FastQCFastQC Report
Thu 26 May 2016
SRR1547841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547841_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2020089
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109160.5403722311244702No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT71400.071.5966341
TACGGGA8900.068.314614
GGGCGAT16400.064.390247
TAGCGGG13550.064.354243
TCGATAG1450.063.448281
ACGGGAT13100.062.2900735
TAGGGAC23850.062.054515
CCGATCC14450.062.00691680
CTAGCGG1750.061.7142872
CGAGGGA16950.061.356934
CGGGATT11800.061.016956
TAATGCG1050.060.9523851
CGTTAGG3750.060.82
GTAGGGT13400.059.9999964
CGAAGGG11850.059.7468383
TATGGGA27100.059.6309974
TAAGGGA29500.059.5254254
CGAGGGT3700.059.459464
GCGAGGG21000.059.428573
TAGGGTA14300.059.0209775