##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547841_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2020089
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.99451311303611	33.0	31.0	34.0	31.0	34.0
2	32.41844938515085	33.0	31.0	34.0	31.0	34.0
3	32.54050291843577	34.0	31.0	34.0	31.0	34.0
4	36.07471106471051	37.0	35.0	37.0	35.0	37.0
5	36.04519454340873	37.0	35.0	37.0	35.0	37.0
6	36.09169199970892	37.0	35.0	37.0	35.0	37.0
7	36.316564270188096	37.0	36.0	37.0	35.0	37.0
8	36.319369097104136	37.0	37.0	37.0	35.0	37.0
9	38.09530916707135	39.0	39.0	39.0	37.0	39.0
10-11	37.66121170898906	39.0	37.5	39.0	35.0	39.0
12-13	37.088891628042134	39.0	36.0	39.0	33.5	39.0
14-15	38.282441021162924	40.0	37.0	41.0	33.5	41.0
16-17	38.40283571664417	40.0	37.0	41.0	34.0	41.0
18-19	38.276518757341876	40.0	36.0	41.0	34.0	41.0
20-21	38.11202748987792	40.0	35.5	41.0	34.0	41.0
22-23	37.917123948499295	40.0	35.0	41.0	34.0	41.0
24-25	37.909819319841844	40.0	35.0	41.0	34.0	41.0
26-27	37.910602701168116	40.0	35.0	41.0	34.0	41.0
28-29	37.82510226034596	40.0	35.0	41.0	34.0	41.0
30-31	37.68101628195589	40.0	35.5	41.0	33.5	41.0
32-33	37.39833839004123	40.0	35.0	41.0	33.0	41.0
34-35	37.09734818614427	40.0	35.0	41.0	32.0	41.0
36-37	36.92369172843375	40.0	35.0	41.0	31.5	41.0
38-39	36.83035202904426	40.0	35.0	41.0	31.0	41.0
40-41	36.72570540208872	40.0	35.0	41.0	31.0	41.0
42-43	36.695996810041535	39.5	35.0	41.0	31.0	41.0
44-45	36.55987384714238	39.0	35.0	41.0	31.0	41.0
46-47	36.43321828889717	39.0	35.0	41.0	31.0	41.0
48-49	36.32534729905464	39.0	35.0	41.0	30.5	41.0
50-51	36.079183639928736	38.5	35.0	40.0	30.0	41.0
52-53	35.865939074961545	38.0	35.0	40.0	29.5	41.0
54-55	35.685086647172476	38.0	35.0	40.0	29.0	41.0
56-57	35.36781027964609	37.0	35.0	40.0	28.5	41.0
58-59	35.11323684253516	37.0	34.5	40.0	28.0	41.0
60-61	34.76632613711574	36.0	34.0	39.5	27.5	41.0
62-63	34.3921223272836	36.0	34.0	39.0	26.5	41.0
64-65	34.06891008267458	35.0	34.0	39.0	26.0	40.0
66-67	33.76837332414562	35.0	34.0	38.0	26.0	40.0
68-69	33.334063994210155	35.0	33.5	37.5	25.5	40.0
70-71	33.038514392187665	35.0	33.0	37.0	25.0	39.0
72-73	32.796538419841895	35.0	33.0	36.5	25.0	39.0
74-75	32.48162828469438	35.0	33.0	36.0	24.0	38.5
76-77	30.516991578093837	33.0	30.0	34.5	20.0	36.0
78-79	31.575524890239983	34.0	31.5	35.0	21.5	37.0
80-81	31.712252776981607	35.0	32.0	35.0	21.0	36.5
82-83	31.52013797411896	34.5	32.0	35.0	20.0	36.0
84-85	31.312766912744934	34.0	32.0	35.0	19.5	36.0
86	30.892520577063685	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	7.0
10	12.0
11	21.0
12	36.0
13	70.0
14	227.0
15	765.0
16	2023.0
17	4589.0
18	9248.0
19	13883.0
20	16147.0
21	16143.0
22	14572.0
23	12996.0
24	13094.0
25	14630.0
26	17085.0
27	20768.0
28	25666.0
29	31571.0
30	39194.0
31	48298.0
32	59135.0
33	78640.0
34	190497.0
35	177432.0
36	175160.0
37	333287.0
38	491383.0
39	213158.0
40	348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.019089752976235	22.96646335879261	30.25114240016158	13.763304488069586
2	30.859085911561323	25.582239198371955	30.773050098287747	12.785624791778977
3	29.58681523437829	24.63955796007008	33.000476711669634	12.773150093882002
4	26.829857496377635	26.934060826032912	31.823300854566313	14.412780823023144
5	24.60223287191802	31.852755002378608	29.432317090979655	14.112695034723718
6	22.303175751167398	40.74553150876026	26.447547608050932	10.503745132021411
7	88.24715148688993	3.732360306897369	6.390906539266339	1.6295816669463572
8	89.21136642989492	3.1995619994960616	6.03711024613272	1.551961324476298
9	84.42618122270851	6.041367484303909	7.251264672002075	2.281186620985511
10-11	36.63803921510389	29.27697740050067	21.23411394250451	12.850869441890927
12-13	28.376398267601083	25.074489292303458	28.83365534884849	17.715457091246968
14-15	18.10576167683701	30.67857406282595	33.81346069405853	17.402203566278516
16-17	23.13026802284454	26.872454629474245	31.158924186013586	18.83835316166763
18-19	24.19155789670653	28.79660252592831	29.01617205974588	17.995667517619275
20-21	26.389258097044237	27.809096529905364	29.06082355777394	16.740821815276455
22-23	22.61291457950615	24.14287192296973	32.096481887679204	21.147731609844914
24-25	20.609116727035293	27.876890572643088	32.62541897906478	18.888573721256837
26-27	19.340806271406855	32.6309385378565	28.938997242200713	19.08925794853593
28-29	18.247809873723387	28.496764251476048	34.015011219802695	19.240414654997874
30-31	22.829588201311925	27.903894333368477	30.32180760352638	18.944709861793218
32-33	24.92531269661881	27.88577631975621	28.180367300648633	19.008543682976345
34-35	20.97897666884974	27.847807695601528	30.263320081441957	20.909895554106775
36-37	22.48712804237833	29.935908764415824	29.164556611119608	18.412406582086234
38-39	20.98976827258601	30.38462661793614	27.960698761292203	20.66490634818565
40-41	21.16287945729124	27.041432332931866	29.493106491842685	22.30258171793421
42-43	21.216169188585255	27.434756587457287	28.407362249881068	22.94171197407639
44-45	20.321307625555114	30.04649795132789	28.367388763564378	21.264805659552625
46-47	21.31856566715625	28.988202995016554	28.8376403217878	20.855591016039394
48-49	21.243915490852135	27.383001442015676	30.70020677306792	20.67287629406427
50-51	20.08374383504885	29.560776777656827	29.433950682370924	20.921528704923396
52-53	21.789138993380984	27.02249752362396	30.08540712810178	21.102956354893273
54-55	21.539125256362468	27.50195659696182	30.37638440682564	20.582533739850074
56-57	22.15545948718101	26.777038041393226	29.171412744686005	21.896089726739763
58-59	22.524873904070564	28.29603052142752	28.304396489461602	20.874699085040312
60-61	23.25060925533479	28.958600338895963	27.243378880831486	20.54741152493776
62-63	22.988888113345503	27.091850903598804	29.490928369987657	20.428332613068036
64-65	21.23411394250451	29.53855003418166	29.408828026883967	19.81850799642986
66-67	19.79402887694552	29.568004181993963	27.0810097970931	23.55695714396742
68-69	19.44201468351147	31.124990037567652	27.029898187654105	22.403097091266773
70-71	20.279997564463745	29.373829568895232	28.8084831905921	21.537689676048927
72-73	22.3683461471252	29.707032709944958	27.499827977876222	20.42479316505362
74-75	20.941428818235234	30.863318398347793	26.662364876002986	21.53288790741398
76-77	23.460327738035307	28.127820110896106	27.37282862289731	21.03902352817128
78-79	20.093025604317432	29.22581628829225	29.048002340490935	21.633155766899378
80-81	18.914018144745107	29.15336898522788	28.931398567092835	23.001214302934176
82-83	19.995183380534222	28.64151034929649	29.508526604520892	21.854779665648394
84-85	19.063764022278225	27.983940311540728	28.865163861592237	24.087131804588807
86	19.771752630700927	28.254151178487678	29.250938943779207	22.723157247032187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	649.0
1	894.0
2	3547.5
3	4001.0
4	2176.0
5	2527.0
6	2972.0
7	3196.0
8	3293.0
9	3470.0
10	3486.5
11	3475.0
12	3590.0
13	3726.0
14	3799.0
15	3842.5
16	3931.0
17	4293.5
18	4543.0
19	4871.5
20	5504.0
21	5740.0
22	6422.5
23	7729.0
24	9008.0
25	12488.5
26	15514.0
27	18078.0
28	21509.5
29	22577.0
30	25897.0
31	31373.0
32	35434.5
33	41162.0
34	48333.5
35	51897.0
36	52111.0
37	55157.0
38	62076.5
39	67734.0
40	75626.5
41	85329.0
42	92870.5
43	96818.0
44	100723.5
45	106694.5
46	107035.0
47	109132.5
48	113784.5
49	106784.0
50	98954.0
51	87758.0
52	74337.0
53	68382.5
54	60980.0
55	53364.5
56	46466.5
57	43511.0
58	39608.0
59	30761.0
60	22597.5
61	17570.0
62	13460.5
63	9854.5
64	8550.0
65	7452.5
66	5054.5
67	3068.0
68	2183.0
69	1738.5
70	1181.0
71	869.0
72	739.0
73	454.0
74	295.0
75	230.0
76	136.0
77	68.0
78	29.0
79	25.0
80	21.5
81	13.0
82	7.0
83	4.5
84	2.5
85	2.5
86	3.0
87	3.0
88	2.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	2020089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.968884174779273
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.07035074687766	15.188452091514733
2	7.457259942843761	2.8291180023404885
3	2.2993680052127217	1.3084933609822014
4	1.1714331778076974	0.8888312107331131
5	0.7360517271338642	0.6981039979324254
6	0.5263563735243237	0.5990635850423852
7	0.38931692133656454	0.5169435311680463
8	0.32872054291209907	0.4988369523496134
9	0.2745397332669248	0.4686941161543584
>10	3.814417124653409	17.815489011000892
>50	1.5791783475163121	21.62757946635843
>100	1.3480166483586755	36.3216367239322
>500	0.003940033070566245	0.4937194325242109
>1k	7.880066141132489E-4	0.2011449539913452
>5k	0.0	0.0
>10k+	2.6266887137108295E-4	0.5438935639756066
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10916	0.5403722311244702	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02301878778608269	0.0
2	0.0	0.0	0.0	0.08737238804824936	0.0
3	0.0	0.0	0.0	0.12742012851909	0.0
4	0.0	0.0	0.0	0.20731759838304153	0.0
5	0.0	0.0	0.0	0.3935470169878654	0.0
6	0.0	0.0	0.0	0.5526984207131468	0.0
7	0.0	0.0	0.0	0.6781384384549394	0.0
8	0.0	0.0	0.0	0.8828818928274942	0.0
9	0.0	0.0	0.0	0.9532253281909856	0.0
10	0.0	0.0	0.0	1.1146538593101591	0.0
11	0.0	0.0	0.0	1.3420695820827697	0.0
12	0.0	0.0	0.0	1.563099447598596	0.0
13	0.0	0.0	0.0	1.6277500644773573	0.0
14	0.0	0.0	0.0	1.6622039920023326	0.0
15	0.0	0.0	0.0	1.7094296340408763	0.0
16	0.0	0.0	0.0	1.8143260024682082	0.0
17	0.0	0.0	0.0	1.951844695951515	0.0
18	0.0	0.0	0.0	2.1147583101536616	0.0
19	0.0	0.0	0.0	2.2232188779801287	0.0
20	0.0	0.0	0.0	2.3340555787393527	0.0
21	0.0	0.0	0.0	2.485385544894309	0.0
22	0.0	0.0	0.0	2.656962143747132	0.0
23	4.95027694324359E-5	0.0	0.0	2.835172113703901	0.0
24	4.95027694324359E-5	0.0	0.0	2.9690771050186404	0.0
25	4.95027694324359E-5	0.0	0.0	3.083676016254729	0.0
26	4.95027694324359E-5	0.0	0.0	3.197037358255008	0.0
27	4.95027694324359E-5	0.0	0.0	3.323170414768854	0.0
28	4.95027694324359E-5	0.0	0.0	3.45761993654735	0.0
29	4.95027694324359E-5	0.0	0.0	3.61409819072328	0.0
30	4.95027694324359E-5	0.0	0.0	3.7756257273813185	0.0
31	4.95027694324359E-5	0.0	0.0	3.9358661920341134	0.0
32	4.95027694324359E-5	0.0	0.0	4.084077483714826	0.0
33	4.95027694324359E-5	0.0	0.0	4.2340213723256745	0.0
34	4.95027694324359E-5	0.0	0.0	4.389856090498983	0.0
35	4.95027694324359E-5	0.0	0.0	4.565838435831292	0.0
36	4.95027694324359E-5	0.0	0.0	4.725583872789763	0.0
37	4.95027694324359E-5	0.0	0.0	4.90131870427491	0.0
38	4.95027694324359E-5	0.0	0.0	5.089132211501572	0.0
39	4.95027694324359E-5	0.0	0.0	5.34778418178605	0.0
40	9.90055388648718E-5	0.0	0.0	5.547478353676496	0.0
41	9.90055388648718E-5	0.0	0.0	5.746875508950348	0.0
42	9.90055388648718E-5	0.0	0.0	5.938649237731605	0.0
43	9.90055388648718E-5	0.0	0.0	6.118492799079645	0.0
44	9.90055388648718E-5	0.0	0.0	6.301554040440792	0.0
45	9.90055388648718E-5	0.0	0.0	6.4831797014884	0.0
46	9.90055388648718E-5	0.0	0.0	6.679656193365738	0.0
47	9.90055388648718E-5	0.0	0.0	6.8848451726631845	0.0
48	9.90055388648718E-5	0.0	0.0	7.088153046722199	0.0
49	1.4850830829730768E-4	0.0	0.0	7.299876391584728	0.0
50	1.4850830829730768E-4	0.0	0.0	7.515262941385256	0.0
51	1.4850830829730768E-4	0.0	0.0	7.74173811153865	0.0
52	1.4850830829730768E-4	0.0	0.0	7.9807869851278825	0.0
53	1.4850830829730768E-4	0.0	0.0	8.200678286946763	0.0
54	1.4850830829730768E-4	0.0	0.0	8.42556936847832	0.0
55	1.4850830829730768E-4	0.0	0.0	8.648876361388037	0.0
56	1.4850830829730768E-4	0.0	0.0	8.881687886028784	0.0
57	1.4850830829730768E-4	0.0	0.0	9.115934990983071	0.0
58	1.4850830829730768E-4	0.0	0.0	9.335133254029897	0.0
59	1.4850830829730768E-4	0.0	0.0	9.571063453144886	0.0
60	1.4850830829730768E-4	0.0	0.0	9.805756083024065	0.0
61	1.4850830829730768E-4	0.0	0.0	10.054061974497163	0.0
62	1.4850830829730768E-4	0.0	0.0	10.333455605173832	0.0
63	1.980110777297436E-4	0.0	0.0	10.615027357705527	0.0
64	1.980110777297436E-4	0.0	0.0	10.879421649244167	0.0
65	1.980110777297436E-4	0.0	0.0	11.124509860704157	0.0
66	1.980110777297436E-4	0.0	0.0	11.367370447539688	0.0
67	2.475138471621795E-4	0.0	0.0	11.62815103690976	0.0
68	2.9701661659461536E-4	0.0	0.0	11.900762788174184	0.0
69	2.9701661659461536E-4	0.0	0.0	12.249064273900803	0.0
70	3.465193860270513E-4	0.0	0.0	12.517517792532903	0.0
71	3.465193860270513E-4	0.0	0.0	12.785129764084651	0.0
72	3.960221554594872E-4	0.0	0.0	13.04808847530975	0.0
73	4.4552492489192307E-4	0.0	0.0	13.312136247462364	0.0
74	4.4552492489192307E-4	0.0	0.0	13.597222696623763	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	7140	0.0	71.596634	1
TACGGGA	890	0.0	68.31461	4
GGGCGAT	1640	0.0	64.39024	7
TAGCGGG	1355	0.0	64.35424	3
TCGATAG	145	0.0	63.44828	1
ACGGGAT	1310	0.0	62.290073	5
TAGGGAC	2385	0.0	62.05451	5
CCGATCC	1445	0.0	62.006916	80
CTAGCGG	175	0.0	61.714287	2
CGAGGGA	1695	0.0	61.35693	4
CGGGATT	1180	0.0	61.01695	6
TAATGCG	105	0.0	60.952385	1
CGTTAGG	375	0.0	60.8	2
GTAGGGT	1340	0.0	59.999996	4
CGAAGGG	1185	0.0	59.746838	3
TATGGGA	2710	0.0	59.630997	4
TAAGGGA	2950	0.0	59.525425	4
CGAGGGT	370	0.0	59.45946	4
GCGAGGG	2100	0.0	59.42857	3
TAGGGTA	1430	0.0	59.020977	5
>>END_MODULE