##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547843_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2288077
Sequences flagged as poor quality	0
Sequence length	86
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06559219816466	33.0	31.0	34.0	31.0	34.0
2	32.496065910369275	34.0	31.0	34.0	31.0	34.0
3	32.56824136600298	34.0	31.0	34.0	31.0	34.0
4	36.12434065811596	37.0	35.0	37.0	35.0	37.0
5	36.09295360252299	37.0	35.0	37.0	35.0	37.0
6	36.14483909413888	37.0	36.0	37.0	35.0	37.0
7	36.35889001987258	37.0	37.0	37.0	35.0	37.0
8	36.36028682601154	37.0	37.0	37.0	35.0	37.0
9	38.14481593058276	39.0	39.0	39.0	37.0	39.0
10-11	37.76009723448992	39.0	37.5	39.0	35.0	39.0
12-13	37.17007884787094	39.0	37.0	39.0	34.5	39.0
14-15	38.401507903798695	40.0	37.0	41.0	34.0	41.0
16-17	38.51973447571913	40.0	37.0	41.0	34.5	41.0
18-19	38.335740230770206	40.0	37.0	41.0	34.0	41.0
20-21	38.12986188838924	40.0	35.0	41.0	34.0	41.0
22-23	37.93193869786725	40.0	35.0	41.0	34.0	41.0
24-25	37.8641072831028	40.0	35.0	41.0	34.0	41.0
26-27	37.81001338678725	40.0	35.0	41.0	34.0	41.0
28-29	37.70660843144702	40.0	35.0	41.0	34.0	41.0
30-31	37.53314333390004	40.0	35.0	41.0	33.0	41.0
32-33	37.17302171211895	40.0	35.0	41.0	33.0	41.0
34-35	36.82318558335231	40.0	35.0	41.0	31.5	41.0
36-37	36.61978049689762	40.0	35.0	41.0	31.0	41.0
38-39	36.52552754999067	39.0	35.0	41.0	31.0	41.0
40-41	36.404679125746206	39.0	35.0	41.0	31.0	41.0
42-43	36.340265865178495	39.0	35.0	41.0	30.5	41.0
44-45	36.197161415459355	39.0	35.0	41.0	30.0	41.0
46-47	36.05395819284054	39.0	35.0	41.0	30.0	41.0
48-49	35.9314310663496	38.0	35.0	41.0	29.5	41.0
50-51	35.70879410963879	38.0	35.0	40.0	29.0	41.0
52-53	35.533134811459576	38.0	35.0	40.0	28.0	41.0
54-55	35.33852291684239	37.5	35.0	40.0	28.0	41.0
56-57	35.06350660401726	37.0	34.5	40.0	27.5	41.0
58-59	34.79171964055406	36.5	34.0	40.0	26.5	41.0
60-61	34.42354431253843	36.0	34.0	39.5	26.0	41.0
62-63	34.007274667766865	35.5	33.5	39.0	25.5	40.5
64-65	33.61976454463726	35.0	33.0	39.0	24.5	40.0
66-67	33.26924727620617	35.0	33.0	38.0	22.5	40.0
68-69	32.81016460547438	35.0	33.0	37.0	21.0	40.0
70-71	32.497506858379325	35.0	33.0	37.0	20.0	39.0
72-73	32.22013135921562	35.0	33.0	36.0	19.0	39.0
74-75	31.894699347967745	35.0	32.5	36.0	18.5	38.5
76-77	29.96281331441206	33.0	29.5	34.5	16.0	36.0
78-79	30.98319680675082	34.0	31.0	35.0	17.0	37.0
80-81	31.125670595875924	35.0	32.0	35.0	16.5	36.5
82-83	30.93716579468261	35.0	32.0	35.0	15.0	36.0
84-85	30.691744639712738	34.0	31.0	35.0	12.0	36.0
86	30.2722679350389	34.0	31.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	9.0
11	16.0
12	37.0
13	95.0
14	289.0
15	952.0
16	2686.0
17	6468.0
18	13828.0
19	21252.0
20	23620.0
21	22378.0
22	18843.0
23	16139.0
24	15687.0
25	17226.0
26	20191.0
27	24427.0
28	29385.0
29	37085.0
30	47216.0
31	59220.0
32	71204.0
33	89693.0
34	217681.0
35	224493.0
36	192500.0
37	349001.0
38	520089.0
39	245728.0
40	627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.059303074153537	24.443102220773163	29.6035491812557	15.8940455238176
2	33.47417940917198	24.52950665558895	29.167156524889677	12.82915741034939
3	28.321118563754627	24.654371334531135	34.07660668762459	12.947903414089648
4	27.0147377033203	25.4906194153431	33.47321790306882	14.021424978267778
5	23.402796321976925	31.05808939122241	31.606322689315085	13.932791597485576
6	21.71705759902311	39.845555896938784	28.346511065842627	10.09087543819548
7	86.21969452951103	3.5090165234823827	8.757878340632768	1.513410606373824
8	86.58095859536196	3.2460446042681257	8.410774637392011	1.7622221629779067
9	81.83754305471363	6.316352115772328	9.540456898959256	2.3056479305547843
10-11	35.53234878022025	30.639266073650496	22.19604060527683	11.632344540852428
12-13	27.211387553827954	26.718921609718553	29.859812410159275	16.209878426294217
14-15	16.14381858652484	32.87006949503885	34.25955507616221	16.7265568422741
16-17	20.569128573907257	28.70320797770355	32.88956184603927	17.83810160234992
18-19	22.08981603328909	31.046048712521472	30.049600603476197	16.81453465071324
20-21	25.128568662680493	29.246917826629087	29.71536797057092	15.909145540119498
22-23	21.378454483830744	26.90364004358245	31.75786916261996	19.960036309966842
24-25	19.1223241175887	29.782585988146376	33.29689079519614	17.798199099068782
26-27	18.03612815477801	34.81923903784707	29.23867946751792	17.905953339857007
28-29	16.698323526699497	29.54620845364907	34.56546698384713	19.1900010358043
30-31	21.094635364107063	29.59212037007496	30.66157738572609	18.651666880091884
32-33	23.1889049188467	29.296151309593167	29.369094658964713	18.145849112595425
34-35	18.841170992060142	29.5475195983352	30.748287754301973	20.863021655302685
36-37	20.883344397937655	31.176813542551233	29.851836279985335	18.088005779525776
38-39	19.270352352652466	32.176561365723266	29.038380264300546	19.514706017323718
40-41	19.556356713519694	28.723006262464068	30.094835095147587	21.62580192886865
42-43	19.975048916623	29.204830082204403	28.182159079436573	22.637961921736025
44-45	18.976022223028334	31.43056374413973	28.73887111316621	20.85454291966573
46-47	20.29909832579935	30.72092853518479	28.439121585506083	20.540851553509782
48-49	19.93296117219831	28.700869769679954	30.866793381516445	20.49937567660529
50-51	18.459278249814144	31.175393135807926	30.390782303217946	19.974546311159983
52-53	20.42654158929092	27.933566046946844	30.49814320060033	21.141749163161904
54-55	19.811439912205753	28.696149648809893	30.658649162593743	20.83376127639061
56-57	20.793989887578086	28.307329692138854	29.7824767260892	21.116203694193857
58-59	21.495321180187553	29.76241621239145	28.76588069370043	19.976381913720562
60-61	21.922841757510785	31.537553150527714	27.393527403142464	19.146077688819037
62-63	21.644004987594386	29.247748218263638	29.843466806405555	19.26477998773643
64-65	19.73919584000014	31.8193399959879	29.19720359061343	19.244260573398535
66-67	18.25644416687026	30.998366750769314	27.499424188958677	23.245764893401752
68-69	17.79699721643983	33.37090491272802	26.879864619940676	21.95223325089147
70-71	18.458032662362324	31.443565928943823	28.990873121839865	21.107528286853984
72-73	20.65594820454032	31.597188381335066	27.7493720709574	19.99749134316721
74-75	19.571107091238627	32.62654622200215	26.555793358352886	21.246553328406343
76-77	21.799616009426256	30.242076643399674	27.80848284389031	20.14982450328376
78-79	18.152514098083238	30.365673882478607	29.435941185545765	22.04587083389239
80-81	16.965884452315198	30.12343990171659	30.186571518353624	22.724104127614588
82-83	18.496471054077286	29.56421484067188	30.356911065492987	21.582403039757843
84-85	17.206588764276727	29.18358953828914	29.089580464293814	24.520241233140318
86	17.847388877209987	29.416361424899602	29.99077391189195	22.74547578599846
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1011.0
1	1366.5
2	11245.0
3	12461.5
4	4664.0
5	6808.5
6	9892.5
7	11341.0
8	11754.5
9	11321.5
10	9693.5
11	8567.5
12	7899.0
13	7660.5
14	7746.0
15	7522.5
16	7279.5
17	7502.0
18	7651.5
19	7962.0
20	8420.0
21	8475.0
22	8973.0
23	10070.5
24	12420.5
25	15748.0
26	18854.0
27	22924.0
28	29484.5
29	33504.0
30	35007.5
31	39249.0
32	46626.0
33	51075.0
34	54730.0
35	63066.5
36	67558.0
37	71413.0
38	77831.0
39	79766.5
40	86861.5
41	97550.0
42	103029.5
43	105543.0
44	109678.5
45	112911.5
46	114280.5
47	119704.5
48	120365.5
49	111294.5
50	104715.0
51	91878.5
52	73153.0
53	62894.5
54	55218.0
55	49046.0
56	44147.5
57	42114.0
58	38959.0
59	30408.0
60	21167.5
61	16246.0
62	13017.5
63	9448.5
64	8031.0
65	6238.5
66	3844.0
67	2722.0
68	2129.5
69	1598.0
70	940.0
71	741.0
72	540.5
73	395.0
74	366.0
75	221.0
76	124.0
77	64.5
78	71.0
79	101.0
80	70.5
81	29.0
82	14.0
83	8.5
84	6.5
85	7.0
86	8.0
87	5.0
88	1.5
89	2.0
90	1.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2288077.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.772771830630962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98934259974237	14.828489056765019
2	8.302230815031297	3.1171176955163173
3	2.858141972393992	1.6096574132190584
4	1.5154171151958837	1.1379431892722127
5	0.91112451525464	0.8552166317084797
6	0.6147555466738894	0.6924399365594237
7	0.44196905812602355	0.5807888999079099
8	0.33893095718430705	0.5090138820446678
9	0.27207507283654714	0.4596842936846519
>10	3.116611434433839	13.782942605586381
>50	1.1761678102672792	15.963771891666969
>100	1.455492369903776	43.70983148448323
>500	0.00562962396818304	0.7266631376529972
>1k	0.0018765413227276803	0.5256000006681719
>5k	0.0	0.0
>10k+	2.3456766534096004E-4	1.5008398812645196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34083	1.4895914779091788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3704822870908626E-5	0.0	0.0	0.03601277404562871	0.0
2	4.3704822870908626E-5	0.0	0.0	0.1387628126151349	0.0
3	4.3704822870908626E-5	0.0	0.0	0.22822658503188487	0.0
4	4.3704822870908626E-5	0.0	0.0	0.3767792779701033	0.0
5	4.3704822870908626E-5	0.0	0.0	0.7644410568350628	0.0
6	4.3704822870908626E-5	0.0	0.0	1.1306874724932772	0.0
7	4.3704822870908626E-5	0.0	0.0	1.3953201749766289	0.0
8	4.3704822870908626E-5	0.0	0.0	1.8029987627164645	0.0
9	4.3704822870908626E-5	0.0	0.0	1.9629147096011192	0.0
10	4.3704822870908626E-5	0.0	0.0	2.1852411435454315	0.0
11	4.3704822870908626E-5	0.0	0.0	2.539031684685437	0.0
12	4.3704822870908626E-5	0.0	0.0	2.8002554109848576	0.0
13	4.3704822870908626E-5	0.0	0.0	2.9001209312448837	0.0
14	4.3704822870908626E-5	0.0	0.0	2.940853826160571	0.0
15	4.3704822870908626E-5	0.0	0.0	2.999068650224621	0.0
16	4.3704822870908626E-5	0.0	0.0	3.123321461646614	0.0
17	4.3704822870908626E-5	0.0	0.0	3.277818010495276	0.0
18	4.3704822870908626E-5	0.0	0.0	3.4588433868265795	0.0
19	4.3704822870908626E-5	0.0	0.0	3.57335002274836	0.0
20	4.3704822870908626E-5	0.0	0.0	3.698782864387868	0.0
21	4.3704822870908626E-5	0.0	0.0	3.8520556781961446	0.0
22	4.3704822870908626E-5	0.0	0.0	4.017828071345501	0.0
23	4.3704822870908626E-5	0.0	0.0	4.193652573755166	0.0
24	4.3704822870908626E-5	0.0	0.0	4.32703969315718	0.0
25	4.3704822870908626E-5	0.0	0.0	4.438312172186513	0.0
26	4.3704822870908626E-5	0.0	0.0	4.545563807511723	0.0
27	4.3704822870908626E-5	0.0	0.0	4.655962190083638	0.0
28	4.3704822870908626E-5	0.0	0.0	4.77724307355041	0.0
29	4.3704822870908626E-5	0.0	0.0	4.916137000634157	0.0
30	4.3704822870908626E-5	0.0	0.0	5.075615899290103	0.0
31	4.3704822870908626E-5	0.0	0.0	5.223950068113966	0.0
32	4.3704822870908626E-5	0.0	0.0	5.355632699424014	0.0
33	4.3704822870908626E-5	0.0	0.0	5.491510993729669	0.0
34	4.3704822870908626E-5	0.0	0.0	5.632808686071317	0.0
35	4.3704822870908626E-5	0.0	0.0	5.7955654464425805	0.0
36	4.3704822870908626E-5	0.0	0.0	5.944118139380799	0.0
37	4.3704822870908626E-5	0.0	0.0	6.096822790491753	0.0
38	4.3704822870908626E-5	0.0	0.0	6.258006177239665	0.0
39	4.3704822870908626E-5	0.0	0.0	6.46896935723754	0.0
40	8.740964574181725E-5	0.0	0.0	6.652485908472486	0.0
41	8.740964574181725E-5	0.0	0.0	6.838187700850977	0.0
42	8.740964574181725E-5	0.0	0.0	7.0241080173438215	0.0
43	8.740964574181725E-5	0.0	0.0	7.200107339044971	8.740964574181725E-5
44	8.740964574181725E-5	0.0	0.0	7.361771478844462	8.740964574181725E-5
45	8.740964574181725E-5	0.0	0.0	7.531215077114974	8.740964574181725E-5
46	8.740964574181725E-5	0.0	0.0	7.715518315161597	8.740964574181725E-5
47	8.740964574181725E-5	0.0	0.0	7.910878873394558	8.740964574181725E-5
48	8.740964574181725E-5	0.0	0.0	8.101169672174494	8.740964574181725E-5
49	8.740964574181725E-5	0.0	0.0	8.297010983459035	8.740964574181725E-5
50	8.740964574181725E-5	0.0	0.0	8.489661842674002	8.740964574181725E-5
51	8.740964574181725E-5	0.0	0.0	8.691315895400374	8.740964574181725E-5
52	8.740964574181725E-5	0.0	0.0	8.905906575696534	8.740964574181725E-5
53	1.3111446861272587E-4	0.0	0.0	9.10913400204626	8.740964574181725E-5
54	1.3111446861272587E-4	0.0	0.0	9.314852603299627	8.740964574181725E-5
55	1.3111446861272587E-4	0.0	0.0	9.526733584577793	8.740964574181725E-5
56	1.3111446861272587E-4	0.0	0.0	9.738352336918732	8.740964574181725E-5
57	1.3111446861272587E-4	0.0	0.0	9.940967895748264	8.740964574181725E-5
58	1.3111446861272587E-4	0.0	0.0	10.135935110575387	8.740964574181725E-5
59	1.3111446861272587E-4	0.0	0.0	10.345150097658427	8.740964574181725E-5
60	1.3111446861272587E-4	0.0	0.0	10.570492164380832	8.740964574181725E-5
61	1.3111446861272587E-4	0.0	0.0	10.800466942327553	8.740964574181725E-5
62	1.3111446861272587E-4	0.0	0.0	11.064793711050807	8.740964574181725E-5
63	1.748192914836345E-4	0.0	0.0	11.320685448959978	8.740964574181725E-5
64	1.748192914836345E-4	0.0	0.0	11.571201493656027	8.740964574181725E-5
65	1.748192914836345E-4	0.0	0.0	11.812277296611958	8.740964574181725E-5
66	2.1852411435454314E-4	0.0	0.0	12.047977406354768	8.740964574181725E-5
67	2.1852411435454314E-4	0.0	0.0	12.296483029198756	8.740964574181725E-5
68	2.1852411435454314E-4	0.0	0.0	12.54966506808993	8.740964574181725E-5
69	2.1852411435454314E-4	0.0	0.0	12.828938886235035	8.740964574181725E-5
70	2.1852411435454314E-4	0.0	0.0	13.08312613605224	8.740964574181725E-5
71	2.1852411435454314E-4	0.0	0.0	13.329053174346843	8.740964574181725E-5
72	2.1852411435454314E-4	0.0	0.0	13.571483826811772	8.740964574181725E-5
73	2.1852411435454314E-4	0.0	0.0	13.815793786660151	8.740964574181725E-5
74	2.6222893722545174E-4	0.0	0.0	14.081825043475373	8.740964574181725E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12700	0.0	75.71654	1
TAACGGG	760	0.0	70.0	3
GGGCGAT	1490	0.0	67.919464	7
CGAGGGA	1935	0.0	67.80362	4
TACGGGA	1190	0.0	66.55462	4
GTAGGGA	3690	0.0	65.149055	4
GTAGGGT	2155	0.0	64.77958	4
GCGAGGG	2070	0.0	64.34783	3
AGGGATT	5115	0.0	64.281525	6
GTTTGCG	200	0.0	64.0	1
GCGCGAC	95	0.0	63.157894	9
ACGGGAT	1440	0.0	63.055553	5
CCGATCC	1670	0.0	62.994015	80
TACGCGG	115	0.0	62.608696	2
CGAAGGG	1265	0.0	62.608692	3
GTACGGG	895	0.0	62.122906	3
TTACGGG	730	0.0	61.917812	3
ATAGGGA	3725	0.0	61.85235	4
AGGGTAA	2290	0.0	61.65939	6
TAGGGTA	2235	0.0	61.565994	5
>>END_MODULE