FastQCFastQC Report
Thu 26 May 2016
SRR1547840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547840_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1911647
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287631.5046187920677825No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTC25020.13088190445202488No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT23020.12041972184195095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT130500.075.5862051
ACGGGTA2050.066.341465
TAACGGG4050.065.185193
CCGATCC19300.064.45595680
GGGCGAT13250.064.07
TACGGGA6100.062.950824
GGCGATA3750.062.9333348
ACGAGGG9600.062.4999963
CGAGGGA10700.062.0560764
TAGCGCG650.061.5384641
AGGGTAA14450.061.453296
TAGGGAC16750.061.3731355
TAGGGTA16000.061.255
TACGCGG1200.059.9999962
GTAGGGT17750.059.718314
CGAGGGC5700.059.649124
CGGGTAT2550.059.6078386
CGTAAGG3300.059.393942
GGCGATT4650.059.354848
GTAGGGA24650.058.9046674