##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547840_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1911647
Sequences flagged as poor quality	0
Sequence length	86
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.180527576482476	33.0	31.0	34.0	31.0	34.0
2	32.62401845110525	34.0	31.0	34.0	31.0	34.0
3	32.69816760102676	34.0	31.0	34.0	31.0	34.0
4	36.25728442541955	37.0	37.0	37.0	35.0	37.0
5	36.245746207328025	37.0	37.0	37.0	35.0	37.0
6	36.294890217702324	37.0	37.0	37.0	35.0	37.0
7	36.424973857621204	37.0	37.0	37.0	35.0	37.0
8	36.40841693053163	37.0	37.0	37.0	35.0	37.0
9	38.19394741811642	39.0	39.0	39.0	37.0	39.0
10-11	37.91360931176101	39.0	38.0	39.0	35.0	39.0
12-13	37.4586322684052	39.0	37.0	39.0	35.0	39.0
14-15	38.713594873949006	40.0	38.0	41.0	35.0	41.0
16-17	38.798722776746956	40.0	38.0	41.0	35.0	41.0
18-19	38.553187905507656	40.0	37.5	41.0	35.0	41.0
20-21	38.31083301467268	40.0	35.5	41.0	35.0	41.0
22-23	38.14084294851507	40.0	35.0	41.0	34.0	41.0
24-25	38.10681914600342	40.0	35.0	41.0	34.0	41.0
26-27	38.10477091220294	40.0	35.0	41.0	34.5	41.0
28-29	37.980330050474805	40.0	35.0	41.0	34.0	41.0
30-31	37.75724623845302	40.0	35.0	41.0	34.0	41.0
32-33	37.307666373551186	40.0	35.0	41.0	33.0	41.0
34-35	36.8145031483323	40.0	35.0	41.0	31.5	41.0
36-37	36.596479894039014	40.0	35.0	41.0	31.0	41.0
38-39	36.487801618185784	40.0	35.0	41.0	31.0	41.0
40-41	36.35654647536914	40.0	35.0	41.0	30.0	41.0
42-43	36.28916609604179	40.0	35.0	41.0	30.0	41.0
44-45	36.13290816766903	39.0	35.0	41.0	30.0	41.0
46-47	35.99635471402408	39.0	35.0	41.0	29.0	41.0
48-49	35.86435414069648	39.0	35.0	41.0	29.0	41.0
50-51	35.644534006539914	38.5	35.0	40.5	28.5	41.0
52-53	35.44521085744387	38.0	35.0	40.0	28.0	41.0
54-55	35.23408741258193	37.5	35.0	40.0	27.5	41.0
56-57	34.93010425041862	37.0	35.0	40.0	26.5	41.0
58-59	34.655194709065015	36.5	34.5	40.0	26.0	41.0
60-61	34.27311318459945	36.0	34.0	39.5	25.5	41.0
62-63	33.89230961573972	35.5	34.0	39.0	24.5	40.5
64-65	33.46752721606029	35.0	34.0	38.5	22.5	40.0
66-67	33.05310473115591	35.0	33.5	38.0	20.5	40.0
68-69	32.46039854638435	35.0	33.0	37.0	18.5	39.5
70-71	32.088498294925785	35.0	33.0	36.5	16.5	39.0
72-73	31.789048396487424	35.0	33.0	36.0	15.0	39.0
74-75	31.475578127133303	35.0	32.5	36.0	12.5	37.5
76-77	29.660211064071973	33.0	29.5	34.5	10.5	36.0
78-79	30.64617186122752	34.0	31.0	35.0	11.0	36.0
80-81	30.69611204369844	35.0	32.0	35.0	10.0	36.0
82-83	30.481593882134096	35.0	31.5	35.0	10.0	36.0
84-85	30.23633024297896	34.0	31.0	35.0	10.0	35.5
86	29.83885937100312	34.0	30.0	35.0	10.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	11.0
10	18.0
11	25.0
12	39.0
13	82.0
14	193.0
15	607.0
16	1814.0
17	4877.0
18	12783.0
19	21876.0
20	25607.0
21	24710.0
22	20410.0
23	15508.0
24	12964.0
25	12591.0
26	13887.0
27	16304.0
28	20392.0
29	25855.0
30	33989.0
31	45083.0
32	57372.0
33	73551.0
34	171000.0
35	165841.0
36	175249.0
37	332529.0
38	449621.0
39	176504.0
40	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.081052098007632	23.09197252421603	28.505471983059632	17.321503394716704
2	34.18356004011201	24.387138420430134	28.491504969275184	12.937796570182675
3	28.024473137561483	24.172506744184467	35.36552512048511	12.43749499776894
4	25.903317924282042	25.59703752837213	34.03018444304832	14.469460104297498
5	23.62141127519882	29.598037713029658	32.493708304932866	14.286842706838657
6	21.370681930293618	39.419411638236554	28.971666840164527	10.238239591305298
7	83.0875679453372	4.033276018009601	10.558905488304065	2.3202505483491462
8	83.14662696617106	3.6828975223982248	10.754077504894994	2.416398006535726
9	78.45747672033592	5.935666992912394	12.141415229903847	3.4654410568478387
10-11	34.91442196179525	25.728834873802537	24.36671100888396	14.990032155518252
12-13	28.41416328433021	22.44616814715269	30.28485384592448	18.854814722592614
14-15	18.540452290616415	28.450257814334968	34.31172177708541	18.6975681179632
16-17	23.65172544931151	25.55597346162759	32.622183907384574	18.170117181676325
18-19	24.138138474310374	28.23248748330628	29.9619908905776	17.667383151805748
20-21	26.016178719188222	26.99967619544822	30.519049803650987	16.465095281712575
22-23	22.65985822696345	24.123622195938896	32.96976376914776	20.2467558079499
24-25	21.748759054365163	26.68672615812438	32.46417879451594	19.100335992994523
26-27	19.713629137597056	30.356807506825266	30.292491239229836	19.637072116347838
28-29	18.90848571938229	27.874157728911246	33.534303142787344	19.683053408919115
30-31	22.372252827012517	26.649297699836843	31.58765713544394	19.3907923377067
32-33	24.427417823478915	26.562749294195005	29.71116006250108	19.298672819825
34-35	21.176739220159373	27.194822056582623	30.576408719810715	21.05203000344729
36-37	22.192852550706277	28.928118005050095	29.40467042293896	19.474359021304664
38-39	21.295615770066338	28.8403664484081	28.414032507047587	21.449985274477974
40-41	20.92985786601815	26.771339059983358	29.544314405326926	22.75448866867157
42-43	20.652165384090264	27.423996166656288	29.104196538377643	22.819641910875806
44-45	21.39772667234066	28.324476223905354	28.386124634935218	21.891672468818772
46-47	21.218169463295265	28.41416328433021	28.826660989188902	21.54100626318562
48-49	20.86760787948821	27.091821868786443	30.416598880441835	21.623971371283506
50-51	19.409022690904752	27.756850506395793	30.280172019206475	22.553954783492976
52-53	21.028437781661573	25.743952727674095	30.833752256562015	22.39385723410232
54-55	20.606079469692887	25.827964054032986	31.195639153044468	22.370317323229656
56-57	21.63056254632785	26.57287145587025	29.395620634981253	22.400945362820647
58-59	22.119408028783557	27.987044679273946	28.885092279066164	21.008455012876333
60-61	22.878910175361874	28.522028387040077	28.116488033617088	20.482573403980968
62-63	22.231117983602623	27.415547954198654	29.43678932355189	20.91654473864683
64-65	21.246443511798986	30.10176565024819	28.164457140884274	20.48733369706855
66-67	20.42578990786479	30.200816364109063	26.616054114593336	22.757339613432816
68-69	19.571421920469625	31.088323314921634	27.303184112966463	22.037070651642274
70-71	19.783987315649803	29.850071692106333	28.859302998932336	21.506637993311525
72-73	20.8378952808756	30.786410880251424	27.663423215687832	20.712270623185137
74-75	19.646540391609957	30.9413296492501	27.01986820788566	22.392261751254285
76-77	21.275737623107194	29.035015355868527	27.439349419636578	22.2498976013877
78-79	19.243955604774314	29.54679917369682	27.822186836795705	23.387058384733166
80-81	18.45793182527946	29.025337836954208	28.359419913823004	24.157310423943333
82-83	19.8470219658755	29.523808527411177	28.081230478221137	22.547939028492184
84-85	18.636050484215964	28.705953557325177	28.11112616502942	24.546869793429433
86	18.90453624544699	29.286683158553856	28.26447560663658	23.544304989362576
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1008.0
1	1188.5
2	8345.5
3	9744.0
4	4446.0
5	5315.0
6	6449.0
7	6994.0
8	7268.5
9	7448.0
10	7144.0
11	6969.5
12	7028.0
13	7120.0
14	7188.0
15	7320.5
16	7498.5
17	7434.0
18	7437.0
19	7720.5
20	7963.0
21	8035.0
22	8292.0
23	8799.5
24	9873.5
25	11413.0
26	12558.0
27	13749.0
28	15459.5
29	16408.0
30	18185.0
31	22890.0
32	26887.0
33	29585.5
34	32453.0
35	37302.5
36	40914.0
37	42576.5
38	48637.5
39	57602.5
40	64501.0
41	72238.0
42	78136.5
43	78630.0
44	83126.0
45	87553.0
46	89349.0
47	92171.5
48	96427.5
49	97457.0
50	95188.0
51	88934.5
52	78150.0
53	72246.0
54	69078.0
55	64058.0
56	56240.0
57	51647.0
58	47733.0
59	38278.5
60	29323.0
61	23246.5
62	18991.5
63	14827.5
64	12257.0
65	10234.0
66	6591.0
67	4293.0
68	2843.5
69	1693.0
70	1159.5
71	1005.0
72	802.5
73	478.5
74	308.5
75	217.0
76	125.5
77	58.0
78	41.5
79	44.0
80	46.5
81	32.0
82	13.0
83	10.5
84	8.5
85	6.5
86	6.0
87	3.5
88	1.0
89	1.5
90	1.5
91	1.5
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1911647.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.388064467326757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.46393176371336	17.342674982186153
2	7.790654402095476	3.48835345993553
3	2.8426452529582757	1.9092397554291076
4	1.582918083491088	1.417538883987832
5	0.9926396138640257	1.1111639834005074
6	0.7481161121276212	1.0049323048415422
7	0.578275386072665	0.9062526636284167
8	0.46145816658133754	0.8264924145917917
9	0.3917597784383501	0.7893668857845086
>10	5.023012076604697	27.0711768941197
>50	1.5600138687494143	24.743585521413067
>100	0.5579887814334241	16.54838529562629
>500	0.00446955584058094	0.651842550058805
>1k	0.0018819182486656588	0.6741727788076887
>5k	0.0	0.0
>10k+	2.3523978108320735E-4	1.5148216261890124
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28763	1.5046187920677825	No Hit
GCCCTGCTAGAGGACTTGCGTCTGTATCTATGCTCTGCCCCATGCAGCTC	2502	0.13088190445202488	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2302	0.12041972184195095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0462182610073932E-4	0.0	0.0	0.0420579740924972	0.0
2	1.0462182610073932E-4	0.0	0.0	0.12664472049494493	0.0
3	1.0462182610073932E-4	0.0	0.0	0.19182411815570552	0.0
4	1.0462182610073932E-4	0.0	0.0	0.30549573221415877	0.0
5	1.0462182610073932E-4	0.0	0.0	0.6297710822134003	0.0
6	1.0462182610073932E-4	0.0	0.0	1.0713274992715704	0.0
7	1.0462182610073932E-4	0.0	0.0	1.370545921919685	0.0
8	1.0462182610073932E-4	0.0	0.0	1.8493477090697183	0.0
9	1.0462182610073932E-4	0.0	0.0	2.1364823108031974	0.0
10	1.0462182610073932E-4	0.0	0.0	2.531377393420438	0.0
11	1.0462182610073932E-4	0.0	0.0	2.9720445249567518	0.0
12	1.0462182610073932E-4	0.0	0.0	3.3051081083484557	0.0
13	1.0462182610073932E-4	0.0	0.0	3.452206395846095	0.0
14	1.0462182610073932E-4	0.0	0.0	3.532974445595866	0.0
15	1.0462182610073932E-4	0.0	0.0	3.6147364026935938	0.0
16	1.0462182610073932E-4	0.0	0.0	3.7637178830610463	0.0
17	1.0462182610073932E-4	0.0	0.0	3.9391163745189357	0.0
18	1.0462182610073932E-4	0.0	0.0	4.147836917589911	0.0
19	1.0462182610073932E-4	0.0	0.0	4.291011886608772	0.0
20	2.0924365220147863E-4	0.0	0.0	4.444335172759406	0.0
21	2.0924365220147863E-4	0.0	0.0	4.637937861958824	0.0
22	2.0924365220147863E-4	0.0	0.0	4.851732563595685	0.0
23	2.0924365220147863E-4	0.0	0.0	5.049886302230485	0.0
24	2.0924365220147863E-4	0.0	0.0	5.206609797729392	0.0
25	2.0924365220147863E-4	0.0	0.0	5.353760396140082	0.0
26	2.6155456525184825E-4	0.0	0.0	5.490344190114597	0.0
27	2.6155456525184825E-4	0.0	0.0	5.64774772748316	0.0
28	2.6155456525184825E-4	0.0	0.0	5.808917650591349	0.0
29	2.6155456525184825E-4	0.0	0.0	5.985728536701598	0.0
30	2.6155456525184825E-4	0.0	0.0	6.191258114076501	0.0
31	2.6155456525184825E-4	0.0	0.0	6.371469209535024	0.0
32	2.6155456525184825E-4	0.0	0.0	6.541008878731272	0.0
33	2.6155456525184825E-4	0.0	0.0	6.715675017406456	0.0
34	2.6155456525184825E-4	0.0	0.0	6.887045568559468	0.0
35	2.6155456525184825E-4	0.0	0.0	7.086873256411879	0.0
36	2.6155456525184825E-4	0.0	0.0	7.27650031621947	0.0
37	2.6155456525184825E-4	0.0	0.0	7.479518969767954	0.0
38	2.6155456525184825E-4	0.0	0.0	7.711465558233293	0.0
39	2.6155456525184825E-4	0.0	0.0	8.046726199973111	0.0
40	2.6155456525184825E-4	0.0	0.0	8.28709484543956	0.0
41	2.6155456525184825E-4	0.0	0.0	8.531909918515291	0.0
42	2.6155456525184825E-4	0.0	0.0	8.764118061545881	0.0
43	2.6155456525184825E-4	0.0	0.0	8.963265707528638	0.0
44	2.6155456525184825E-4	0.0	0.0	9.15446209472774	0.0
45	2.6155456525184825E-4	0.0	0.0	9.341787474361114	0.0
46	2.6155456525184825E-4	0.0	0.0	9.559662427215903	0.0
47	2.6155456525184825E-4	0.0	0.0	9.788156495419917	0.0
48	3.138654783022179E-4	0.0	0.0	10.00352052444829	0.0
49	3.661763913525876E-4	0.0	0.0	10.22008770447682	0.0
50	3.661763913525876E-4	0.0	0.0	10.426245012808327	0.0
51	3.661763913525876E-4	0.0	0.0	10.646526267663434	0.0
52	3.661763913525876E-4	0.0	0.0	10.900914237827381	0.0
53	3.661763913525876E-4	0.0	0.0	11.125275743900417	0.0
54	3.661763913525876E-4	0.0	0.0	11.344039982277062	0.0
55	3.661763913525876E-4	0.0	0.0	11.55720695295732	0.0
56	3.661763913525876E-4	0.0	0.0	11.773146402029244	5.231091305036966E-5
57	3.661763913525876E-4	0.0	0.0	11.99180601857979	5.231091305036966E-5
58	3.661763913525876E-4	0.0	0.0	12.220404708609905	5.231091305036966E-5
59	3.661763913525876E-4	0.0	0.0	12.449997305987978	5.231091305036966E-5
60	3.661763913525876E-4	0.0	0.0	12.691673724280685	5.231091305036966E-5
61	3.661763913525876E-4	0.0	0.0	12.953646776836937	5.231091305036966E-5
62	3.661763913525876E-4	0.0	0.0	13.27520195935756	5.231091305036966E-5
63	3.661763913525876E-4	0.0	0.0	13.563644333917297	5.231091305036966E-5
64	3.661763913525876E-4	0.0	0.0	13.83011612499588	5.231091305036966E-5
65	3.661763913525876E-4	0.0	0.0	14.07064170320148	5.231091305036966E-5
66	3.661763913525876E-4	0.0	0.0	14.32377421145222	5.231091305036966E-5
67	3.661763913525876E-4	0.0	0.0	14.56314894957071	5.231091305036966E-5
68	4.1848730440295726E-4	0.0	0.0	14.851015904086895	5.231091305036966E-5
69	4.1848730440295726E-4	0.0	0.0	15.19998200504591	5.231091305036966E-5
70	4.1848730440295726E-4	0.0	0.0	15.480786986300295	5.231091305036966E-5
71	4.1848730440295726E-4	0.0	0.0	15.750606675814101	5.231091305036966E-5
72	4.1848730440295726E-4	0.0	0.0	15.998246538194552	5.231091305036966E-5
73	4.1848730440295726E-4	0.0	0.0	16.256034717706772	5.231091305036966E-5
74	4.1848730440295726E-4	0.0	0.0	16.538042849961315	5.231091305036966E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	13050	0.0	75.586205	1
ACGGGTA	205	0.0	66.34146	5
TAACGGG	405	0.0	65.18519	3
CCGATCC	1930	0.0	64.455956	80
GGGCGAT	1325	0.0	64.0	7
TACGGGA	610	0.0	62.95082	4
GGCGATA	375	0.0	62.933334	8
ACGAGGG	960	0.0	62.499996	3
CGAGGGA	1070	0.0	62.056076	4
TAGCGCG	65	0.0	61.538464	1
AGGGTAA	1445	0.0	61.45329	6
TAGGGAC	1675	0.0	61.373135	5
TAGGGTA	1600	0.0	61.25	5
TACGCGG	120	0.0	59.999996	2
GTAGGGT	1775	0.0	59.71831	4
CGAGGGC	570	0.0	59.64912	4
CGGGTAT	255	0.0	59.607838	6
CGTAAGG	330	0.0	59.39394	2
GGCGATT	465	0.0	59.35484	8
GTAGGGA	2465	0.0	58.904667	4
>>END_MODULE