FastQCFastQC Report
Thu 26 May 2016
SRR1547842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547842_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1260251
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74290.5894857452999442No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT39600.072.424251
GCGCGAC559.094947E-1265.4545449
GTTAGCG1050.064.761911
TAGGGAC14800.064.59465
TAAGTCG459.424184E-962.222221
AGGGATT22700.062.202656
ATAGGGA18900.061.3756644
ACGGGAT6050.060.1652875
GTAGGGA20800.059.9999964
GGGCGAT9100.059.7802167
ACGAGGG6550.059.2366453
ACGGGAC3850.059.220785
CGAAACG1700.058.82353280
TAACGGG3200.058.753
TACGGGA5250.058.6666684
CGTAGGG7500.058.6666683
CTACGCG750.058.6666681
CGGGATC3350.058.5074656
GCGGGAT9250.057.9459465
CGGGATA2700.057.777786
Produced by FastQC (version 0.11.5)