FastQCFastQC Report
Thu 26 May 2016
SRR1547836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1547836_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2595271
Sequences flagged as poor quality0
Sequence length86
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102770.39598947470225654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80000.070.81
CGAGGGA19200.066.0416644
TACGGGA13100.065.648854
TAACGCG459.429641E-962.2222251
CGTAAGG4500.062.222222
AGGGATT53450.061.7399446
ACGGGAT13350.061.722855
GCGGGAT21100.061.232235
CCGATCC17750.061.07042380
GCGGGTC4500.060.4444435
CGGGATA7950.060.3773576
TAGGGAC36500.060.0547945
CGAACGG602.1827873E-1159.9999962
GCGATAC1200.059.9999969
GGGCGAT17500.059.8857157
TAGGGTA22850.059.51865
TAGGGAT55350.059.3315285
TAAGGGA40450.059.233624
AAGGGAT53350.059.2314875
ATAGGGA41950.058.927294
Produced by FastQC (version 0.11.5)