FastQCFastQC Report
Thu 26 May 2016
SRR1547844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547844_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2271025
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363651.6012593432480928No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG451.8189894E-1280.01
CGTTTTT146800.076.103541
CGGGTAT1750.070.857156
CGTAAGG3750.070.3999942
GCTACGG2000.070.02
TACGGGA11300.069.734514
GCGTAAG2400.068.3333361
CCGTTCG650.067.692311
ACGGGAT12200.067.5409855
GTCGACG600.066.666671
GTACGGG7700.065.974033
CGGGATT13650.065.641036
TGCGGGA19450.065.398464
TAGCGGG13050.064.980843
CGGGACT9050.064.9723746
CGGGATA8150.063.8036776
CCGATCC12550.063.74501880
CTAGCGG2400.063.3333362
CTACGGG4050.063.2098733
AGGGATT49600.063.1451576
Produced by FastQC (version 0.11.5)