826389503755758	0.0
34	1.4455645562026464E-4	0.0	0.0	7.040802866554515	0.0
35	1.4455645562026464E-4	0.0	0.0	7.276429889215546	0.0
36	1.4455645562026464E-4	0.0	0.0	7.492686346823462	0.0
37	1.4455645562026464E-4	0.0	0.0	7.735252079354266	0.0
38	1.8069556952533082E-4	0.0	0.0	8.003874113010623	0.0
39	1.8069556952533082E-4	0.0	0.0	8.441374225945355	0.0
40	1.8069556952533082E-4	0.0	0.0	8.71220074554992	0.0
41	1.8069556952533082E-4	0.0	0.0	8.997627467172132	0.0
42	1.8069556952533082E-4	0.0	0.0	9.279837807656794	0.0
43	1.8069556952533082E-4	0.0	0.0	9.51792229006337	0.0
44	1.8069556952533082E-4	0.0	0.0	9.742635300325071	0.0
45	1.8069556952533082E-4	0.0	0.0	9.974684550709501	0.0
46	1.8069556952533082E-4	0.0	0.0	10.246306130819978	0.0
47	2.5297379733546315E-4	0.0	0.0	10.53664777193328	0.0
48	2.5297379733546315E-4	0.0	0.0	10.777948635477406	0.0
49	2.5297379733546315E-4	0.0	0.0	11.026007513321781	0.0
50	2.5297379733546315E-4	0.0	0.0	11.283354143439757	0.0
51	2.5297379733546315E-4	0.0	0.0	11.55537325380319	0.0
52	2.5297379733546315E-4	0.0	0.0	11.875999472368937	0.0
53	2.5297379733546315E-4	0.0	0.0	12.128575739451444	0.0
54	2.5297379733546315E-4	0.0	0.0	12.392029879819377	0.0
55	2.5297379733546315E-4	0.0	0.0	12.654941933478733	0.0
56	2.5297379733546315E-4	0.0	0.0	12.92656351358921	0.0
57	2.5297379733546315E-4	0.0	0.0	13.19493257344823	0.0
58	2.891129112405293E-4	0.0	0.0	13.442196390786695	0.0
59	2.891129112405293E-4	0.0	0.0	13.716709100009577	0.0
60	2.891129112405293E-4	0.0	0.0	14.005532898338865	0.0
61	2.891129112405293E-4	0.0	0.0	14.304909317928434	0.0
62	3.2525202514559545E-4	0.0	0.0	14.665107866220229	0.0
63	3.2525202514559545E-4	0.0	0.0	14.989203439720862	0.0
64	3.6139113905066163E-4	0.0	0.0	15.283411966022005	0.0
65	4.3366936686079394E-4	0.0	0.0	15.572669433718154	0.0
66	4.698084807658601E-4	0.0	0.0	15.854879774202816	0.0
67	5.059475946709263E-4	0.0	0.0	16.13882479215492	0.0
68	5.059475946709263E-4	0.0	0.0	16.469353127930656	0.0
69	5.059475946709263E-4	0.0	0.0	16.915671184658223	0.0
70	5.059475946709263E-4	0.0	0.0	17.209771293617653	0.0
71	5.059475946709263E-4	0.0	0.0	17.501992168654017	0.0
72	5.059475946709263E-4	0.0	0.0	17.778709363825108	0.0
73	5.059475946709263E-4	0.0	0.0	18.07118321265881	0.0
74	5.059475946709263E-4	0.0	0.0	18.386099451227555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7185	0.0	72.03897	1
TGCGTAG	295	0.0	70.50848	1
ATACGCG	70	0.0	68.57143	1
TAGGGAC	3485	0.0	66.80057	5
TACGGGA	1105	0.0	65.882355	4
ACGGGAT	1350	0.0	65.77778	5
GGGCGAT	2130	0.0	64.788734	7
ATAGGGA	4540	0.0	64.66961	4
CCGATCC	2435	0.0	63.901436	80
TAGCGGG	1925	0.0	62.96104	3
ATTGCGG	610	0.0	62.95082	2
TTACGGG	1090	0.0	62.38532	3
ATTCGCG	135	0.0	62.22222	1
CGGGATA	780	0.0	62.051285	6
CGGGATC	995	0.0	61.90955	6
TAGGGAG	4565	0.0	61.86199	5
GGCGATT	780	0.0	61.538464	8
GTACGGG	1040	0.0	61.53846	3
TATGGGA	4030	0.0	61.43921	4
TCTAGCG	170	0.0	61.17647	1
>>END_MODULE