##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547839_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1194418
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.071110783662	33.0	31.0	34.0	31.0	34.0
2	32.505149788432526	34.0	31.0	34.0	31.0	34.0
3	32.60472548136415	34.0	31.0	34.0	31.0	34.0
4	36.120887327552	37.0	35.0	37.0	35.0	37.0
5	36.08543993811212	37.0	35.0	37.0	35.0	37.0
6	36.130450981147305	37.0	36.0	37.0	35.0	37.0
7	36.35016217103225	37.0	37.0	37.0	35.0	37.0
8	36.3607731966531	37.0	37.0	37.0	35.0	37.0
9	38.176942243000354	39.0	39.0	39.0	37.0	39.0
10-11	37.71339891059914	39.0	37.5	39.0	35.0	39.0
12-13	36.678082128702016	39.0	35.0	39.0	33.5	39.0
14-15	37.73889584718248	40.0	35.0	41.0	33.0	41.0
16-17	37.96886935729368	40.0	35.0	41.0	34.0	41.0
18-19	37.83522058441852	39.0	36.0	41.0	34.0	41.0
20-21	37.61263100522598	39.0	35.0	41.0	34.0	41.0
22-23	37.41047397142374	39.0	35.0	41.0	33.0	41.0
24-25	37.3939416519175	39.0	35.0	41.0	34.0	41.0
26-27	37.353696946964966	39.0	35.0	41.0	33.5	41.0
28-29	37.33587487797404	39.0	35.0	41.0	33.5	41.0
30-31	37.224879397329914	39.0	35.0	41.0	33.5	41.0
32-33	36.856791759668724	39.0	35.0	41.0	32.5	41.0
34-35	36.570274393051676	39.0	35.0	41.0	32.0	41.0
36-37	36.37360706218426	38.0	35.0	41.0	31.0	41.0
38-39	36.345059267358664	38.0	35.0	41.0	31.0	41.0
40-41	36.2389950586813	38.0	35.0	41.0	31.0	41.0
42-43	36.18396114258158	38.0	35.0	41.0	31.0	41.0
44-45	36.024655941219905	38.0	35.0	41.0	31.0	41.0
46-47	35.90084543267098	38.0	35.0	40.0	30.0	41.0
48-49	35.84093508302789	37.0	35.0	40.0	30.0	41.0
50-51	35.629686592131065	37.0	35.0	40.0	30.0	41.0
52-53	35.419429797608544	36.5	35.0	40.0	29.0	41.0
54-55	35.26122848115149	36.0	35.0	40.0	29.0	41.0
56-57	34.95307630996854	36.0	34.5	40.0	28.5	41.0
58-59	34.691152092483534	35.5	34.0	39.0	28.0	41.0
60-61	34.33959635571466	35.0	34.0	39.0	27.0	40.5
62-63	33.861218601863	35.0	33.5	38.5	26.0	40.0
64-65	33.566711988600304	35.0	33.0	38.0	26.0	40.0
66-67	33.320837010158925	35.0	33.0	37.0	25.5	40.0
68-69	33.00761919194118	35.0	33.5	36.5	25.0	39.0
70-71	32.77303464951131	35.0	33.0	36.0	24.5	39.0
72-73	32.56354015093544	35.0	33.0	36.0	24.0	38.5
74-75	32.26820049597377	35.0	33.0	35.0	24.0	37.5
76-77	30.31826839515145	33.0	30.0	34.5	19.5	35.5
78-79	31.27000221028149	34.0	31.5	35.0	20.0	36.0
80-81	31.644887719374623	35.0	32.5	35.0	21.0	36.0
82-83	31.542606106070068	35.0	32.5	35.0	20.0	36.0
84-85	31.374511268249474	35.0	32.0	35.0	20.0	36.0
86	30.995888374086793	34.0	31.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	4.0
11	6.0
12	8.0
13	33.0
14	138.0
15	419.0
16	1022.0
17	2535.0
18	4928.0
19	8207.0
20	10422.0
21	11167.0
22	9609.0
23	8163.0
24	7803.0
25	8684.0
26	10066.0
27	12174.0
28	15173.0
29	18648.0
30	23535.0
31	29863.0
32	37713.0
33	52073.0
34	160254.0
35	145609.0
36	94130.0
37	180739.0
38	240325.0
39	100597.0
40	364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.157616512812098	24.05631864221738	30.954657414740904	14.831407430229618
2	30.825054545393655	24.551455185705507	31.150987342789545	13.472502926111293
3	28.63788054098314	23.973098195104225	33.66895006605727	13.720071197855358
4	25.429037405665355	28.363855869553205	32.209661944143505	13.997444780637935
5	23.750060699018267	31.201388458646807	30.519968721167967	14.528582121166961
6	22.406393741554464	40.00015070101087	26.977155401207952	10.616300156226714
7	88.37191000135631	3.5109986621099147	6.633691052880984	1.4834002836527915
8	89.63763104708737	2.5814245934003	6.1422383118807655	1.6387060476315662
9	85.96714048180787	4.726234869199895	6.949577116218945	2.357047532773284
10-11	44.952730116257456	25.040019490664072	18.078218848007985	11.929031545070485
12-13	31.378336562242033	27.72316726640087	25.38483177581048	15.513664395546616
14-15	14.608872270846554	33.890145660899286	35.80735554889494	15.693626519359219
16-17	19.211783479485405	23.56465659425762	34.65583238028898	22.567727545967994
18-19	25.043326540624804	26.856050394418034	29.548993735861316	18.551629329095846
20-21	28.396717062201006	26.639166522942553	27.686287380129905	17.277829034726537
22-23	21.32243485948805	24.56309265265594	30.074772818226116	24.039699669629893
24-25	18.98782503277747	25.626623175471234	36.424894802322136	18.96065698942916
26-27	17.369421760221297	35.514283944146854	28.859076135825145	18.2572181598067
28-29	15.381424258509165	26.729670852247704	38.31803438997068	19.57087049927245
30-31	22.745429154617565	28.96653432885305	30.091349929421696	18.196686587107695
32-33	27.296599682858098	27.582847880725176	27.079715811382616	18.040836625034117
34-35	19.5393070097738	27.75987970710421	29.515002285631997	23.18581099748999
36-37	23.490394485012782	28.666764901399677	30.24146488080387	17.601375732783666
38-39	18.78220187572525	32.1991965961665	27.610769429127824	21.40783209898042
40-41	20.71531909264596	26.14980685153774	28.41053132153065	24.72434273428565
42-43	22.77477399034509	24.279565445262882	26.360327791443193	26.58533277294883
44-45	18.303809889000334	33.10997489990941	27.40443462841317	21.18178058267709
46-47	21.484689614523557	30.31656421788687	26.869362317044786	21.329383850544783
48-49	20.77233430842469	25.579445386790887	32.09094303669235	21.557277268092076
50-51	18.140717906126667	31.996168845412576	28.635033966333395	21.228079282127364
52-53	23.224365339437284	25.010088595449837	29.8545818967899	21.910964168322984
54-55	23.247514689162422	26.158514021054607	30.978476546736573	19.615494743046405
56-57	23.812727202704583	23.297455329708693	29.309211683012144	23.58060578457458
58-59	25.224209615059383	26.18551461883528	27.506702008844474	21.083573757260858
60-61	26.04352077748326	28.784269828485503	26.108447796332605	19.06376159769863
62-63	24.873536735045853	24.30292410194756	30.885167504173584	19.938371658833002
64-65	19.645007024341563	30.173607564520964	30.45265560297986	19.728729808157613
66-67	18.26228338822757	28.268663064354353	25.13445879080858	28.334594756609494
68-69	17.119802280273742	32.731882808196126	24.097342806287248	26.050972105242888
70-71	19.444825848237386	29.61078115031756	28.636080501131094	22.30831250031396
72-73	24.080388942564497	30.19106376494661	25.6853965697101	20.043150722778794
74-75	22.435445547538635	31.27615290459454	23.78375911950423	22.5046424283626
76-77	28.73889207965721	26.52417327937121	24.98827881026575	19.748655830705832
78-79	19.392038633041363	28.36573963218907	30.693819081761998	21.548402653007575
80-81	16.103491407530697	27.77796382840848	31.39909981262841	24.71944495143241
82-83	18.929051638538603	26.380337536775233	31.895157306738515	22.795453517947653
84-85	16.960938297982782	25.898596638697676	28.78133953105194	28.359125532267598
86	18.752564010254368	25.39219938078629	32.068337885062014	23.78689872389733
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	725.0
1	786.0
2	2474.0
3	2645.0
4	1307.0
5	1606.5
6	1996.0
7	2204.0
8	2293.0
9	2395.0
10	2349.0
11	2294.5
12	2318.5
13	2415.5
14	2493.0
15	2489.5
16	2636.5
17	2901.0
18	2816.0
19	2717.5
20	2866.5
21	2915.0
22	3030.0
23	3530.0
24	4048.0
25	5163.5
26	5895.0
27	6699.5
28	9787.5
29	11820.0
30	14112.0
31	15787.0
32	17824.5
33	19425.0
34	20139.0
35	25748.0
36	29589.0
37	29035.5
38	28301.0
39	31849.5
40	38767.0
41	48180.0
42	57758.5
43	61112.0
44	67371.0
45	71391.0
46	73025.0
47	80003.0
48	80410.0
49	68019.0
50	58326.0
51	51480.0
52	40209.5
53	35309.5
54	35450.5
55	34993.0
56	32093.5
57	30268.0
58	27402.0
59	20411.0
60	14010.5
61	10188.0
62	7719.0
63	5917.5
64	5038.0
65	4391.5
66	2983.5
67	1718.5
68	1136.0
69	831.5
70	461.5
71	317.0
72	273.5
73	161.5
74	85.5
75	66.0
76	40.5
77	36.5
78	32.0
79	18.0
80	17.0
81	12.0
82	5.5
83	4.0
84	3.5
85	2.5
86	3.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1194418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.919936017291356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.10656208324343	12.912112789477046
2	8.397511913839699	2.6737570474553976
3	2.541362543329114	1.2137498725962097
4	1.1179901413903328	0.7119332607558645
5	0.6332233374824088	0.504043750868782
6	0.4201422986098019	0.4013183107215459
7	0.2845132291093232	0.31706026824453676
8	0.2244608887985445	0.28587223904457426
9	0.19120547041137434	0.2739580969594657
>10	2.06256507028949	7.945468961853621
>50	1.0365270017408013	12.161411885604872
>100	1.9733267382168758	58.89275685717483
>500	0.007956962654100307	0.7404537207167894
>1k	0.0021218567077600815	0.40164209577201765
>5k	5.304641769400204E-4	0.5644608427544504
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6684	0.5596030870264849	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1580	0.1322819984293606	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.3722783816051E-5	0.0	0.0	0.03089370722812282	0.0
2	8.3722783816051E-5	0.0	0.0	0.12977031491487906	0.0
3	8.3722783816051E-5	0.0	0.0	0.2017719089966829	0.0
4	8.3722783816051E-5	0.0	0.0	0.3551520489476883	0.0
5	8.3722783816051E-5	0.0	0.0	0.6265813140793257	0.0
6	8.3722783816051E-5	0.0	0.0	0.8608376631966363	0.0
7	8.3722783816051E-5	0.0	0.0	0.9947941173023179	0.0
8	8.3722783816051E-5	0.0	0.0	1.2694048482189653	0.0
9	1.67445567632102E-4	0.0	0.0	1.349611275114742	0.0
10	1.67445567632102E-4	0.0	0.0	1.510442742825376	0.0
11	1.67445567632102E-4	0.0	0.0	1.7656297878966996	0.0
12	1.67445567632102E-4	0.0	0.0	1.9774484309513085	0.0
13	1.67445567632102E-4	0.0	0.0	2.047691846572975	0.0
14	1.67445567632102E-4	0.0	0.0	2.0754878107999044	0.0
15	1.67445567632102E-4	0.0	0.0	2.1224562925207087	0.0
16	1.67445567632102E-4	0.0	0.0	2.231630802616839	0.0
17	1.67445567632102E-4	0.0	0.0	2.36709426683121	0.0
18	1.67445567632102E-4	0.0	0.0	2.550949500091258	0.0
19	1.67445567632102E-4	0.0	0.0	2.653342464698288	0.0
20	1.67445567632102E-4	0.0	0.0	2.764693767173636	0.0
21	1.67445567632102E-4	0.0	0.0	2.9030875288215685	0.0
22	1.67445567632102E-4	0.0	0.0	3.048681449877681	0.0
23	1.67445567632102E-4	0.0	0.0	3.2023127581801347	0.0
24	1.67445567632102E-4	0.0	0.0	3.3196921010902383	0.0
25	1.67445567632102E-4	0.0	0.0	3.4221687884810845	0.0
26	1.67445567632102E-4	0.0	0.0	3.5160220291388775	0.0
27	1.67445567632102E-4	0.0	0.0	3.6148986368256337	0.0
28	1.67445567632102E-4	0.0	0.0	3.720221898866226	0.0
29	1.67445567632102E-4	0.0	0.0	3.835508172180928	0.0
30	1.67445567632102E-4	0.0	0.0	3.977753181884399	0.0
31	1.67445567632102E-4	0.0	0.0	4.110621239800472	0.0
32	1.67445567632102E-4	0.0	0.0	4.231600662414666	0.0
33	1.67445567632102E-4	0.0	0.0	4.353249867299388	0.0
34	1.67445567632102E-4	0.0	0.0	4.481010835402682	0.0
35	1.67445567632102E-4	0.0	0.0	4.649293630872944	0.0
36	2.51168351448153E-4	0.0	0.0	4.781408183734673	0.0
37	2.51168351448153E-4	0.0	0.0	4.924155530141039	0.0
38	2.51168351448153E-4	0.0	0.0	5.0731820853336105	0.0
39	2.51168351448153E-4	0.0	0.0	5.235185672017669	0.0
40	2.51168351448153E-4	0.0	0.0	5.400287001702922	0.0
41	2.51168351448153E-4	0.0	0.0	5.580039818555983	0.0
42	2.51168351448153E-4	0.0	0.0	5.741875959672409	0.0
43	2.51168351448153E-4	0.0	0.0	5.888139662999051	0.0
44	2.51168351448153E-4	0.0	0.0	6.0384220599488625	0.0
45	2.51168351448153E-4	0.0	0.0	6.194648774549614	0.0
46	2.51168351448153E-4	0.0	0.0	6.374317868618859	0.0
47	2.51168351448153E-4	0.0	0.0	6.553568348769024	0.0
48	2.51168351448153E-4	0.0	0.0	6.720092965779149	0.0
49	2.51168351448153E-4	0.0	0.0	6.904115644606829	0.0
50	2.51168351448153E-4	0.0	0.0	7.0849568576495	0.0
51	2.51168351448153E-4	0.0	0.0	7.2696493187477085	0.0
52	2.51168351448153E-4	0.0	0.0	7.46974677206807	0.0
53	2.51168351448153E-4	0.0	0.0	7.664737135575653	0.0
54	3.34891135264204E-4	0.0	0.0	7.86726254962668	0.0
55	3.34891135264204E-4	0.0	0.0	8.067694894082306	0.0
56	4.1861391908025496E-4	0.0	0.0	8.288555597789049	0.0
57	5.02336702896306E-4	0.0	0.0	8.492336853597317	0.0
58	5.02336702896306E-4	0.0	0.0	8.672926898288539	0.0
59	5.02336702896306E-4	0.0	0.0	8.870345222526787	0.0
60	5.02336702896306E-4	8.3722783816051E-5	0.0	9.073707864415974	0.0
61	5.02336702896306E-4	8.3722783816051E-5	0.0	9.297917479475359	0.0
62	5.02336702896306E-4	8.3722783816051E-5	0.0	9.549169553707328	0.0
63	5.02336702896306E-4	8.3722783816051E-5	0.0	9.815073115107106	0.0
64	5.02336702896306E-4	8.3722783816051E-5	0.0	10.076790537316082	0.0
65	5.02336702896306E-4	8.3722783816051E-5	0.0	10.2896975765603	0.0
66	5.02336702896306E-4	8.3722783816051E-5	0.0	10.495488179180153	0.0
67	5.02336702896306E-4	8.3722783816051E-5	0.0	10.71392092215623	0.0
68	5.02336702896306E-4	8.3722783816051E-5	0.0	10.956633272438962	0.0
69	5.02336702896306E-4	8.3722783816051E-5	0.0	11.223457784460717	0.0
70	5.02336702896306E-4	8.3722783816051E-5	0.0	11.449006964061159	0.0
71	5.02336702896306E-4	8.3722783816051E-5	0.0	11.675560817067392	0.0
72	5.02336702896306E-4	8.3722783816051E-5	0.0	11.912998631969712	0.0
73	5.02336702896306E-4	8.3722783816051E-5	0.0	12.151189951926378	0.0
74	5.02336702896306E-4	8.3722783816051E-5	0.0	12.42060987024643	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTGT	30	4.060348E-8	80.00001	80
CGGGTAG	40	4.1836756E-11	80.0	6
TAGGTCG	25	1.2848359E-6	80.0	1
AAGCGTA	25	1.2848359E-6	80.0	1
TCGCGAG	35	1.2896635E-9	80.0	1
CGTAAGG	205	0.0	78.04878	2
CTAGCGG	110	0.0	76.36363	2
GTATGCG	75	0.0	74.666664	1
CGTTTTT	4065	0.0	73.70234	1
GTTAGCG	60	0.0	73.333336	1
CGCTAGG	160	0.0	72.5	2
CGGGACG	105	0.0	72.38096	6
CGGGTTG	35	1.1815246E-7	68.57143	6
CTACGGG	435	0.0	68.045975	3
GCGATAA	100	0.0	68.0	9
CGAGGGA	825	0.0	67.393936	4
TAGCACG	30	3.795676E-6	66.66667	1
TATCGTG	30	3.795676E-6	66.66667	1
CGCGAGG	90	0.0	66.666664	2
GCTAGCG	90	0.0	66.666664	1
>>END_MODULE