FastQCFastQC Report
Thu 26 May 2016
SRR1547839_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547839_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1194418
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66840.5596030870264849No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15800.1322819984293606No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT304.060348E-880.0000180
CGGGTAG404.1836756E-1180.06
TAGGTCG251.2848359E-680.01
AAGCGTA251.2848359E-680.01
TCGCGAG351.2896635E-980.01
CGTAAGG2050.078.048782
CTAGCGG1100.076.363632
GTATGCG750.074.6666641
CGTTTTT40650.073.702341
GTTAGCG600.073.3333361
CGCTAGG1600.072.52
CGGGACG1050.072.380966
CGGGTTG351.1815246E-768.571436
CTACGGG4350.068.0459753
GCGATAA1000.068.09
CGAGGGA8250.067.3939364
TAGCACG303.795676E-666.666671
TATCGTG303.795676E-666.666671
CGCGAGG900.066.6666642
GCTAGCG900.066.6666641
Produced by FastQC (version 0.11.5)