##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547838_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1298728
Sequences flagged as poor quality	0
Sequence length	86
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.968255862659465	33.0	31.0	34.0	30.0	34.0
2	32.38923700728713	33.0	31.0	34.0	31.0	34.0
3	32.50913817211918	34.0	31.0	34.0	31.0	34.0
4	36.05121472702521	37.0	35.0	37.0	35.0	37.0
5	35.99696934231032	37.0	35.0	37.0	35.0	37.0
6	36.05812687491145	37.0	35.0	37.0	35.0	37.0
7	36.299229707837206	37.0	36.0	37.0	35.0	37.0
8	36.31115137272778	37.0	37.0	37.0	35.0	37.0
9	38.15021775152303	39.0	39.0	39.0	37.0	39.0
10-11	37.62644449030128	39.0	37.5	39.0	35.0	39.0
12-13	36.77927017820514	39.0	35.0	39.0	33.5	39.0
14-15	37.7950575486168	40.0	35.0	41.0	33.0	41.0
16-17	38.036493014703616	40.0	35.0	41.0	34.0	41.0
18-19	37.94254955618113	40.0	36.0	41.0	34.0	41.0
20-21	37.73055404980873	40.0	35.0	41.0	33.5	41.0
22-23	37.54293970715962	39.5	35.0	41.0	33.0	41.0
24-25	37.52778295378247	39.0	35.0	41.0	33.0	41.0
26-27	37.50532482552159	39.0	35.0	41.0	33.0	41.0
28-29	37.47677573749084	39.0	35.0	41.0	33.0	41.0
30-31	37.36660216765944	39.0	35.0	41.0	33.0	41.0
32-33	37.053347968165774	39.0	35.0	41.0	32.5	41.0
34-35	36.78149466246974	39.0	35.0	41.0	32.0	41.0
36-37	36.59033107779304	39.0	35.0	41.0	31.0	41.0
38-39	36.512242363297005	39.0	35.0	41.0	31.0	41.0
40-41	36.39697765813935	39.0	35.0	41.0	31.0	41.0
42-43	36.36527471495186	39.0	35.0	41.0	31.0	41.0
44-45	36.22793263870494	38.5	35.0	41.0	30.5	41.0
46-47	36.10393785303774	38.0	35.0	41.0	30.0	41.0
48-49	36.046453529915425	38.0	35.0	40.0	30.0	41.0
50-51	35.836450357580645	38.0	35.0	40.0	30.0	41.0
52-53	35.65152402966595	37.0	35.0	40.0	29.5	41.0
54-55	35.45385138381555	37.0	35.0	40.0	29.0	41.0
56-57	35.11870961433033	36.5	34.5	40.0	28.5	41.0
58-59	34.83296733419161	36.0	34.0	39.5	28.0	41.0
60-61	34.44811769670015	35.5	34.0	39.0	27.5	40.5
62-63	33.953948786812944	35.0	33.5	39.0	26.0	40.0
64-65	33.52381753531148	35.0	33.0	38.0	25.5	40.0
66-67	33.223291944117626	35.0	33.0	37.0	24.5	40.0
68-69	32.75647633684652	35.0	33.0	37.0	23.0	39.5
70-71	32.45737521636555	35.0	33.0	36.0	21.5	39.0
72-73	32.17260812117703	35.0	33.0	36.0	20.0	38.5
74-75	31.89326094455498	35.0	32.5	35.0	20.0	37.5
76-77	29.983876531498513	33.0	29.5	34.5	18.0	35.5
78-79	30.92658970931558	34.0	31.0	35.0	18.0	36.0
80-81	31.144644991098986	34.5	32.0	35.0	18.0	36.0
82-83	30.948382571254335	34.5	31.5	35.0	17.5	36.0
84-85	30.749841768253244	34.0	31.0	35.0	15.0	35.5
86	30.390157908353405	34.0	31.0	35.0	15.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	6.0
12	14.0
13	37.0
14	140.0
15	438.0
16	1146.0
17	2407.0
18	5218.0
19	8379.0
20	10218.0
21	10692.0
22	9498.0
23	8680.0
24	9156.0
25	10445.0
26	12667.0
27	15251.0
28	19095.0
29	24016.0
30	30341.0
31	38127.0
32	45735.0
33	58105.0
34	151786.0
35	138635.0
36	107085.0
37	199448.0
38	271792.0
39	109938.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.31766466881441	24.360836141208935	31.65389519591477	13.667603994061883
2	29.056353601369956	25.25779069982321	32.323781423053944	13.362074275752892
3	28.240093383680033	24.879651474365687	33.993877085887114	12.886378056067166
4	25.926290955457954	26.571460690768202	32.84251975779378	14.659728595980068
5	22.843120345445698	31.97443960552171	31.17943095089965	14.003009098132942
6	20.816830005975078	40.86175088240186	27.82068300675738	10.500736104865684
7	88.26875219445488	3.260805957829507	6.508214191116231	1.9622276565993801
8	89.60967962498691	2.3321280514472624	6.216544187851498	1.8416481357143295
9	86.40608349092342	3.614151693041191	7.456526693811175	2.523238122224207
10-11	40.69832174250497	25.31985142385473	20.04934828539925	13.932478548241049
12-13	29.996042281370695	26.467012338226326	26.253033737626353	17.283911642776626
14-15	16.118078612303734	31.396835981052227	35.43701991487055	17.04806549177349
16-17	21.169906246727567	24.733508479065673	32.254559846249556	21.8420254279572
18-19	24.768581257969334	27.231067629249544	29.27341213864643	18.72693897413469
20-21	25.965252154415708	27.66899612543966	28.625855452411898	17.739896267732735
22-23	22.01534886442735	23.92795104132659	30.966568827344908	23.090131266901153
24-25	19.98590158986331	26.485992447995272	34.08265626058728	19.445449701554136
26-27	17.806923389655108	32.93226141270536	29.393722165072283	19.86709303256725
28-29	16.549924233557757	28.588665217043136	35.258768579718	19.602641969681102
30-31	22.75022175544071	27.576790521186883	30.684485126985788	18.98850259638662
32-33	25.518738334739837	27.73063335817816	27.249008260390166	19.50162004669184
34-35	19.541774721111736	27.42591212324675	29.98814994363716	23.044163212004364
36-37	22.785756524845848	27.358076517946788	30.255488447157525	19.600678510049832
38-39	19.594287641446094	29.76608650926137	28.351856585828592	22.287769263463943
40-41	20.876888771166865	25.198232424341356	29.071098798208713	24.85378000628307
42-43	21.346194122248846	25.586342944789052	28.18400003695924	24.883462896002857
44-45	20.128271662734615	29.98718746342575	27.485123905852493	22.399416967987136
46-47	21.52583142890582	28.068540910798873	28.522870069791367	21.88275759050394
48-49	20.92847771049827	26.06692856394873	31.196178106578127	21.808415618974873
50-51	18.918819029080762	29.325501567687766	29.317185738661212	22.438493664570256
52-53	22.595185442987294	24.451617274748834	30.482518279424177	22.470679002839702
54-55	22.376586937372565	25.674621629779292	30.33933972317529	21.60945170967285
56-57	22.281455393277113	23.460031661749035	30.572105937501924	23.686407007471928
58-59	23.68190260008254	25.657412483599334	28.707165780671552	21.95351913564657
60-61	24.18377828151853	28.482060908827716	26.729345944647378	20.604814865006375
62-63	23.64494335996452	25.779377975988815	30.007515045490663	20.568163618556
64-65	19.98262915714453	29.608971239551312	30.198047628140767	20.21035197516339
66-67	18.46283440412465	29.40669639832205	26.14046205210021	25.990007145453088
68-69	18.1415970087655	31.701287721524444	26.0514518821493	24.105663387560753
70-71	19.263386944764417	29.636575172014464	28.888689548542885	22.211348334678238
72-73	22.98949433599645	28.621851534732446	27.553152007194733	20.83550212207637
74-75	21.488102204618674	30.542076554906032	25.197038948879214	22.772782291596087
76-77	25.640395833461664	27.781529311757353	26.4149999076019	20.163074947179087
78-79	18.616561743490553	28.927111758582242	30.105380033386513	22.350946464540687
80-81	16.82681054077528	28.21610837681177	30.409485280982622	24.547595801430322
82-83	18.908578239631392	27.038417590134344	31.277642431671605	22.775361738562655
84-85	16.910161327083113	26.228971732341183	30.129673033922423	26.731193906653278
86	17.840533198637438	26.263621019951827	31.457934224872336	24.437911556538396
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	767.0
1	782.5
2	2655.0
3	2861.5
4	1314.0
5	1683.0
6	2098.0
7	2247.0
8	2356.5
9	2386.5
10	2303.5
11	2266.5
12	2277.5
13	2346.5
14	2371.0
15	2414.5
16	2505.0
17	2630.5
18	2889.5
19	2988.5
20	3229.5
21	3552.0
22	3371.0
23	3578.0
24	4520.0
25	5181.5
26	6005.5
27	7366.0
28	10236.0
29	12462.0
30	13948.5
31	16525.0
32	18400.5
33	20759.0
34	23246.5
35	25136.5
36	26112.0
37	28393.0
38	33928.0
39	39085.5
40	46767.0
41	58580.5
42	66114.5
43	67613.0
44	70196.0
45	72979.0
46	77782.5
47	83602.5
48	85170.0
49	80074.0
50	74627.0
51	62793.0
52	48857.5
53	41800.5
54	37598.0
55	36363.0
56	33028.0
57	31681.0
58	27942.5
59	20834.0
60	14649.0
61	9821.0
62	6772.5
63	4760.0
64	3783.0
65	3352.0
66	2320.5
67	1514.0
68	1067.0
69	645.0
70	464.5
71	465.0
72	320.0
73	167.5
74	119.0
75	58.0
76	38.5
77	28.0
78	27.5
79	38.0
80	25.0
81	11.5
82	9.5
83	7.0
84	3.5
85	1.5
86	2.0
87	2.5
88	1.5
89	1.0
90	1.0
91	1.5
92	1.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1298728.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.76926336556374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.79823905342782	14.789849015364373
2	8.89603914871005	3.339442033850088
3	2.90779231065229	1.637313590729819
4	1.409480990161448	1.058196796523831
5	0.8652622453584325	0.8120167481705727
6	0.5537502433984614	0.6236090494254448
7	0.4278282000835503	0.5621014113808279
8	0.3265433115606817	0.4903181931956617
9	0.2656369904272192	0.44872295696677916
>10	2.8050858987078144	12.540623554633187
>50	1.2337138101147755	16.985104339789924
>100	1.4982286636294693	44.29758466669341
>500	0.00950600255544215	1.1353760263061368
>1k	0.0024798267535936043	0.6883934720853141
>5k	4.133044589322674E-4	0.591348144884646
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7623	0.5869589321243555	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1697	0.13066631350059443	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA	1647	0.12681639265496703	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1532	0.11796157471002397	No Hit
GAGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	1529	0.1177305794592863	No Hit
TCAGATAGGGCTGTCATCTCAGACTCAAAAAAGTGAATACCAGAACTCGC	1447	0.11141670927245735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.699841691254828E-5	0.0	0.0	0.07615143432651025	0.0
2	7.699841691254828E-5	0.0	0.0	0.29051502701104465	0.0
3	7.699841691254828E-5	0.0	0.0	0.41694642758144895	0.0
4	7.699841691254828E-5	0.0	0.0	0.6844389279356417	0.0
5	7.699841691254828E-5	0.0	0.0	1.2305886990963466	0.0
6	7.699841691254828E-5	0.0	0.0	1.6258985715253695	0.0
7	7.699841691254828E-5	0.0	0.0	1.8916971067074861	0.0
8	7.699841691254828E-5	0.0	0.0	2.399193672578092	0.0
9	7.699841691254828E-5	0.0	0.0	2.5402547723618802	0.0
10	7.699841691254828E-5	0.0	0.0	2.906074251113397	0.0
11	7.699841691254828E-5	0.0	0.0	3.3703747050960633	0.0
12	7.699841691254828E-5	0.0	0.0	3.7780043242310937	0.0
13	7.699841691254828E-5	0.0	0.0	3.937160051989331	0.0
14	7.699841691254828E-5	0.0	0.0	3.989518975489864	0.0
15	7.699841691254828E-5	0.0	0.0	4.1023216562667475	0.0
16	7.699841691254828E-5	0.0	0.0	4.297743638390795	0.0
17	7.699841691254828E-5	0.0	0.0	4.52997086379904	0.0
18	7.699841691254828E-5	0.0	0.0	4.8254907879094	0.0
19	7.699841691254828E-5	0.0	0.0	5.0069760565722765	0.0
20	7.699841691254828E-5	0.0	0.0	5.201397059276461	0.0
21	1.5399683382509656E-4	0.0	0.0	5.475126431400571	0.0
22	1.5399683382509656E-4	0.0	0.0	5.753167714871783	0.0
23	1.5399683382509656E-4	0.0	0.0	6.023586155068652	0.0
24	1.5399683382509656E-4	0.0	0.0	6.225706999464091	0.0
25	1.5399683382509656E-4	0.0	0.0	6.43475770138166	0.0
26	1.5399683382509656E-4	0.0	0.0	6.599688310408338	0.0
27	1.5399683382509656E-4	0.0	0.0	6.786332473004355	0.0
28	1.5399683382509656E-4	0.0	0.0	6.980522480457802	0.0
29	1.5399683382509656E-4	0.0	0.0	7.218370590300664	0.0
30	1.5399683382509656E-4	0.0	0.0	7.455525714391312	0.0
31	1.5399683382509656E-4	0.0	0.0	7.684596004706143	0.0
32	1.5399683382509656E-4	0.0	0.0	7.892722725620761	0.0
33	1.5399683382509656E-4	0.0	0.0	8.094458577931638	0.0
34	1.5399683382509656E-4	0.0	0.0	8.331767698856112	0.0
35	1.5399683382509656E-4	0.0	0.0	8.5918683511867	0.0
36	1.5399683382509656E-4	0.0	0.0	8.801612038856481	0.0
37	1.5399683382509656E-4	0.0	0.0	9.026755409908771	0.0
38	1.5399683382509656E-4	0.0	0.0	9.290397989417338	0.0
39	1.5399683382509656E-4	0.0	0.0	9.712811304599578	0.0
40	1.5399683382509656E-4	0.0	0.0	9.969293031335276	0.0
41	1.5399683382509656E-4	0.0	0.0	10.22084685938857	0.0
42	1.5399683382509656E-4	0.0	0.0	10.472631682692604	0.0
43	1.5399683382509656E-4	0.0	0.0	10.746669048484364	0.0
44	1.5399683382509656E-4	0.0	0.0	10.9843631614934	0.0
45	1.5399683382509656E-4	0.0	0.0	11.20373165127725	0.0
46	1.5399683382509656E-4	0.0	0.0	11.442888734207624	0.0
47	1.5399683382509656E-4	0.0	0.0	11.700602435613924	0.0
48	1.5399683382509656E-4	0.0	0.0	11.951925268416481	0.0
49	1.5399683382509656E-4	0.0	0.0	12.215336852674309	0.0
50	1.5399683382509656E-4	0.0	0.0	12.469431628485719	0.0
51	1.5399683382509656E-4	0.0	0.0	12.726529342556717	0.0
52	1.5399683382509656E-4	0.0	0.0	13.050692677758546	0.0
53	2.3099525073764485E-4	0.0	0.0	13.339359742763689	0.0
54	2.3099525073764485E-4	0.0	0.0	13.630413758693122	0.0
55	2.3099525073764485E-4	0.0	0.0	13.908301045330507	0.0
56	2.3099525073764485E-4	0.0	0.0	14.191578221151772	0.0
57	2.3099525073764485E-4	0.0	0.0	14.471390468211974	0.0
58	2.3099525073764485E-4	0.0	0.0	14.743040883079443	0.0
59	2.3099525073764485E-4	0.0	0.0	15.007761440424785	0.0
60	2.3099525073764485E-4	0.0	0.0	15.308517256885198	0.0
61	2.3099525073764485E-4	0.0	0.0	15.597646312391817	0.0
62	2.3099525073764485E-4	0.0	0.0	15.935669362637904	0.0
63	2.3099525073764485E-4	0.0	0.0	16.293942996531992	0.0
64	2.3099525073764485E-4	0.0	0.0	16.616874357063217	0.0
65	2.3099525073764485E-4	0.0	0.0	16.91970913078027	0.0
66	2.3099525073764485E-4	0.0	0.0	17.183197713455012	0.0
67	2.3099525073764485E-4	0.0	0.0	17.46809185603144	0.0
68	2.3099525073764485E-4	0.0	0.0	17.82382454216741	0.0
69	3.079936676501931E-4	0.0	0.0	18.324699244183538	0.0
70	3.079936676501931E-4	0.0	0.0	18.65648542265971	0.0
71	3.079936676501931E-4	0.0	0.0	18.97533586709457	0.0
72	3.079936676501931E-4	0.0	0.0	19.283252536327854	0.0
73	3.079936676501931E-4	0.0	0.0	19.592093186564085	0.0
74	3.079936676501931E-4	0.0	0.0	19.93589111807861	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATATCG	15	0.0013098295	80.0	1
CTCGCGG	30	4.06053E-8	80.0	2
CGTTTTT	4305	0.0	71.823456	1
ATTCGCG	45	1.1823431E-10	71.111115	1
GCGTAAG	170	0.0	68.23529	1
TAGGGCG	260	0.0	67.69231	5
TAGGGTA	1260	0.0	67.61905	5
CGAGGGA	635	0.0	66.77165	4
AGGGATT	1420	0.0	66.76057	6
GTAGGGT	1255	0.0	65.65737	4
TCGATTG	165	0.0	65.454544	1
ATCGTAG	55	9.094947E-12	65.454544	1
TCGATAG	135	0.0	65.18518	1
ACGGGTA	160	0.0	65.0	5
AGTAGGG	2735	0.0	64.93602	3
CGTAGGG	760	0.0	64.73684	3
TTGTACG	155	0.0	64.51613	1
ATAGCCG	25	1.2355868E-4	64.0	1
CGCGCGG	50	3.0013325E-10	64.0	2
CTTTACG	25	1.2355868E-4	64.0	1
>>END_MODULE