FastQCFastQC Report
Thu 26 May 2016
SRR1547843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547843_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2288077
Sequences flagged as poor quality0
Sequence length86
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340831.4895914779091788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127000.075.716541
TAACGGG7600.070.03
GGGCGAT14900.067.9194647
CGAGGGA19350.067.803624
TACGGGA11900.066.554624
GTAGGGA36900.065.1490554
GTAGGGT21550.064.779584
GCGAGGG20700.064.347833
AGGGATT51150.064.2815256
GTTTGCG2000.064.01
GCGCGAC950.063.1578949
ACGGGAT14400.063.0555535
CCGATCC16700.062.99401580
TACGCGG1150.062.6086962
CGAAGGG12650.062.6086923
GTACGGG8950.062.1229063
TTACGGG7300.061.9178123
ATAGGGA37250.061.852354
AGGGTAA22900.061.659396
TAGGGTA22350.061.5659945
Produced by FastQC (version 0.11.5)