FastQCFastQC Report
Thu 26 May 2016
SRR1547838_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547838_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1298728
Sequences flagged as poor quality0
Sequence length86
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76230.5869589321243555No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT16970.13066631350059443No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA16470.12681639265496703No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15320.11796157471002397No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT15290.1177305794592863No Hit
TCAGATAGGGCTGTCATCTCAGACTCAAAAAAGTGAATACCAGAACTCGC14470.11141670927245735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATCG150.001309829580.01
CTCGCGG304.06053E-880.02
CGTTTTT43050.071.8234561
ATTCGCG451.1823431E-1071.1111151
GCGTAAG1700.068.235291
TAGGGCG2600.067.692315
TAGGGTA12600.067.619055
CGAGGGA6350.066.771654
AGGGATT14200.066.760576
GTAGGGT12550.065.657374
TCGATTG1650.065.4545441
ATCGTAG559.094947E-1265.4545441
TCGATAG1350.065.185181
ACGGGTA1600.065.05
AGTAGGG27350.064.936023
CGTAGGG7600.064.736843
TTGTACG1550.064.516131
ATAGCCG251.2355868E-464.01
CGCGCGG503.0013325E-1064.02
CTTTACG251.2355868E-464.01
Produced by FastQC (version 0.11.5)