FastQCFastQC Report
Thu 26 May 2016
SRR1547837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547837_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1573606
Sequences flagged as poor quality0
Sequence length86
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124300.7899054782455074No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC351.2914825E-980.09
CGTTTTT57150.074.470691
TTCGTAG1250.070.41
CGAACGG1200.070.02
CGGGATA5850.069.059836
TTCGCGA351.18176104E-768.571431
TAGGGAC21050.068.218525
TTGCGAG1600.067.51
ACGTCGG303.7962363E-666.6666641
TACGGGA9450.066.0317464
GGGCGAT10850.065.622127
ACGGGAC7200.065.555565
GGCACCG2750.065.4545448
GCGAACG800.065.01
CCGACGG503.0013325E-1064.000012
TGGTACG750.064.01
CCGATCC15300.063.79084880
ACGGGAT11400.063.5087745
GTAGGGA28600.063.4965024
TCGTAGG2650.063.396232
Produced by FastQC (version 0.11.5)