##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1547837_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1573606
Sequences flagged as poor quality	0
Sequence length	86
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.06787721958356	33.0	31.0	34.0	31.0	34.0
2	32.48894195878765	34.0	31.0	34.0	31.0	34.0
3	32.59278053083173	34.0	31.0	34.0	31.0	34.0
4	36.110916582676985	37.0	35.0	37.0	35.0	37.0
5	36.07906998321054	37.0	35.0	37.0	35.0	37.0
6	36.14184808649687	37.0	36.0	37.0	35.0	37.0
7	36.380517740781364	37.0	37.0	37.0	35.0	37.0
8	36.39669142085122	37.0	37.0	37.0	35.0	37.0
9	38.214795825638696	39.0	39.0	39.0	37.0	39.0
10-11	37.72369417757685	39.0	37.5	39.0	35.0	39.0
12-13	37.01766071049551	39.0	35.0	39.0	33.5	39.0
14-15	38.19833808462855	40.0	36.0	41.0	33.5	41.0
16-17	38.36479271177156	40.0	36.0	41.0	34.0	41.0
18-19	38.242416780312226	40.0	36.0	41.0	34.0	41.0
20-21	38.061531603209446	40.0	35.0	41.0	34.0	41.0
22-23	37.88408248316288	40.0	35.0	41.0	34.0	41.0
24-25	37.84460690922633	40.0	35.0	41.0	34.0	41.0
26-27	37.838450984553944	40.0	35.0	41.0	34.0	41.0
28-29	37.779834660010195	40.0	35.0	41.0	34.0	41.0
30-31	37.625763374059325	40.0	35.0	41.0	33.5	41.0
32-33	37.31778157937883	40.0	35.0	41.0	33.0	41.0
34-35	37.04786204424742	40.0	35.0	41.0	32.5	41.0
36-37	36.87260756504487	40.0	35.0	41.0	32.0	41.0
38-39	36.78745441997552	39.5	35.0	41.0	32.0	41.0
40-41	36.68105103818872	39.0	35.0	41.0	31.0	41.0
42-43	36.62821633877857	39.0	35.0	41.0	31.0	41.0
44-45	36.5110186412609	39.0	35.0	41.0	31.0	41.0
46-47	36.392818469172084	39.0	35.0	41.0	31.0	41.0
48-49	36.29918639100258	39.0	35.0	41.0	31.0	41.0
50-51	36.08773511285544	38.0	35.0	40.0	30.5	41.0
52-53	35.903498715688684	38.0	35.0	40.0	30.0	41.0
54-55	35.73591547058158	38.0	35.0	40.0	29.5	41.0
56-57	35.44659177710304	37.0	35.0	40.0	29.0	41.0
58-59	35.20301333370615	37.0	35.0	40.0	28.5	41.0
60-61	34.851269313919744	36.0	34.0	39.5	28.0	41.0
62-63	34.466229475484965	36.0	34.0	39.0	27.0	41.0
64-65	34.12279852771279	35.0	34.0	39.0	26.0	40.0
66-67	33.84089441702688	35.0	34.0	38.0	26.0	40.0
68-69	33.39604577003392	35.0	33.5	37.5	25.5	40.0
70-71	33.09877567828288	35.0	33.0	37.0	25.0	39.0
72-73	32.81710415440714	35.0	33.0	36.5	24.5	39.0
74-75	32.50913983551156	35.0	33.0	36.0	24.0	38.5
76-77	30.54669180214107	33.0	30.0	34.5	20.0	36.0
78-79	31.559269918899645	34.0	31.5	35.0	20.5	37.0
80-81	31.712069285450106	35.0	32.0	35.0	20.0	36.5
82-83	31.521241022212678	35.0	32.0	35.0	20.0	36.0
84-85	31.304438340982433	34.5	32.0	35.0	19.5	36.0
86	30.89933058211522	34.0	31.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	5.0
11	13.0
12	28.0
13	72.0
14	195.0
15	548.0
16	1402.0
17	3156.0
18	6955.0
19	11433.0
20	12871.0
21	12241.0
22	10688.0
23	9510.0
24	9649.0
25	10800.0
26	12597.0
27	15212.0
28	18814.0
29	23510.0
30	29807.0
31	37803.0
32	47974.0
33	63581.0
34	163829.0
35	152438.0
36	127406.0
37	241234.0
38	374594.0
39	174832.0
40	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.31978017369024	24.12586123845486	29.316232907093642	14.238125680761257
2	30.81476557664371	25.554808509881127	31.395724215591454	12.234701697883715
3	29.16657663989588	25.057670090225887	33.33826891864927	12.437484351228962
4	26.198235136368314	27.201027449056497	32.86121176457131	13.739525650003875
5	23.861436725584422	31.747845394590513	31.18207480144331	13.208643078381755
6	21.69329552632616	41.34255970045869	26.021761482861656	10.942383290353494
7	89.5504338443041	2.93809250854407	6.215278792785488	1.2961948543663406
8	90.89549734812907	2.2456701359806712	5.678104938593269	1.1807275772969854
9	87.03646274861687	4.824142765088593	6.620589906240825	1.5188045800537109
10-11	38.79541638758368	29.790652806356867	20.139030989968266	11.274899816091194
12-13	27.541265094312045	26.731119479717286	29.366658490117604	16.360956935853068
14-15	16.10307789878788	31.819400790286767	35.42707005438464	16.65045125654071
16-17	21.690054562577927	26.09245897638926	32.87674932607019	19.340737134962627
18-19	23.799921962676805	28.499192300995297	30.202700040543824	17.498185695784077
20-21	25.631098254582152	28.427541582835858	29.580562097500902	16.360798065081095
22-23	20.94564967342524	25.236367934540162	32.20723611882517	21.61074627320943
24-25	19.001230295258154	28.82208125795148	34.16983031330587	18.006858133484492
26-27	17.835976731151256	33.58486813090443	30.346192121789063	18.232963016155253
28-29	16.508611431323978	28.880069089721317	36.22485552291997	18.38646395603474
30-31	21.799389427849157	28.473868299942932	31.229100549947063	18.497641722260845
32-33	23.889588626377886	28.587016063741494	28.697399476107744	18.825995833772875
34-35	19.390590783207486	29.23479574938072	30.538044466022622	20.836569001389165
36-37	21.930076524873442	29.572936300446234	30.194692953636427	18.302294221043898
38-39	19.48445163528863	31.492031677560966	28.892175042545592	20.13134164460481
40-41	20.51749294296031	27.553116853901166	29.667941022085582	22.261449181052946
42-43	20.781250198588467	28.470627336194703	28.176652859737445	22.57146960547939
44-45	19.406509634559097	31.247688430267807	29.101916235703218	20.243885699469878
46-47	20.990292360349414	29.307780982024727	28.901230676548007	20.800695981077855
48-49	20.806955489493557	28.16629448540486	30.97122151288188	20.055528512219702
50-51	19.086099061645672	31.200630907609657	29.389440558818407	20.323829471926263
52-53	21.62212142048264	27.381059807855333	29.72116273069625	21.27565604096578
54-55	20.945395480190086	28.393956301640944	30.422259447409328	20.238388770759645
56-57	21.734252411340577	26.204272225703257	30.2598617442994	21.801613618656766
58-59	22.106041791909792	28.240487135915853	29.053333553634136	20.60013751854022
60-61	23.31533433400737	29.734094811534778	27.59092809763054	19.359642756827313
62-63	22.719219423413485	27.078410987248397	30.369196609570633	19.83317297976749
64-65	20.32973946464363	30.306823944494365	30.138452700358286	19.224983890503722
66-67	18.752375118040984	30.600480679407678	27.077807278314904	23.569336924236435
68-69	19.171762181893055	31.796269205887622	26.77588926325904	22.256079348960288
70-71	19.195592797688874	30.560953631341008	29.02025665890954	21.22319691206058
72-73	22.3113346034522	30.369959189276095	27.50335852811949	19.815347679152215
74-75	21.0896183669864	31.799065331474335	25.704369454615705	21.406946846923564
76-77	23.876783642156933	29.529373934771474	27.024299602314684	19.56954282075691
78-79	18.929706673716293	30.022667681744984	29.384928628894396	21.662697015644323
80-81	17.705607375670912	29.531185061571957	29.756559138691642	23.00664842406549
82-83	19.346106967055285	28.43278431831094	30.90560788405738	21.315500830576394
84-85	17.44305753790974	28.25297437859286	29.47338787472849	24.830580208768904
86	18.931295381436016	28.261013239654652	30.346859379031343	22.460831999877986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	777.0
1	891.0
2	4342.0
3	4687.0
4	1822.5
5	2314.0
6	3031.0
7	3384.0
8	3698.5
9	4131.5
10	4068.5
11	3840.5
12	3767.0
13	3684.0
14	3628.0
15	3692.0
16	3728.5
17	4184.5
18	4357.5
19	4237.5
20	4738.0
21	5048.0
22	5313.5
23	5673.5
24	7012.0
25	8847.0
26	10306.0
27	12637.0
28	17290.0
29	20480.0
30	22264.0
31	26525.0
32	31855.5
33	35265.5
34	38718.5
35	43475.5
36	45336.0
37	45778.0
38	49546.0
39	54340.0
40	60747.5
41	70549.0
42	77039.0
43	78667.0
44	83074.5
45	89113.0
46	91556.0
47	90672.0
48	89504.5
49	82724.0
50	75415.0
51	65523.5
52	51949.5
53	45384.0
54	41084.0
55	37331.0
56	32241.0
57	29487.0
58	25860.0
59	19537.5
60	13549.0
61	8872.5
62	6175.0
63	3932.0
64	3003.0
65	2394.5
66	1437.5
67	1016.5
68	682.0
69	364.5
70	293.5
71	278.0
72	189.0
73	103.0
74	82.5
75	48.0
76	31.0
77	27.0
78	21.5
79	14.0
80	12.5
81	11.0
82	8.0
83	3.5
84	2.5
85	2.5
86	2.0
87	1.0
88	1.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	0.0
84-85	0.0
86	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
86	1573606.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.11852951810853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.53473717167894	15.014686908136394
2	7.97843849698354	3.0507202382598657
3	2.656208141495554	1.5234838127826884
4	1.3191158318992025	1.0087821987987677
5	0.8523629015424886	0.8147962646640351
6	0.598027824107134	0.6860047564705475
7	0.4542936746580327	0.6079798920187715
8	0.3664276051849339	0.5604445588782381
9	0.3106183409036989	0.5344709327489938
>10	4.069233114393078	18.785755319447627
>50	1.6252898641890154	22.546517001169207
>100	1.2302120586453222	33.327396544193135
>500	0.003020984591488985	0.36649887578322965
>1k	0.001678324773049436	0.37477734802557544
>5k	0.0	0.0
>10k+	3.3566495460988724E-4	0.7976853486229285
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12430	0.7899054782455074	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03196479932079568	0.0
2	6.354830878885821E-5	0.0	0.0	0.09589439796238702	0.0
3	6.354830878885821E-5	0.0	0.0	0.14476304742101898	0.0
4	6.354830878885821E-5	0.0	0.0	0.23385777634299817	0.0
5	6.354830878885821E-5	0.0	0.0	0.45659459864794616	0.0
6	6.354830878885821E-5	0.0	0.0	0.6342121217128048	0.0
7	6.354830878885821E-5	0.0	0.0	0.7713493720791609	0.0
8	6.354830878885821E-5	0.0	0.0	1.0758093194865805	0.0
9	6.354830878885821E-5	0.0	0.0	1.1736101667126333	0.0
10	6.354830878885821E-5	0.0	0.0	1.3670512186659176	0.0
11	6.354830878885821E-5	0.0	0.0	1.7116736972278956	0.0
12	6.354830878885821E-5	0.0	0.0	1.9914133525164495	0.0
13	6.354830878885821E-5	0.0	0.0	2.0826687239372497	0.0
14	6.354830878885821E-5	0.0	0.0	2.1171754556096	0.0
15	6.354830878885821E-5	0.0	0.0	2.168141199258264	0.0
16	6.354830878885821E-5	0.0	0.0	2.2834178314012528	0.0
17	6.354830878885821E-5	0.0	0.0	2.442987634770076	0.0
18	6.354830878885821E-5	0.0	0.0	2.6234012834216442	0.0
19	6.354830878885821E-5	0.0	0.0	2.7565349903343024	0.0
20	6.354830878885821E-5	0.0	0.0	2.8940535305533914	0.0
21	6.354830878885821E-5	0.0	0.0	3.062901387005388	0.0
22	6.354830878885821E-5	0.0	0.0	3.2686072625549216	0.0
23	6.354830878885821E-5	0.0	0.0	3.4704366912683353	0.0
24	6.354830878885821E-5	0.0	0.0	3.61043361553019	0.0
25	6.354830878885821E-5	0.0	0.0	3.737657329725484	0.0
26	6.354830878885821E-5	0.0	0.0	3.860750403849502	0.0
27	6.354830878885821E-5	0.0	0.0	3.996362494804926	0.0
28	6.354830878885821E-5	0.0	0.0	4.138964899727124	0.0
29	6.354830878885821E-5	0.0	0.0	4.309210818972475	0.0
30	6.354830878885821E-5	0.0	0.0	4.511612182464988	0.0
31	6.354830878885821E-5	0.0	0.0	4.6800787490642515	0.0
32	6.354830878885821E-5	0.0	0.0	4.832975980010244	0.0
33	6.354830878885821E-5	0.0	0.0	4.9904486891890345	0.0
34	6.354830878885821E-5	0.0	0.0	5.172133304016381	0.0
35	6.354830878885821E-5	0.0	0.0	5.372691766554016	0.0
36	6.354830878885821E-5	0.0	0.0	5.543128330725734	0.0
37	6.354830878885821E-5	0.0	0.0	5.723986817538825	0.0
38	6.354830878885821E-5	0.0	0.0	5.934903654409045	0.0
39	6.354830878885821E-5	0.0	0.0	6.257093579968556	0.0
40	6.354830878885821E-5	0.0	0.0	6.460702361328058	0.0
41	6.354830878885821E-5	0.0	0.0	6.661578565409639	0.0
42	1.2709661757771641E-4	0.0	0.0	6.877897008526912	0.0
43	1.2709661757771641E-4	0.0	0.0	7.072545478347185	0.0
44	1.2709661757771641E-4	0.0	0.0	7.268020076181712	0.0
45	1.906449263665746E-4	0.0	0.0	7.463558222325029	0.0
46	1.906449263665746E-4	0.0	0.0	7.6724415133140065	0.0
47	1.906449263665746E-4	0.0	0.0	7.9045199370109165	0.0
48	1.906449263665746E-4	0.0	0.0	8.11969451056999	0.0
49	1.906449263665746E-4	0.0	0.0	8.337792306333352	0.0
50	1.906449263665746E-4	0.0	0.0	8.566502669664452	0.0
51	1.906449263665746E-4	0.0	0.0	8.7903833615276	0.0
52	2.5419323515543283E-4	0.0	0.0	9.068852050640377	0.0
53	2.5419323515543283E-4	0.0	0.0	9.30182015066033	0.0
54	2.5419323515543283E-4	0.0	0.0	9.540316953544915	0.0
55	2.5419323515543283E-4	0.0	0.0	9.785422780543541	0.0
56	2.5419323515543283E-4	0.0	0.0	10.025762484383003	0.0
57	2.5419323515543283E-4	0.0	0.0	10.264322835576376	0.0
58	2.5419323515543283E-4	0.0	0.0	10.500150609491829	0.0
59	2.5419323515543283E-4	0.0	0.0	10.749387076561732	0.0
60	3.812898527331492E-4	0.0	0.0	11.01660771501888	0.0
61	3.812898527331492E-4	0.0	0.0	11.278045457376242	0.0
62	3.812898527331492E-4	0.0	0.0	11.572782513538968	0.0
63	3.812898527331492E-4	0.0	0.0	11.878958265283686	0.0
64	4.4483816152200744E-4	0.0	0.0	12.160667918144695	0.0
65	4.4483816152200744E-4	0.0	0.0	12.430621133879765	0.0
66	4.4483816152200744E-4	0.0	0.0	12.691169199914082	0.0
67	4.4483816152200744E-4	0.0	0.0	12.959088869767909	0.0
68	4.4483816152200744E-4	0.0	0.0	13.267298167393871	0.0
69	4.4483816152200744E-4	0.0	0.0	13.647317053951243	0.0
70	4.4483816152200744E-4	0.0	0.0	13.934047023206572	0.0
71	4.4483816152200744E-4	0.0	0.0	14.20463572202953	0.0
72	5.083864703108657E-4	0.0	0.0	14.488378920771781	0.0
73	5.083864703108657E-4	0.0	0.0	14.759984392535362	0.0
74	5.083864703108657E-4	0.0	0.0	15.064444339942781	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	35	1.2914825E-9	80.0	9
CGTTTTT	5715	0.0	74.47069	1
TTCGTAG	125	0.0	70.4	1
CGAACGG	120	0.0	70.0	2
CGGGATA	585	0.0	69.05983	6
TTCGCGA	35	1.18176104E-7	68.57143	1
TAGGGAC	2105	0.0	68.21852	5
TTGCGAG	160	0.0	67.5	1
ACGTCGG	30	3.7962363E-6	66.666664	1
TACGGGA	945	0.0	66.031746	4
GGGCGAT	1085	0.0	65.62212	7
ACGGGAC	720	0.0	65.55556	5
GGCACCG	275	0.0	65.454544	8
GCGAACG	80	0.0	65.0	1
CCGACGG	50	3.0013325E-10	64.00001	2
TGGTACG	75	0.0	64.0	1
CCGATCC	1530	0.0	63.790848	80
ACGGGAT	1140	0.0	63.508774	5
GTAGGGA	2860	0.0	63.496502	4
TCGTAGG	265	0.0	63.39623	2
>>END_MODULE